Measuring the Mitochondrial Ubiquinone (Q) Pool Redox State in Isolated Respiring Mitochondria

  • Marten Szibor
  • , Estelle Heyne
  • , Carlo Viscomi
  • , Anthony L Moore

    Research output: Chapter in Book/Report/Conference proceedingChapterScientificpeer-review

    3 Citations (Scopus)

    Abstract

    The ubiquinone (Q) pool represents a node in the mitochondrial electron transport chain (ETC) onto which the electrons of all respiratory dehydrogenases converge. The redox state of the Q pool correlates closely with the electron flux through the ETC and is thus a parameter of great metabolic value for both the mitochondrial and cellular metabolism. Here, we describe the simultaneous measurement of respiratory rates of isolated mouse heart mitochondria and the redox state of their Q pool using a custom-made combination of a Clark-type oxygen electrode and a Q electrode.

    Original languageEnglish
    Title of host publicationMitochondria
    Subtitle of host publicationMethods and Protocols
    EditorsNamrata Tomar
    Pages291-299
    Number of pages9
    ISBN (Electronic)978-1-0716-2309-1
    DOIs
    Publication statusPublished - 2022
    Publication typeA3 Book chapter

    Publication series

    NameMethods in molecular biology (Clifton, N.J.)
    PublisherHumana Press
    Volume2497
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

    Keywords

    • Animals
    • Electron Transport
    • Mice
    • Mitochondria, Heart/metabolism
    • Oxidation-Reduction
    • Ubiquinone/metabolism

    Publication forum classification

    • Publication forum level 1

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