Immunoassay for intact amino-terminal propeptide of human type I procollagen

Jukka Melkko, Saila Kauppila, Seija Niemi, Leila Risteli, Kari Haukipuro, Arja Jukkola, Juha Risteli

Research output: Contribution to journalArticleScientificpeer-review

Abstract

We have developed quantitative immunoassays for the intact, trimeric ammo-terminal propeptide of human type I procollagen (PINP) and its Coll domain. Intact PINP was isolated from the pleural fluids of cancer patients by a combination of ion-exchange, gel-filtration, and reversed-phasc chromatographies. The ammo-terminal Coll domain of PINP was isolated after bacterial collagcnasc treatment of the heat-denatured trimeric propeptide. For the intact PINP assay we used a polyclonal antibody with only 1.2% cross-reaction with the monomeric Coll domain. In human serum, this assay detects only one peak of PINP antigenicity that has the size of known intact PINP. Under similar conditions, an assay for the Coll domain of PINP recognized two circulating antigens. The biological relevance was further verified in wound fluid. Intcrassay and intraassay CVs were 3.1-9.3% for values within the reference intervals (mean ± 2SD) for intact PINP in serum, which were 19-84 μg/L for women and 20-76 μg/L- for men.
Original languageEnglish
Pages (from-to)947-954
Number of pages8
JournalClinical Chemistry
Volume42
Issue number6
DOIs
Publication statusPublished - 1996
Externally publishedYes
Publication typeA1 Journal article-refereed

Keywords

  • Bone
  • Cancer
  • Coll domain
  • Collagen
  • Pleural fluid

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