Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG'::lux

Jenni Tienaho; Tytti Sarjala; Robert Franzén; Matti Karp

doi:10.1016/j.mimet.2015.08.018

Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG'::lux

Jenni Tienaho^*
, Tytti Sarjala
, Robert Franzén
, Matti Karp

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

12 Citations (Scopus)

Abstract

A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given to obtaining highly reliable and repeatable results.

Original language	English
Article number	4723
Pages (from-to)	78-80
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	118
DOIs	https://doi.org/10.1016/j.mimet.2015.08.018
Publication status	Published - 2015
Publication type	A1 Journal article-refereed

Keywords

Antioxidative activity
Bacterial biosensor
Bioscreening
Microplate technique

Publication forum classification

Publication forum level 1

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2015.08.018

Cite this

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title = "Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG'::lux",

abstract = "A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given to obtaining highly reliable and repeatable results.",

keywords = "Antioxidative activity, Bacterial biosensor, Bioscreening, Microplate technique",

author = "Jenni Tienaho and Tytti Sarjala and Robert Franz{\'e}n and Matti Karp",

year = "2015",

doi = "10.1016/j.mimet.2015.08.018",

language = "English",

volume = "118",

pages = "78--80",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

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TY - JOUR

T1 - Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG'::lux

AU - Tienaho, Jenni

AU - Sarjala, Tytti

AU - Franzén, Robert

AU - Karp, Matti

PY - 2015

Y1 - 2015

N2 - A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given to obtaining highly reliable and repeatable results.

AB - A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given to obtaining highly reliable and repeatable results.

KW - Antioxidative activity

KW - Bacterial biosensor

KW - Bioscreening

KW - Microplate technique

U2 - 10.1016/j.mimet.2015.08.018

DO - 10.1016/j.mimet.2015.08.018

M3 - Article

AN - SCOPUS:84941101607

SN - 0167-7012

VL - 118

SP - 78

EP - 80

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

M1 - 4723

ER -

Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG'::lux

Abstract

Keywords

Publication forum classification

ASJC Scopus subject areas

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