Immunoassay based on hot electron induced electrochemiluminescense on disposable cell chips with printed electrodes

Nur-E-Habiba Nur-E-Habiba; Kalle Salminen; Päivi Grönroos; Esko Kauppinen; Veikko Sariola; Sakari Kulmala

Abstract

This paper reports utilization of Hot Electron-induced Electrochemiluminescence (HECL) on inexpensive disposable printed electrode chips for the first time. HECL method typically utilizes luminophores as labels in sensitive bioaffinity-based assays. Here we demonstrate HECL, as a novel approach by using a widely used fluorescent probe - fluorescein isothiocyanate (FITC) as luminophore label for human C-reactive protein (hCRP)assay. Our work results in a combined benefit of the integration of HECL on disposable electrode chip and the utilization of the low-cost FITC, resulting in successful real-world heterogeneous immunoassay development, and thus paving the way for being used in point of care devices.

Original language	English
Pages	1268-1269
Number of pages	2
Publication status	Published - 2019
Publication type	Not Eligible
Event	International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS - Basel, Switzerland Duration: 27 Oct 2019 → 31 Oct 2019 Conference number: 23rd

Conference

Conference	International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS
Country/Territory	Switzerland
City	Basel
Period	27/10/19 → 31/10/19

Keywords

Hot Electron-induced Electrochemiluminescence
Human C-reactive Protein
Immunoassay

ASJC Scopus subject areas

Bioengineering
Chemical Engineering (miscellaneous)

Cite this

@conference{4956e69b5758406ab94990c1f052b326,

title = "Immunoassay based on hot electron induced electrochemiluminescense on disposable cell chips with printed electrodes",

abstract = "This paper reports utilization of Hot Electron-induced Electrochemiluminescence (HECL) on inexpensive disposable printed electrode chips for the first time. HECL method typically utilizes luminophores as labels in sensitive bioaffinity-based assays. Here we demonstrate HECL, as a novel approach by using a widely used fluorescent probe - fluorescein isothiocyanate (FITC) as luminophore label for human C-reactive protein (hCRP)assay. Our work results in a combined benefit of the integration of HECL on disposable electrode chip and the utilization of the low-cost FITC, resulting in successful real-world heterogeneous immunoassay development, and thus paving the way for being used in point of care devices.",

keywords = "Hot Electron-induced Electrochemiluminescence, Human C-reactive Protein, Immunoassay",

author = "Nur-E-Habiba and Kalle Salminen and P{\"a}ivi Gr{\"o}nroos and Esko Kauppinen and Veikko Sariola and Sakari Kulmala",

note = "Funding Information: We would like to thank postdoctoral researcher Dr. Rokon Uddin from Micronova (Aalto University) for helping us to use their cleanroom resources. The work was financially supported by Jenny and Antti Wihuri Foundation and Biometro Co. Ltd, South Korea. Publisher Copyright: {\textcopyright} 2019 CBMS-0001. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.; International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS ; Conference date: 27-10-2019 Through 31-10-2019",

year = "2019",

language = "English",

pages = "1268--1269",

}

Immunoassay based on hot electron induced electrochemiluminescense on disposable cell chips with printed electrodes. / Nur-E-Habiba; Salminen, Kalle; Grönroos, Päivi et al.
2019. 1268-1269 Abstract from International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS, Basel, Switzerland.

Research output: Other conference contribution › Abstract › Scientific

TY - CONF

T1 - Immunoassay based on hot electron induced electrochemiluminescense on disposable cell chips with printed electrodes

AU - Nur-E-Habiba, null

AU - Salminen, Kalle

AU - Grönroos, Päivi

AU - Kauppinen, Esko

AU - Sariola, Veikko

AU - Kulmala, Sakari

N1 - Conference code: 23rd

PY - 2019

Y1 - 2019

N2 - This paper reports utilization of Hot Electron-induced Electrochemiluminescence (HECL) on inexpensive disposable printed electrode chips for the first time. HECL method typically utilizes luminophores as labels in sensitive bioaffinity-based assays. Here we demonstrate HECL, as a novel approach by using a widely used fluorescent probe - fluorescein isothiocyanate (FITC) as luminophore label for human C-reactive protein (hCRP)assay. Our work results in a combined benefit of the integration of HECL on disposable electrode chip and the utilization of the low-cost FITC, resulting in successful real-world heterogeneous immunoassay development, and thus paving the way for being used in point of care devices.

AB - This paper reports utilization of Hot Electron-induced Electrochemiluminescence (HECL) on inexpensive disposable printed electrode chips for the first time. HECL method typically utilizes luminophores as labels in sensitive bioaffinity-based assays. Here we demonstrate HECL, as a novel approach by using a widely used fluorescent probe - fluorescein isothiocyanate (FITC) as luminophore label for human C-reactive protein (hCRP)assay. Our work results in a combined benefit of the integration of HECL on disposable electrode chip and the utilization of the low-cost FITC, resulting in successful real-world heterogeneous immunoassay development, and thus paving the way for being used in point of care devices.

KW - Hot Electron-induced Electrochemiluminescence

KW - Human C-reactive Protein

KW - Immunoassay

UR - http://www.scopus.com/inward/record.url?scp=85094976169&partnerID=8YFLogxK

M3 - Abstract

AN - SCOPUS:85094976169

SP - 1268

EP - 1269

T2 - International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS

Y2 - 27 October 2019 through 31 October 2019

ER -