Miniaturized stimulator for imaging of live cell responses to high frequency mechanical vibration

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    44 Downloads (Pure)

    Abstract

    <p> Cellular mechanobiology is highly important for tissue development and disease formation. However, lack of proper tools limit investigation of the cellular responses to different mechanical cues. High frequency (HF) vibration has already been applied in different cellular applications, but the knowledge of the stimulation effect on cells is limited. To meet this challenge, we designed a HF vibration stimulator for combined mechanical manipulation of live cells and high-resolution light-microscopy. Our system utilizes a commercial miniaturized speaker to vibrate a 3D printed sample vehicle horizontally. Technical tests demonstrated excellent performance at lower frequencies (30–60 Hz), enabling even high magnitude (HMHF, G<sub>peak</sub> ≥ 1 G<sub>peak</sub> ) method. Real-time acceleration measurement and light-microscopy both revealed accurately and precisely produced low magnitude (LMHF, G<sub>peak</sub> < 1 G<sub>peak</sub> ) vibrations. With our system, we could observe cellular responses to the LMHF (0.2 G<sub>peak</sub> , 30 Hz) vibration. In this paper, we introduce an inexpensive stimulation platform for the mechanobiology research of different cell applications. </p>
    Original languageEnglish
    Title of host publicationCMBEBIH 2019 - Proceedings of the International Conference on Medical and Biological Engineering
    EditorsAlmir Badnjevic, Lejla Gurbeta Pokvić, Ranko Škrbić
    PublisherSpringer Verlag
    Pages21-27
    Number of pages7
    ISBN (Print)978-3-030-17970-0
    DOIs
    Publication statusPublished - 2019
    Publication typeA4 Article in conference proceedings
    EventInternational Conference on Medical and Biological Engineering in Bosnia and Herzegovina -
    Duration: 1 Jan 2019 → …

    Publication series

    NameIFMBE Proceedings
    Volume73
    ISSN (Print)1680-0737

    Conference

    ConferenceInternational Conference on Medical and Biological Engineering in Bosnia and Herzegovina
    Period1/01/19 → …

    Keywords

    • High frequency vibration
    • Live cell imaging
    • Mechanotransduction

    Publication forum classification

    • Publication forum level 1

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