Miniaturized stimulator for imaging of live cell responses to high frequency mechanical vibration

Heidi T. Halonen; Jari A.K. Hyttinen; Teemu O. Ihalainen

doi:10.1007/978-3-030-17971-7_4

Miniaturized stimulator for imaging of live cell responses to high frequency mechanical vibration

Heidi T. Halonen, Jari A.K. Hyttinen, Teemu O. Ihalainen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Scientific › peer-review

3 Citations (Scopus)

44 Downloads (Pure)

Abstract

Cellular mechanobiology is highly important for tissue development and disease formation. However, lack of proper tools limit investigation of the cellular responses to different mechanical cues. High frequency (HF) vibration has already been applied in different cellular applications, but the knowledge of the stimulation effect on cells is limited. To meet this challenge, we designed a HF vibration stimulator for combined mechanical manipulation of live cells and high-resolution light-microscopy. Our system utilizes a commercial miniaturized speaker to vibrate a 3D printed sample vehicle horizontally. Technical tests demonstrated excellent performance at lower frequencies (30–60 Hz), enabling even high magnitude (HMHF, Gpeak ≥ 1 Gpeak ) method. Real-time acceleration measurement and light-microscopy both revealed accurately and precisely produced low magnitude (LMHF, Gpeak < 1 Gpeak ) vibrations. With our system, we could observe cellular responses to the LMHF (0.2 Gpeak , 30 Hz) vibration. In this paper, we introduce an inexpensive stimulation platform for the mechanobiology research of different cell applications.

Original language	English
Title of host publication	CMBEBIH 2019 - Proceedings of the International Conference on Medical and Biological Engineering
Editors	Almir Badnjevic, Lejla Gurbeta Pokvić, Ranko Škrbić
Publisher	Springer Verlag
Pages	21-27
Number of pages	7
ISBN (Print)	978-3-030-17970-0
DOIs	https://doi.org/10.1007/978-3-030-17971-7_4
Publication status	Published - 2019
Publication type	A4 Article in conference proceedings
Event	International Conference on Medical and Biological Engineering in Bosnia and Herzegovina - Duration: 1 Jan 2019 → …

Publication series

Name	IFMBE Proceedings
Volume	73
ISSN (Print)	1680-0737

Conference

Conference	International Conference on Medical and Biological Engineering in Bosnia and Herzegovina
Period	1/01/19 → …

Keywords

High frequency vibration
Live cell imaging
Mechanotransduction

Publication forum classification

Publication forum level 1

Access to Document

10.1007/978-3-030-17971-7_4

Miniaturized stimulator for imaging of live cell responses 2019Accepted author manuscript, 952 KB

http://urn.fi/urn:nbn:fi:tuni-202002061909

Tampere Imaging Facility (TIF) - light microscopy core services
Ihalainen, T. (Manager), Erämies, S. (Contact) & Paloheimo, O. (Contact)
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Cite this

Halonen, H. T., Hyttinen, J. A. K., & Ihalainen, T. O. (2019). Miniaturized stimulator for imaging of live cell responses to high frequency mechanical vibration. In A. Badnjevic, L. Gurbeta Pokvić, & R. Škrbić (Eds.), CMBEBIH 2019 - Proceedings of the International Conference on Medical and Biological Engineering (pp. 21-27). (IFMBE Proceedings; Vol. 73). Springer Verlag. https://doi.org/10.1007/978-3-030-17971-7_4

Halonen, Heidi T. ; Hyttinen, Jari A.K. ; Ihalainen, Teemu O. / Miniaturized stimulator for imaging of live cell responses to high frequency mechanical vibration. CMBEBIH 2019 - Proceedings of the International Conference on Medical and Biological Engineering. editor / Almir Badnjevic ; Lejla Gurbeta Pokvić ; Ranko Škrbić. Springer Verlag, 2019. pp. 21-27 (IFMBE Proceedings).

@inproceedings{c09dee0730ef4a6d9dbfaf6429391e33,

title = "Miniaturized stimulator for imaging of live cell responses to high frequency mechanical vibration",

abstract = " Cellular mechanobiology is highly important for tissue development and disease formation. However, lack of proper tools limit investigation of the cellular responses to different mechanical cues. High frequency (HF) vibration has already been applied in different cellular applications, but the knowledge of the stimulation effect on cells is limited. To meet this challenge, we designed a HF vibration stimulator for combined mechanical manipulation of live cells and high-resolution light-microscopy. Our system utilizes a commercial miniaturized speaker to vibrate a 3D printed sample vehicle horizontally. Technical tests demonstrated excellent performance at lower frequencies (30–60 Hz), enabling even high magnitude (HMHF, Gpeak ≥ 1 Gpeak ) method. Real-time acceleration measurement and light-microscopy both revealed accurately and precisely produced low magnitude (LMHF, Gpeak < 1 Gpeak ) vibrations. With our system, we could observe cellular responses to the LMHF (0.2 Gpeak , 30 Hz) vibration. In this paper, we introduce an inexpensive stimulation platform for the mechanobiology research of different cell applications. ",

keywords = "High frequency vibration, Live cell imaging, Mechanotransduction, High frequency vibration, Live cell imaging, Mechanotransduction",

author = "Halonen, {Heidi T.} and Hyttinen, {Jari A.K.} and Ihalainen, {Teemu O.}",

note = "jufoid=58152, dupl=51710515; International Conference on Medical and Biological Engineering in Bosnia and Herzegovina ; Conference date: 01-01-2019",

