Microbial community assembly and dynamics in Granular, Fixed-Biofilm and planktonic microbiomes valorizing Long-Chain fatty acids at 20 °C

Suniti Singh, Johanna M. Rinta-Kanto, Piet N.L. Lens, Marika Kokko, Jukka Rintala, Vincent O'Flaherty, Umer Zeeshan Ijaz, Gavin Collins

Research output: Contribution to journalArticleScientificpeer-review

2 Downloads (Pure)

Abstract

Distinct microbial assemblages evolve in anaerobic digestion (AD) reactors to drive sequential conversions of organics to methane. The spatio-temporal development of three such assemblages (granules, biofilms, planktonic) derived from the same inoculum was studied in replicated bioreactors treating long-chain fatty acids (LCFA)-rich wastewater at 20 °C at hydraulic retention times (HRTs) of 12–72 h. We found granular, biofilm and planktonic assemblages differentiated by diversity, structure, and assembly mechanisms; demonstrating a spatial compartmentalisation of the microbiomes from the initial community reservoir. Our analysis linked abundant Methanosaeta and Syntrophaceae-affiliated taxa (Syntrophus and uncultured) to their putative, active roles in syntrophic LCFA bioconversion. LCFA loading rates (stearate, palmitate), and HRT, were significant drivers shaping microbial community dynamics and assembly. This study of the archaea and syntrophic bacteria actively valorising LCFAs at short HRTs and 20 °C will help uncover the microbiology underpinning anaerobic bioconversions of fats, oil and grease.

Original languageEnglish
Article number126098
Number of pages11
JournalBioresource Technology
Volume343
Early online date7 Oct 2021
DOIs
Publication statusPublished - 2022
Publication typeA1 Journal article-refereed

Keywords

  • Fats, oils, and grease (FOG)
  • Low-temperature anaerobic digestion
  • Methanogenesis
  • Microbial community assembly
  • Null modelling

Publication forum classification

  • Publication forum level 2

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Fingerprint

Dive into the research topics of 'Microbial community assembly and dynamics in Granular, Fixed-Biofilm and planktonic microbiomes valorizing Long-Chain fatty acids at 20 °C'. Together they form a unique fingerprint.

Cite this