TY - JOUR
T1 - Microbial community assembly and dynamics in Granular, Fixed-Biofilm and planktonic microbiomes valorizing Long-Chain fatty acids at 20 °C
AU - Singh, Suniti
AU - Rinta-Kanto, Johanna M.
AU - Lens, Piet N.L.
AU - Kokko, Marika
AU - Rintala, Jukka
AU - O'Flaherty, Vincent
AU - Ijaz, Umer Zeeshan
AU - Collins, Gavin
N1 - Funding Information:
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie European Joint Doctorate (EJD) in Advanced Biological Waste-To-Energy Technologies (ABWET), under grant agreement No 643071. VOF was supported by the Enterprise Ireland Technology Centres Programme (TC/2014/0016) and Science Foundation Ireland (14/IA/2371 and 16/RC/3889). UZI is further supported by a NERC Independent Research Fellowship (NE/L011956/1) and EPSRC (EP/P029329/1 and EP/V030515/1). GC, and DNA sequencing, was supported by a Science Foundation Ireland Career Development Award (17/CDA/4658).
Funding Information:
This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie European Joint Doctorate (EJD) in Advanced Biological Waste-To-Energy Technologies (ABWET), under grant agreement No 643071. VOF was supported by the Enterprise Ireland Technology Centres Programme (TC/2014/0016) and Science Foundation Ireland (14/IA/2371 and 16/RC/3889). UZI is further supported by a NERC Independent Research Fellowship (NE/L011956/1) and EPSRC (EP/P029329/1 and EP/V030515/1). GC, and DNA sequencing, was supported by a Science Foundation Ireland Career Development Award (17/CDA/4658). The sequencing data from this study are available through the NCBI database under the project accession number accession PRJNA657615.
Publisher Copyright:
© 2021 The Author(s)
PY - 2022
Y1 - 2022
N2 - Distinct microbial assemblages evolve in anaerobic digestion (AD) reactors to drive sequential conversions of organics to methane. The spatio-temporal development of three such assemblages (granules, biofilms, planktonic) derived from the same inoculum was studied in replicated bioreactors treating long-chain fatty acids (LCFA)-rich wastewater at 20 °C at hydraulic retention times (HRTs) of 12–72 h. We found granular, biofilm and planktonic assemblages differentiated by diversity, structure, and assembly mechanisms; demonstrating a spatial compartmentalisation of the microbiomes from the initial community reservoir. Our analysis linked abundant Methanosaeta and Syntrophaceae-affiliated taxa (Syntrophus and uncultured) to their putative, active roles in syntrophic LCFA bioconversion. LCFA loading rates (stearate, palmitate), and HRT, were significant drivers shaping microbial community dynamics and assembly. This study of the archaea and syntrophic bacteria actively valorising LCFAs at short HRTs and 20 °C will help uncover the microbiology underpinning anaerobic bioconversions of fats, oil and grease.
AB - Distinct microbial assemblages evolve in anaerobic digestion (AD) reactors to drive sequential conversions of organics to methane. The spatio-temporal development of three such assemblages (granules, biofilms, planktonic) derived from the same inoculum was studied in replicated bioreactors treating long-chain fatty acids (LCFA)-rich wastewater at 20 °C at hydraulic retention times (HRTs) of 12–72 h. We found granular, biofilm and planktonic assemblages differentiated by diversity, structure, and assembly mechanisms; demonstrating a spatial compartmentalisation of the microbiomes from the initial community reservoir. Our analysis linked abundant Methanosaeta and Syntrophaceae-affiliated taxa (Syntrophus and uncultured) to their putative, active roles in syntrophic LCFA bioconversion. LCFA loading rates (stearate, palmitate), and HRT, were significant drivers shaping microbial community dynamics and assembly. This study of the archaea and syntrophic bacteria actively valorising LCFAs at short HRTs and 20 °C will help uncover the microbiology underpinning anaerobic bioconversions of fats, oil and grease.
KW - Fats, oils, and grease (FOG)
KW - Low-temperature anaerobic digestion
KW - Methanogenesis
KW - Microbial community assembly
KW - Null modelling
U2 - 10.1016/j.biortech.2021.126098
DO - 10.1016/j.biortech.2021.126098
M3 - Article
AN - SCOPUS:85116564379
VL - 343
M1 - 126098
ER -
