Miniaturized stimulator for imaging of live cell responses to high frequency mechanical vibration

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

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10 Downloads (Pure)

Abstract

<p> Cellular mechanobiology is highly important for tissue development and disease formation. However, lack of proper tools limit investigation of the cellular responses to different mechanical cues. High frequency (HF) vibration has already been applied in different cellular applications, but the knowledge of the stimulation effect on cells is limited. To meet this challenge, we designed a HF vibration stimulator for combined mechanical manipulation of live cells and high-resolution light-microscopy. Our system utilizes a commercial miniaturized speaker to vibrate a 3D printed sample vehicle horizontally. Technical tests demonstrated excellent performance at lower frequencies (30–60 Hz), enabling even high magnitude (HMHF, G<sub>peak</sub> ≥ 1 G<sub>peak</sub> ) method. Real-time acceleration measurement and light-microscopy both revealed accurately and precisely produced low magnitude (LMHF, G<sub>peak</sub> < 1 G<sub>peak</sub> ) vibrations. With our system, we could observe cellular responses to the LMHF (0.2 G<sub>peak</sub> , 30 Hz) vibration. In this paper, we introduce an inexpensive stimulation platform for the mechanobiology research of different cell applications. </p>
Original languageEnglish
Title of host publicationCMBEBIH 2019 - Proceedings of the International Conference on Medical and Biological Engineering
EditorsAlmir Badnjevic, Lejla Gurbeta Pokvić, Ranko Škrbić
PublisherSpringer Verlag
Pages21-27
Number of pages7
ISBN (Print)978-3-030-17970-0
DOIs
Publication statusPublished - 2019
Publication typeA4 Article in a conference publication
EventInternational Conference on Medical and Biological Engineering in Bosnia and Herzegovina -
Duration: 1 Jan 2019 → …

Publication series

NameIFMBE Proceedings
Volume73
ISSN (Print)1680-0737

Conference

ConferenceInternational Conference on Medical and Biological Engineering in Bosnia and Herzegovina
Period1/01/19 → …

Keywords

  • High frequency vibration
  • Live cell imaging
  • Mechanotransduction

Publication forum classification

  • Publication forum level 1

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