Interleukin-6 and microRNA profiles induced by oral bacteria in human atheroma derived and healthy smooth muscle cells

Tanja Pessi, Leena E. Viiri, Emma Raitoharju, Nagora Astola, Ilkka Seppälä, Melanie Waldenberger, Kari Lounatmaa, Alun H. Davies, Terho Lehtimäki, Pekka J. Karhunen, Claudia Monaco

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

<p>Background: Atherosclerosis is an inflammatory disease with possible contributions from bacterial antigens. We aimed to investigate the role of oral bacteria as inducers of inflammatory cascades in smooth muscle cells from carotid endarterectomy patients (AthSMCs) and healthy controls (HSMCs). Findings: Inactivated Streptococcus mitis, S. sanguinis, S. gorgonii, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis were used to stimulate inflammation in HSMCs and AthSMCs. Tumor necrosis factor-α (TNFα) was used as a positive control in all stimulations. Interleukin-6 (IL-6) levels were determined from cell culture supernatants and microRNA expression profiles from cells after 24 h of bacterial stimulation. Genome wide expression (GWE) analyses were performed after 5 h stimulation. All studied bacteria induced pro inflammatory IL-6 production in both SMCs. The most powerful inducer of IL-6 was A. actinomycetemcomitans (p <0.001). Of the 84 studied miRNAs, expression of 9 miRNAs differed significantly (p ≤ 0.001) between HSMCs and AthSMCs stimulated with inactivated bacteria or TNFα. The data was divided into two groups: high IL-6 producers (A. actinomytectemcomititans and TNFα) and low IL-6 producers (streptococcal strains and P. gingivalis). The expression of 4 miRNAs (miR-181-5p, −186-5p, −28-5p and −155-5p) differed statistically significantly (p <0.001) between healthy HSMCs and AthSMCs in the low IL-6 producer group. According to multidimensional scaling, two gene expression clusters were seen: one in HSMCs and one AthSMCs. Conclusions: Our results suggest that inactivated oral bacteria induce inflammation that is differently regulated in healthy and atherosclerotic SMCs.</p>
Original languageEnglish
Pages (from-to)206
JournalSpringerPlus
Volume4
Issue number1
DOIs
Publication statusPublished - 2015
Publication typeA1 Journal article-refereed

Keywords

  • Atherosclerosis
  • Genome wide expression
  • Inflammation
  • Oral bacteria
  • Smooth muscle cells
  • microRNA

Publication forum classification

  • Publication forum level 1

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