Novel bioaugmentation strategy boosted with biochar to alleviate ammonia toxicity in continuous biomethanation

Yixin Yan; Miao Yan; Giulia Ravenni; Irini Angelidaki; Dafang Fu; Ioannis A. Fotidis

doi:10.1016/j.biortech.2021.126146

Novel bioaugmentation strategy boosted with biochar to alleviate ammonia toxicity in continuous biomethanation

Yixin Yan
, Miao Yan
, Giulia Ravenni
, Irini Angelidaki
, Dafang Fu
, Ioannis A. Fotidis^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

32 Citations (Scopus)

12 Downloads (Pure)

Abstract

This study investigated for the first time if ammonia tolerant methanogenic consortia can be stored in gel (biogel) and used in a later time on-demand as bioaugmentation inocula, to efficiently relieve ammonia inhibition in continuous biomethanation systems. Moreover, wood biochar was assessed as a potential enhancer of the novel biogel bioaugmentation process. Three thermophilic (55 °C), continuous stirred-tank reactors (R_Bgel, R_Char and R_Mix), operated at 4.5 g NH₄⁺-N L^-1 were exposed to biogel, biochar and mixture of biogel and biochar, respectively, while a fourth reactor (R_Ctrl) was used as control. The results showed that the methane production yields of R_Mix, R_Char and R_Bgel increased by 28.6%, 20.2% and 10.7%, respectively compared to R_Ctrl. The highest methane yield was achieved by the synergistic interaction between biogel and biochar. Additionally, biogel stimulated a rapid recovery of Methanoculleus thermophilus sp. and syntrophic acetate oxidising bacteria populations.

Original language	English
Article number	126146
Number of pages	8
Journal	Bioresource Technology
Volume	343
Early online date	18 Oct 2021
DOIs	https://doi.org/10.1016/j.biortech.2021.126146
Publication status	Published - 2022
Publication type	A1 Journal article-refereed

Funding

Yixin Yan appreciate the financial support from China Scholarship Council. Ioannis A. Fotidis acknowledges the financial support of National Natural Science Foundation of China , International Cooperation and Exchange Program “LyoCH 4 -Development of novel lyophilized bioaugmentation inocula to alleviate ammonia toxicity in anaerobic reactors” ( 5185041512 ).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Ammonia tolerance
Bioremediation
Interspecies interaction
Methanoculleus thermophilus
Methanogenesis

Publication forum classification

Publication forum level 2

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

Access to Document

10.1016/j.biortech.2021.126146Licence: CC BY

Novel bioaugmentation strategyFinal published version, 4.06 MBLicence: CC BY

https://urn.fi/URN:NBN:fi:tuni-202111028096Licence: CC BY

Cite this

@article{fe47d4a02ce5464d84166f7b600dbdda,

title = "Novel bioaugmentation strategy boosted with biochar to alleviate ammonia toxicity in continuous biomethanation",

abstract = "This study investigated for the first time if ammonia tolerant methanogenic consortia can be stored in gel (biogel) and used in a later time on-demand as bioaugmentation inocula, to efficiently relieve ammonia inhibition in continuous biomethanation systems. Moreover, wood biochar was assessed as a potential enhancer of the novel biogel bioaugmentation process. Three thermophilic (55 °C), continuous stirred-tank reactors (RBgel, RChar and RMix), operated at 4.5 g NH4+-N L-1 were exposed to biogel, biochar and mixture of biogel and biochar, respectively, while a fourth reactor (RCtrl) was used as control. The results showed that the methane production yields of RMix, RChar and RBgel increased by 28.6\%, 20.2\% and 10.7\%, respectively compared to RCtrl. The highest methane yield was achieved by the synergistic interaction between biogel and biochar. Additionally, biogel stimulated a rapid recovery of Methanoculleus thermophilus sp. and syntrophic acetate oxidising bacteria populations.",

keywords = "Ammonia tolerance, Bioremediation, Interspecies interaction, Methanoculleus thermophilus, Methanogenesis",

