Mild pressure induces rapid accumulation of neutral lipid (triacylglycerol) in Chlorella spp.

Praveen Ramasamy; Bohwa Kim; Jiye Lee; Durairaj Vijayan; Kyubock Lee; Bora Nam; Sang Goo Jeon; Dong Myung Kim; You Kwan Oh

doi:10.1016/j.biortech.2016.09.025

Mild pressure induces rapid accumulation of neutral lipid (triacylglycerol) in Chlorella spp.

Praveen Ramasamy, Bohwa Kim, Jiye Lee, Durairaj Vijayan, Kyubock Lee, Bora Nam, Sang Goo Jeon, Dong Myung Kim, You Kwan Oh

Research output: Contribution to journal › Article › Scientific › peer-review

21 Citations (Scopus)

Abstract

Effective enhancement of neutral lipid (especially triacylglycerol, TAG) content in microalgae is an important issue for commercialization of microalgal biorefineries. Pressure is a key physical factor affecting the morphological, physiological, and biochemical behaviors of organisms. In this paper, we report a new stress-based method for induction of TAG accumulation in microalgae (specifically, Chlorella sp. KR-1 and Ch. sp. AG20150) by very-short-duration application of mild pressure. Pressure treatments of 10–15 bar for 2 h resulted in a considerable, ∼55% improvement of the 10–100 g/L cells’ TAG contents compared with the untreated control. The post-pressure-treatment increase of cytoplasmic TAG granules was further confirmed by transmission electron microscopy (TEM). Notwithstanding the increased TAG content, the total lipid content was not changed by pressurization, implying that pressure stress possibly induces rapid remodeling/transformation of algal lipids rather than de novo biosynthesis of TAG.

Original language	English
Pages (from-to)	661-665
Number of pages	5
Journal	Bioresource Technology
Volume	220
DOIs	https://doi.org/10.1016/j.biortech.2016.09.025
Publication status	Published - 1 Nov 2016
Publication type	A1 Journal article-refereed

Keywords

Induction
Microalgae
Neutral lipid
Pressure stress
Triacylglycerol

Publication forum classification

Publication forum level 2

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Waste Management and Disposal

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10.1016/j.biortech.2016.09.025

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title = "Mild pressure induces rapid accumulation of neutral lipid (triacylglycerol) in Chlorella spp.",

abstract = "Effective enhancement of neutral lipid (especially triacylglycerol, TAG) content in microalgae is an important issue for commercialization of microalgal biorefineries. Pressure is a key physical factor affecting the morphological, physiological, and biochemical behaviors of organisms. In this paper, we report a new stress-based method for induction of TAG accumulation in microalgae (specifically, Chlorella sp. KR-1 and Ch. sp. AG20150) by very-short-duration application of mild pressure. Pressure treatments of 10–15 bar for 2 h resulted in a considerable, ∼55% improvement of the 10–100 g/L cells{\textquoteright} TAG contents compared with the untreated control. The post-pressure-treatment increase of cytoplasmic TAG granules was further confirmed by transmission electron microscopy (TEM). Notwithstanding the increased TAG content, the total lipid content was not changed by pressurization, implying that pressure stress possibly induces rapid remodeling/transformation of algal lipids rather than de novo biosynthesis of TAG.",

keywords = "Induction, Microalgae, Neutral lipid, Pressure stress, Triacylglycerol",

author = "Praveen Ramasamy and Bohwa Kim and Jiye Lee and Durairaj Vijayan and Kyubock Lee and Bora Nam and Jeon, {Sang Goo} and Kim, {Dong Myung} and Oh, {You Kwan}",

year = "2016",

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day = "1",

doi = "10.1016/j.biortech.2016.09.025",

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T1 - Mild pressure induces rapid accumulation of neutral lipid (triacylglycerol) in Chlorella spp.

AU - Ramasamy, Praveen

AU - Kim, Bohwa

AU - Lee, Jiye

AU - Vijayan, Durairaj

AU - Lee, Kyubock

AU - Nam, Bora

AU - Jeon, Sang Goo

AU - Kim, Dong Myung

AU - Oh, You Kwan

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Effective enhancement of neutral lipid (especially triacylglycerol, TAG) content in microalgae is an important issue for commercialization of microalgal biorefineries. Pressure is a key physical factor affecting the morphological, physiological, and biochemical behaviors of organisms. In this paper, we report a new stress-based method for induction of TAG accumulation in microalgae (specifically, Chlorella sp. KR-1 and Ch. sp. AG20150) by very-short-duration application of mild pressure. Pressure treatments of 10–15 bar for 2 h resulted in a considerable, ∼55% improvement of the 10–100 g/L cells’ TAG contents compared with the untreated control. The post-pressure-treatment increase of cytoplasmic TAG granules was further confirmed by transmission electron microscopy (TEM). Notwithstanding the increased TAG content, the total lipid content was not changed by pressurization, implying that pressure stress possibly induces rapid remodeling/transformation of algal lipids rather than de novo biosynthesis of TAG.

AB - Effective enhancement of neutral lipid (especially triacylglycerol, TAG) content in microalgae is an important issue for commercialization of microalgal biorefineries. Pressure is a key physical factor affecting the morphological, physiological, and biochemical behaviors of organisms. In this paper, we report a new stress-based method for induction of TAG accumulation in microalgae (specifically, Chlorella sp. KR-1 and Ch. sp. AG20150) by very-short-duration application of mild pressure. Pressure treatments of 10–15 bar for 2 h resulted in a considerable, ∼55% improvement of the 10–100 g/L cells’ TAG contents compared with the untreated control. The post-pressure-treatment increase of cytoplasmic TAG granules was further confirmed by transmission electron microscopy (TEM). Notwithstanding the increased TAG content, the total lipid content was not changed by pressurization, implying that pressure stress possibly induces rapid remodeling/transformation of algal lipids rather than de novo biosynthesis of TAG.

KW - Induction

KW - Microalgae

KW - Neutral lipid

KW - Pressure stress

KW - Triacylglycerol

U2 - 10.1016/j.biortech.2016.09.025

DO - 10.1016/j.biortech.2016.09.025

M3 - Article

AN - SCOPUS:84989931657

SN - 0960-8524

VL - 220

SP - 661

EP - 665

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Mild pressure induces rapid accumulation of neutral lipid (triacylglycerol) in Chlorella spp.

Abstract

Keywords

Publication forum classification

ASJC Scopus subject areas

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