year = "2019",

doi = "10.1007/978-3-030-17971-7_4",

language = "English",

isbn = "978-3-030-17970-0",

series = "IFMBE Proceedings",

publisher = "Springer Verlag",

pages = "21--27",

editor = "Almir Badnjevic and {Gurbeta Pokvi{\'c}}, Lejla and Ranko {\v S}krbi{\'c}",

booktitle = "CMBEBIH 2019 - Proceedings of the International Conference on Medical and Biological Engineering",

address = "Germany",

}

Halonen, HT , Hyttinen, JAK & Ihalainen, TO 2019, Miniaturized stimulator for imaging of live cell responses to high frequency mechanical vibration. in A Badnjevic, L Gurbeta Pokvić & R Škrbić (eds), CMBEBIH 2019 - Proceedings of the International Conference on Medical and Biological Engineering. IFMBE Proceedings, vol. 73, Springer Verlag, pp. 21-27, International Conference on Medical and Biological Engineering in Bosnia and Herzegovina, 1/01/19. https://doi.org/10.1007/978-3-030-17971-7_4

Miniaturized stimulator for imaging of live cell responses to high frequency mechanical vibration. / Halonen, Heidi T.; Hyttinen, Jari A.K.; Ihalainen, Teemu O.
CMBEBIH 2019 - Proceedings of the International Conference on Medical and Biological Engineering. ed. / Almir Badnjevic; Lejla Gurbeta Pokvić; Ranko Škrbić. Springer Verlag, 2019. p. 21-27 (IFMBE Proceedings; Vol. 73).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Scientific › peer-review

TY - GEN

T1 - Miniaturized stimulator for imaging of live cell responses to high frequency mechanical vibration

AU - Halonen, Heidi T.

AU - Hyttinen, Jari A.K.

AU - Ihalainen, Teemu O.

N1 - jufoid=58152, dupl=51710515

PY - 2019

Y1 - 2019

N2 - Cellular mechanobiology is highly important for tissue development and disease formation. However, lack of proper tools limit investigation of the cellular responses to different mechanical cues. High frequency (HF) vibration has already been applied in different cellular applications, but the knowledge of the stimulation effect on cells is limited. To meet this challenge, we designed a HF vibration stimulator for combined mechanical manipulation of live cells and high-resolution light-microscopy. Our system utilizes a commercial miniaturized speaker to vibrate a 3D printed sample vehicle horizontally. Technical tests demonstrated excellent performance at lower frequencies (30–60 Hz), enabling even high magnitude (HMHF, Gpeak ≥ 1 Gpeak ) method. Real-time acceleration measurement and light-microscopy both revealed accurately and precisely produced low magnitude (LMHF, Gpeak < 1 Gpeak ) vibrations. With our system, we could observe cellular responses to the LMHF (0.2 Gpeak , 30 Hz) vibration. In this paper, we introduce an inexpensive stimulation platform for the mechanobiology research of different cell applications.

AB - Cellular mechanobiology is highly important for tissue development and disease formation. However, lack of proper tools limit investigation of the cellular responses to different mechanical cues. High frequency (HF) vibration has already been applied in different cellular applications, but the knowledge of the stimulation effect on cells is limited. To meet this challenge, we designed a HF vibration stimulator for combined mechanical manipulation of live cells and high-resolution light-microscopy. Our system utilizes a commercial miniaturized speaker to vibrate a 3D printed sample vehicle horizontally. Technical tests demonstrated excellent performance at lower frequencies (30–60 Hz), enabling even high magnitude (HMHF, Gpeak ≥ 1 Gpeak ) method. Real-time acceleration measurement and light-microscopy both revealed accurately and precisely produced low magnitude (LMHF, Gpeak < 1 Gpeak ) vibrations. With our system, we could observe cellular responses to the LMHF (0.2 Gpeak , 30 Hz) vibration. In this paper, we introduce an inexpensive stimulation platform for the mechanobiology research of different cell applications.

KW - High frequency vibration

KW - Live cell imaging

KW - Mechanotransduction

KW - High frequency vibration

KW - Live cell imaging

KW - Mechanotransduction

U2 - 10.1007/978-3-030-17971-7_4

DO - 10.1007/978-3-030-17971-7_4

M3 - Conference contribution

AN - SCOPUS:85066029834

SN - 978-3-030-17970-0

T3 - IFMBE Proceedings

SP - 21

EP - 27

BT - CMBEBIH 2019 - Proceedings of the International Conference on Medical and Biological Engineering

A2 - Badnjevic, Almir

A2 - Gurbeta Pokvić, Lejla

A2 - Škrbić, Ranko

PB - Springer Verlag

T2 - International Conference on Medical and Biological Engineering in Bosnia and Herzegovina

Y2 - 1 January 2019

ER -

Halonen HT , Hyttinen JAK , Ihalainen TO. Miniaturized stimulator for imaging of live cell responses to high frequency mechanical vibration. In Badnjevic A, Gurbeta Pokvić L, Škrbić R, editors, CMBEBIH 2019 - Proceedings of the International Conference on Medical and Biological Engineering. Springer Verlag. 2019. p. 21-27. (IFMBE Proceedings). doi: 10.1007/978-3-030-17971-7_4

Miniaturized stimulator for imaging of live cell responses to high frequency mechanical vibration

Abstract

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Tampere Imaging Facility (TIF) - light microscopy core services

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