Microflow-Based Device for In Vitro and Ex Vivo Drug Permeability Studies

Samu Hemmilä, Marika Ruponen, Elisa Toropainen, Unni Tengvall-Unadike, Arto Urtti, Pasi Kallio

Research output: Contribution to journalArticleScientificpeer-review

Abstract

This paper presents a novel microflow-based concept for studying the permeability of in vitro cell models or ex vivo tissues. Using the proposed concept, we demonstrate how to maintain physiologically relevant test conditions and produce highly reproducible permeability values for a range (31) of drug compounds. The apparent permeability coefficients (Papp) showed excellent correlation (0.89) with the values from experiments performed with a conventional Ussing chamber. Additionally, the microflow-based concept produces notably more concentrated samples than the conventional Ussing chamber-based approach, despite the fact that more than 10 times smaller quantities of test compounds and biological membranes are needed in the microflow-based concept.

Original languageEnglish
Pages (from-to)455–462
Number of pages8
JournalSLAS Technology
Volume25
Issue number5
DOIs
Publication statusPublished - 2020
Publication typeA1 Journal article-refereed

Keywords

  • ex vivo
  • in vitro
  • microfluidics
  • permeability

Publication forum classification

  • Publication forum level 1

ASJC Scopus subject areas

  • Computer Science Applications
  • Medical Laboratory Technology

Fingerprint Dive into the research topics of 'Microflow-Based Device for In Vitro and Ex Vivo Drug Permeability Studies'. Together they form a unique fingerprint.

Cite this