Impact of borosilicate bioactive glass scaffold processing and reactivity on in-vitro dissolution properties

Agata Szczodra; Jenna M. Tainio; Amel Houaoui; Hongfei Liu; Juuso Pohjola; Susanna Miettinen; Delia S. Brauer; Jonathan Massera

doi:10.1016/j.mtcomm.2023.105984

Impact of borosilicate bioactive glass scaffold processing and reactivity on in-vitro dissolution properties

Agata Szczodra, Jenna M. Tainio, Amel Houaoui, Hongfei Liu, Juuso Pohjola, Susanna Miettinen, Delia S. Brauer, Jonathan Massera

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Abstract

In this study, bulk borosilicate glasses and 3D scaffolds (processed by the burn-off technique and by robocasting) were synthesized to investigate the impact of the manufacturing method, glass composition and preincubation time on in vitro dissolution and cell response. The studied compositions are based on commercial bioactive glass S53P4 (BonAlive) where 12.5% SiO₂ has been replaced by B₂O (labelled B12.5), and part of the CaO is replaced with MgO and SrO (labelled B12.5-Mg-Sr). First, the impact of the processing and glass composition, on the dissolution rate, was assessed. As expected, scaffolds were found to exhibit faster dissolution, due to the increased surface area, when compared to the bulk glass. Furthermore, the 3D printed scaffolds were found to dissolve faster than the burn-off scaffolds. Moreover, scaffolds made from B12.5-Mg-Sr glass composition exhibited slower ion release and precipitation of calcium phosphate (CaP) layer, when compared to B12.5, due to the stabilizing effect of Mg and Sr. Finally, dynamic condition produces lower ion releases that static condition and could be more optimal for in vitro cell growth. Secondly, in culture with murine MC3T3-E1 cells, it was shown that 3 days preincubation would be optimal to decrease the burst of ions that is known to lead to cell death. However, it was found that MC3T3-E1 survived and proliferated only in presence of B12.5-Mg-Sr scaffolds. Finally, it was shown that despite scaffolds having different porosities, they had no significant difference on human adipose-derived stem cells (hADSCs) survival. This manuscript brings new information on 1) the impact of material design (porosity) and composition on dissolution kinetic sand reactivity, 2) the impact of static vs dynamic testing on in-vitro dissolution and 3) the impact of materials’ pre-incubation on cell behavior.

Original language	English
Article number	105984
Number of pages	20
Journal	Materials Today Communications
Volume	35
DOIs	https://doi.org/10.1016/j.mtcomm.2023.105984
Publication status	Published - Jun 2023
Publication type	A1 Journal article-refereed

Funding

Authors would like to thank the Academy of Finland (research grant #331924 ) for financial support of the AS as well as the DAAD-Finland mobility program (grant #295962 ) for the mobility of JT and JP to Jena university.

Keywords

3D printing
Borosilicate bioactive glasses
Human cells
In vitro dissolution
Scaffolds

Publication forum classification

Publication forum level 1

ASJC Scopus subject areas

General Materials Science
Mechanics of Materials
Materials Chemistry

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10.1016/j.mtcomm.2023.105984Licence: CC BY

1-s2.0-S235249282300675X-mainFinal published version, 16 MBLicence: CC BY

https://urn.fi/URN:NBN:fi:tuni-202305105545Licence: CC BY

Cite this

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title = "Impact of borosilicate bioactive glass scaffold processing and reactivity on in-vitro dissolution properties",

abstract = "In this study, bulk borosilicate glasses and 3D scaffolds (processed by the burn-off technique and by robocasting) were synthesized to investigate the impact of the manufacturing method, glass composition and preincubation time on in vitro dissolution and cell response. The studied compositions are based on commercial bioactive glass S53P4 (BonAlive) where 12.5% SiO2 has been replaced by B2O (labelled B12.5), and part of the CaO is replaced with MgO and SrO (labelled B12.5-Mg-Sr). First, the impact of the processing and glass composition, on the dissolution rate, was assessed. As expected, scaffolds were found to exhibit faster dissolution, due to the increased surface area, when compared to the bulk glass. Furthermore, the 3D printed scaffolds were found to dissolve faster than the burn-off scaffolds. Moreover, scaffolds made from B12.5-Mg-Sr glass composition exhibited slower ion release and precipitation of calcium phosphate (CaP) layer, when compared to B12.5, due to the stabilizing effect of Mg and Sr. Finally, dynamic condition produces lower ion releases that static condition and could be more optimal for in vitro cell growth. Secondly, in culture with murine MC3T3-E1 cells, it was shown that 3 days preincubation would be optimal to decrease the burst of ions that is known to lead to cell death. However, it was found that MC3T3-E1 survived and proliferated only in presence of B12.5-Mg-Sr scaffolds. Finally, it was shown that despite scaffolds having different porosities, they had no significant difference on human adipose-derived stem cells (hADSCs) survival. This manuscript brings new information on 1) the impact of material design (porosity) and composition on dissolution kinetic sand reactivity, 2) the impact of static vs dynamic testing on in-vitro dissolution and 3) the impact of materials{\textquoteright} pre-incubation on cell behavior.",

keywords = "3D printing, Borosilicate bioactive glasses, Human cells, In vitro dissolution, Scaffolds",

author = "Agata Szczodra and Tainio, {Jenna M.} and Amel Houaoui and Hongfei Liu and Juuso Pohjola and Susanna Miettinen and Brauer, {Delia S.} and Jonathan Massera",

note = "Funding Information: Authors would like to thank the Academy of Finland (research grant #331924 ) for financial support of the AS as well as the DAAD-Finland mobility program (grant #295962 ) for the mobility of JT and JP to Jena university. Publisher Copyright: {\textcopyright} 2023 The Authors",

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language = "English",

volume = "35",

journal = "Materials Today Communications",

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AU - Szczodra, Agata

AU - Tainio, Jenna M.

