Improved biomass and lipid production in a mixotrophic culture of Chlorella sp. KR-1 with addition of coal-fired flue-gas

Ramasamy Praveenkumar; Bohwa Kim; Eunji Choi; Kyubock Lee; Ji Yeon Park; Jin Suk Lee; Young Chul Lee; You Kwan Oh

doi:10.1016/j.biortech.2014.08.112

Improved biomass and lipid production in a mixotrophic culture of Chlorella sp. KR-1 with addition of coal-fired flue-gas

Ramasamy Praveenkumar, Bohwa Kim, Eunji Choi, Kyubock Lee, Ji Yeon Park, Jin Suk Lee, Young Chul Lee, You Kwan Oh

Research output: Contribution to journal › Article › Scientific › peer-review

83 Citations (Scopus)

Abstract

Industrial CO₂-rich flue-gases, owing to their eco-toxicity, have yet to be practically exploited for microalgal biomass and lipid production. In this study, various autotrophic and mixotrophic culture modes for an oleaginous microalga, Chlorella sp. KR-1 were compared for the use in actual coal-fired flue-gas. Among the mixotrophic conditions tested, the fed-batch feedings of glucose and the supply of air in dark cycles showed the highest biomass (561mg/Ld) and fatty-acid methyl-ester (168mg/Ld) productivities. This growth condition also resulted in the maximal population of microalgae and the minimal population and types of KR-1-associated-bacterial species as confirmed by particle-volume-distribution and denaturing-gradient-gel-electrophoresis (DGGE) analyses. Furthermore, microalgal lipid produced was assessed, based on its fatty acid profile, to meet key biodiesel standards such as saponification, iodine, and cetane numbers.

Original language	English
Pages (from-to)	500-505
Number of pages	6
Journal	Bioresource Technology
Volume	171
DOIs	https://doi.org/10.1016/j.biortech.2014.08.112
Publication status	Published - 1 Nov 2014
Publication type	A1 Journal article-refereed

Keywords

Chlorella sp.
Coal-fired flue-gas
Fed-batch
Lipid
Mixotrophic culture

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Waste Management and Disposal
General Medicine

Access to Document

10.1016/j.biortech.2014.08.112

Cite this

@article{1593dd5698334043892fc68bdfd4a2dc,

title = "Improved biomass and lipid production in a mixotrophic culture of Chlorella sp. KR-1 with addition of coal-fired flue-gas",

abstract = "Industrial CO2-rich flue-gases, owing to their eco-toxicity, have yet to be practically exploited for microalgal biomass and lipid production. In this study, various autotrophic and mixotrophic culture modes for an oleaginous microalga, Chlorella sp. KR-1 were compared for the use in actual coal-fired flue-gas. Among the mixotrophic conditions tested, the fed-batch feedings of glucose and the supply of air in dark cycles showed the highest biomass (561mg/Ld) and fatty-acid methyl-ester (168mg/Ld) productivities. This growth condition also resulted in the maximal population of microalgae and the minimal population and types of KR-1-associated-bacterial species as confirmed by particle-volume-distribution and denaturing-gradient-gel-electrophoresis (DGGE) analyses. Furthermore, microalgal lipid produced was assessed, based on its fatty acid profile, to meet key biodiesel standards such as saponification, iodine, and cetane numbers.",

keywords = "Chlorella sp., Coal-fired flue-gas, Fed-batch, Lipid, Mixotrophic culture",

author = "Ramasamy Praveenkumar and Bohwa Kim and Eunji Choi and Kyubock Lee and Park, {Ji Yeon} and Lee, {Jin Suk} and Lee, {Young Chul} and Oh, {You Kwan}",

year = "2014",

month = nov,

day = "1",

doi = "10.1016/j.biortech.2014.08.112",

language = "English",

volume = "171",

pages = "500--505",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "ELSEVIER SCI LTD",

}

TY - JOUR

T1 - Improved biomass and lipid production in a mixotrophic culture of Chlorella sp. KR-1 with addition of coal-fired flue-gas

AU - Praveenkumar, Ramasamy

AU - Kim, Bohwa

AU - Choi, Eunji

AU - Lee, Kyubock

AU - Park, Ji Yeon

AU - Lee, Jin Suk

AU - Lee, Young Chul

AU - Oh, You Kwan

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Industrial CO2-rich flue-gases, owing to their eco-toxicity, have yet to be practically exploited for microalgal biomass and lipid production. In this study, various autotrophic and mixotrophic culture modes for an oleaginous microalga, Chlorella sp. KR-1 were compared for the use in actual coal-fired flue-gas. Among the mixotrophic conditions tested, the fed-batch feedings of glucose and the supply of air in dark cycles showed the highest biomass (561mg/Ld) and fatty-acid methyl-ester (168mg/Ld) productivities. This growth condition also resulted in the maximal population of microalgae and the minimal population and types of KR-1-associated-bacterial species as confirmed by particle-volume-distribution and denaturing-gradient-gel-electrophoresis (DGGE) analyses. Furthermore, microalgal lipid produced was assessed, based on its fatty acid profile, to meet key biodiesel standards such as saponification, iodine, and cetane numbers.

AB - Industrial CO2-rich flue-gases, owing to their eco-toxicity, have yet to be practically exploited for microalgal biomass and lipid production. In this study, various autotrophic and mixotrophic culture modes for an oleaginous microalga, Chlorella sp. KR-1 were compared for the use in actual coal-fired flue-gas. Among the mixotrophic conditions tested, the fed-batch feedings of glucose and the supply of air in dark cycles showed the highest biomass (561mg/Ld) and fatty-acid methyl-ester (168mg/Ld) productivities. This growth condition also resulted in the maximal population of microalgae and the minimal population and types of KR-1-associated-bacterial species as confirmed by particle-volume-distribution and denaturing-gradient-gel-electrophoresis (DGGE) analyses. Furthermore, microalgal lipid produced was assessed, based on its fatty acid profile, to meet key biodiesel standards such as saponification, iodine, and cetane numbers.

KW - Chlorella sp.

KW - Coal-fired flue-gas

KW - Fed-batch

KW - Lipid

KW - Mixotrophic culture

UR - http://www.scopus.com/inward/record.url?scp=84908669116&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2014.08.112

DO - 10.1016/j.biortech.2014.08.112

M3 - Article

C2 - 25227588

AN - SCOPUS:84908669116

SN - 0960-8524

VL - 171

SP - 500

EP - 505

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Improved biomass and lipid production in a mixotrophic culture of Chlorella sp. KR-1 with addition of coal-fired flue-gas

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this