Label-free Estimation of Sarcomere Orientation from Brightfield Microscopy Images of Induced Pluripotent Stem Cell Derived Cardiomyocyte Nuclei

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    Abstract

    Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) provide a platform for studying disease models and physiological conditions. Sarcomere structure orientation can be used to determine hiPSC-CM culture maturity. However, novel methods are needed for assessing their structure and cellular function. Brightfield microscopy enables continuous label-free measurement of cell cultures in vitro. Here, we propose evaluating sarcomere organization from the morphology and orientation of nuclei in brightfield images. We used publicly available image dataset consisting of brightfield complemented with stained nuclei and α- actinin. We trained a U-Net-based network for segmenting nuclei from brightfield images (IOU of 0.72) and extracted the orientation and aspect ratio of the predicted and stained nuclei. We quantified myofibrillar orientation from α-actinin and determined how it related to nuclei. The analysis revealed correlation between elongated nuclei and the orientation of the surrounding myofibrils. Our results indicate that brightfield data alone can provide estimates of cellular structures without staining. This provides the means to assess structure and maturity from repeated measurements of unstained cells, enabling high-throughput quantification of the in-vitro cardiomyocyte mechanobiology.

    Original languageEnglish
    Title of host publication2022 Computing in Cardiology (CinC)
    PublisherIEEE
    Pages1-4
    ISBN (Electronic)9798350300970
    DOIs
    Publication statusPublished - 2022
    Publication typeA4 Article in conference proceedings
    EventComputing in Cardiology - Tampere Hall, Tampere, Finland
    Duration: 4 Sept 20227 Sept 2022
    Conference number: 49
    https://events.tuni.fi/cinc2022/

    Publication series

    NameComputing in Cardiology
    Volume2022-September
    ISSN (Print)2325-8861
    ISSN (Electronic)2325-887X

    Conference

    ConferenceComputing in Cardiology
    Abbreviated titleCinC 2022
    Country/TerritoryFinland
    CityTampere
    Period4/09/227/09/22
    Internet address

    Publication forum classification

    • Publication forum level 1

    ASJC Scopus subject areas

    • General Computer Science
    • Cardiology and Cardiovascular Medicine

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