AU - Houaoui, Amel

AU - Liu, Hongfei

AU - Pohjola, Juuso

AU - Miettinen, Susanna

AU - Brauer, Delia S.

AU - Massera, Jonathan

N1 - Funding Information: Authors would like to thank the Academy of Finland (research grant #331924 ) for financial support of the AS as well as the DAAD-Finland mobility program (grant #295962 ) for the mobility of JT and JP to Jena university. Publisher Copyright: © 2023 The Authors

PY - 2023/6

Y1 - 2023/6

N2 - In this study, bulk borosilicate glasses and 3D scaffolds (processed by the burn-off technique and by robocasting) were synthesized to investigate the impact of the manufacturing method, glass composition and preincubation time on in vitro dissolution and cell response. The studied compositions are based on commercial bioactive glass S53P4 (BonAlive) where 12.5% SiO2 has been replaced by B2O (labelled B12.5), and part of the CaO is replaced with MgO and SrO (labelled B12.5-Mg-Sr). First, the impact of the processing and glass composition, on the dissolution rate, was assessed. As expected, scaffolds were found to exhibit faster dissolution, due to the increased surface area, when compared to the bulk glass. Furthermore, the 3D printed scaffolds were found to dissolve faster than the burn-off scaffolds. Moreover, scaffolds made from B12.5-Mg-Sr glass composition exhibited slower ion release and precipitation of calcium phosphate (CaP) layer, when compared to B12.5, due to the stabilizing effect of Mg and Sr. Finally, dynamic condition produces lower ion releases that static condition and could be more optimal for in vitro cell growth. Secondly, in culture with murine MC3T3-E1 cells, it was shown that 3 days preincubation would be optimal to decrease the burst of ions that is known to lead to cell death. However, it was found that MC3T3-E1 survived and proliferated only in presence of B12.5-Mg-Sr scaffolds. Finally, it was shown that despite scaffolds having different porosities, they had no significant difference on human adipose-derived stem cells (hADSCs) survival. This manuscript brings new information on 1) the impact of material design (porosity) and composition on dissolution kinetic sand reactivity, 2) the impact of static vs dynamic testing on in-vitro dissolution and 3) the impact of materials’ pre-incubation on cell behavior.

AB - In this study, bulk borosilicate glasses and 3D scaffolds (processed by the burn-off technique and by robocasting) were synthesized to investigate the impact of the manufacturing method, glass composition and preincubation time on in vitro dissolution and cell response. The studied compositions are based on commercial bioactive glass S53P4 (BonAlive) where 12.5% SiO2 has been replaced by B2O (labelled B12.5), and part of the CaO is replaced with MgO and SrO (labelled B12.5-Mg-Sr). First, the impact of the processing and glass composition, on the dissolution rate, was assessed. As expected, scaffolds were found to exhibit faster dissolution, due to the increased surface area, when compared to the bulk glass. Furthermore, the 3D printed scaffolds were found to dissolve faster than the burn-off scaffolds. Moreover, scaffolds made from B12.5-Mg-Sr glass composition exhibited slower ion release and precipitation of calcium phosphate (CaP) layer, when compared to B12.5, due to the stabilizing effect of Mg and Sr. Finally, dynamic condition produces lower ion releases that static condition and could be more optimal for in vitro cell growth. Secondly, in culture with murine MC3T3-E1 cells, it was shown that 3 days preincubation would be optimal to decrease the burst of ions that is known to lead to cell death. However, it was found that MC3T3-E1 survived and proliferated only in presence of B12.5-Mg-Sr scaffolds. Finally, it was shown that despite scaffolds having different porosities, they had no significant difference on human adipose-derived stem cells (hADSCs) survival. This manuscript brings new information on 1) the impact of material design (porosity) and composition on dissolution kinetic sand reactivity, 2) the impact of static vs dynamic testing on in-vitro dissolution and 3) the impact of materials’ pre-incubation on cell behavior.

KW - 3D printing

KW - Borosilicate bioactive glasses

KW - Human cells

KW - In vitro dissolution

KW - Scaffolds

U2 - 10.1016/j.mtcomm.2023.105984

DO - 10.1016/j.mtcomm.2023.105984

M3 - Article

AN - SCOPUS:85152964634

SN - 2352-4928

VL - 35

JO - Materials Today Communications

JF - Materials Today Communications

M1 - 105984

ER -

Impact of borosilicate bioactive glass scaffold processing and reactivity on in-vitro dissolution properties

Abstract

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