Internalization and antigen presentation by mouse dendritic cells of rotavirus VP6 preparations differing in nanostructure

Kirsi Tamminen, Suvi Heinimäki, Stina Gröhn, Vesna Blazevic

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)


Nanoparticles are highly immunogenic due to the multivalent, repetitive antigen expression and direct activation of antigen presenting cells (APCs), key players of adaptive immune responses. Different virus-like particles (VLPs) have been used as display platforms to amplify immune responses to biologically relevant, but poorly immunogenic foreign antigens. A candidate platform based on rotavirus (RV) inner-capsid protein VP6 oligomers, such as nanotubes (T-VP6) and nanospheres (S-VP6), is also considered. Different VP6 nanostructures were compared for internalization and antigen presentation by the APCs. We found, that a lack of a high-order structures, T-VP6 and S-VP6, did not negatively affect VP6 uptake and presentation by murine bone-marrow derived dendritic cells (BMDCs) in vitro. Furthermore, T-VP6 was preferable to norovirus (NoV) VLPs for BMDC internalization resulting in significantly higher VP6-specific immune responses when T-VP6 and NoV VLP pulsed BMDCs were transferred to syngeneic mice. These results support the use of different VP6 nanostructures as foreign antigen delivery platforms.

Original languageEnglish
Pages (from-to)26-31
Number of pages6
Publication statusPublished - Jul 2020
Publication typeA1 Journal article-refereed


  • Animals
  • Antibody Formation
  • Antigen Presentation
  • Antigens, Viral/chemistry
  • Capsid Proteins/chemistry
  • Cells, Cultured
  • Dendritic Cells/immunology
  • Female
  • Mice
  • Mice, Inbred BALB C
  • Nanostructures/chemistry
  • Protein Structure, Tertiary/physiology
  • Protein Transport
  • Recombinant Proteins/chemistry
  • Rotavirus/metabolism
  • Virus Internalization

Publication forum classification

  • Publication forum level 1


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