author = "Yixin Yan and Miao Yan and Giulia Ravenni and Irini Angelidaki and Dafang Fu and Fotidis, \{Ioannis A.\}",

note = "Funding Information: Yixin Yan appreciate the financial support from China Scholarship Council. Ioannis A. Fotidis acknowledges the financial support of National Natural Science Foundation of China , International Cooperation and Exchange Program “LyoCH 4 -Development of novel lyophilized bioaugmentation inocula to alleviate ammonia toxicity in anaerobic reactors” ( 5185041512 ). Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2022",

doi = "10.1016/j.biortech.2021.126146",

language = "English",

volume = "343",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Novel bioaugmentation strategy boosted with biochar to alleviate ammonia toxicity in continuous biomethanation

AU - Yan, Yixin

AU - Yan, Miao

AU - Ravenni, Giulia

AU - Angelidaki, Irini

AU - Fu, Dafang

AU - Fotidis, Ioannis A.

N1 - Funding Information: Yixin Yan appreciate the financial support from China Scholarship Council. Ioannis A. Fotidis acknowledges the financial support of National Natural Science Foundation of China , International Cooperation and Exchange Program “LyoCH 4 -Development of novel lyophilized bioaugmentation inocula to alleviate ammonia toxicity in anaerobic reactors” ( 5185041512 ). Publisher Copyright: © 2021 The Author(s)

PY - 2022

Y1 - 2022

N2 - This study investigated for the first time if ammonia tolerant methanogenic consortia can be stored in gel (biogel) and used in a later time on-demand as bioaugmentation inocula, to efficiently relieve ammonia inhibition in continuous biomethanation systems. Moreover, wood biochar was assessed as a potential enhancer of the novel biogel bioaugmentation process. Three thermophilic (55 °C), continuous stirred-tank reactors (RBgel, RChar and RMix), operated at 4.5 g NH4+-N L-1 were exposed to biogel, biochar and mixture of biogel and biochar, respectively, while a fourth reactor (RCtrl) was used as control. The results showed that the methane production yields of RMix, RChar and RBgel increased by 28.6%, 20.2% and 10.7%, respectively compared to RCtrl. The highest methane yield was achieved by the synergistic interaction between biogel and biochar. Additionally, biogel stimulated a rapid recovery of Methanoculleus thermophilus sp. and syntrophic acetate oxidising bacteria populations.

AB - This study investigated for the first time if ammonia tolerant methanogenic consortia can be stored in gel (biogel) and used in a later time on-demand as bioaugmentation inocula, to efficiently relieve ammonia inhibition in continuous biomethanation systems. Moreover, wood biochar was assessed as a potential enhancer of the novel biogel bioaugmentation process. Three thermophilic (55 °C), continuous stirred-tank reactors (RBgel, RChar and RMix), operated at 4.5 g NH4+-N L-1 were exposed to biogel, biochar and mixture of biogel and biochar, respectively, while a fourth reactor (RCtrl) was used as control. The results showed that the methane production yields of RMix, RChar and RBgel increased by 28.6%, 20.2% and 10.7%, respectively compared to RCtrl. The highest methane yield was achieved by the synergistic interaction between biogel and biochar. Additionally, biogel stimulated a rapid recovery of Methanoculleus thermophilus sp. and syntrophic acetate oxidising bacteria populations.

KW - Ammonia tolerance

KW - Bioremediation

KW - Interspecies interaction

KW - Methanoculleus thermophilus

KW - Methanogenesis

U2 - 10.1016/j.biortech.2021.126146

DO - 10.1016/j.biortech.2021.126146

M3 - Article

AN - SCOPUS:85117403302

SN - 0960-8524

VL - 343

JO - Bioresource Technology

JF - Bioresource Technology

M1 - 126146

ER -

Novel bioaugmentation strategy boosted with biochar to alleviate ammonia toxicity in continuous biomethanation

Abstract

Funding

UN SDGs

Keywords

Publication forum classification

ASJC Scopus subject areas

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