Mitochondrial temperature homeostasis resists external metabolic stresses

Mügen Terzioglu, Kristo Veeroja, Toni Montonen, Teemu O. Ihalainen, Tiina S. Salminen, Paule Bénit, Pierre Rustin, Young Tae Chang, Takeharu Nagai, Howard T. Jacobs

    Research output: Contribution to journalArticleScientificpeer-review

    2 Citations (Scopus)
    12 Downloads (Pure)

    Abstract

    Based on studies with a fluorescent reporter dye, Mito Thermo Yellow (MTY), and the genetically encoded gTEMP ratiometric fluorescent temperature indicator targeted to mitochondria, the temperature of active mitochondria in four mammalian and one insect cell line was estimated to be up to 15°C above that of the external environment to which the cells were exposed. High mitochondrial temperature was maintained in the face of a variety of metabolic stresses, including substrate starvation or modification, decreased ATP demand due to inhibition of cytosolic protein synthesis, inhibition of the mitochondrial adenine nucleotide transporter and, if an auxiliary pathway for electron transfer was available via the alternative oxidase, even respiratory poisons acting down-stream of oxidative phosphorylation (OXPHOS) complex I. We propose that the high temperature of active mitochondria is an inescapable consequence of the biochemistry of OXPHOS and is homeo-statically maintained as a primary feature of mitochondrial metabolism.

    Original languageEnglish
    Article numberRP89232
    JournaleLIFE
    Volume12
    DOIs
    Publication statusPublished - 2023
    Publication typeA1 Journal article-refereed

    Publication forum classification

    • Publication forum level 2

    ASJC Scopus subject areas

    • General Neuroscience
    • General Biochemistry,Genetics and Molecular Biology
    • General Immunology and Microbiology

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