Online Monitoring of Volatile Products of Bioprocess Using Differential Ion Mobility─A Proof of Concept with 1-Undecene

Milla Salmela; Meri Mäkelä; Changshuo Liu; Markus Karjalainen; Anton Kontunen; Suvi Santala; Antti Roine; Ville Santala

doi:10.1021/acs.iecr.3c04188

Online Monitoring of Volatile Products of Bioprocess Using Differential Ion Mobility─A Proof of Concept with 1-Undecene

Milla Salmela
, Meri Mäkelä
, Changshuo Liu
, Markus Karjalainen
, Anton Kontunen
, Suvi Santala
, Antti Roine
, Ville Santala^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

Advancements in synthetic biology have driven significant progress in chemical production using engineered microbes, yet challenges persist in characterizing strains and identifying optimal process conditions. This study introduces differential mobility spectrometry (DMS), a technique that ionizes and separates gaseous molecules in high and low electric fields, as a novel approach to monitor bioprocesses. We applied DMS to a bioprocess unit producing alpha-olefin 1-undecene, an industrially relevant platform chemical, through genetically engineered Acinetobacter baylyi ADP1 cells. DMS effectively captured the dynamic nature of 1-undecene production, demonstrating its potential as a nontargeted method for real-time bioprocess studies and optimizations.

Original language	English
Pages (from-to)	7481-7484
Journal	Industrial and Engineering Chemistry Research
Volume	63
Issue number	16
DOIs	https://doi.org/10.1021/acs.iecr.3c04188
Publication status	Published - 24 Apr 2024
Publication type	A1 Journal article-refereed

Funding

The authors gratefully acknowledge Business Finland for funding (SynbioPro project). S.S. and V.S. would like to thank the Novo Nordisk Foundation (grants NNF21OC0067758 and NNF22OC0079579). We would like to thank Osmo Anttalainen for important input on ion chemistry and ionization.

Funders	Funder number
Business Finland
Novo Nordisk Fonden	NNF22OC0079579, NNF21OC0067758

Publication forum classification

Publication forum level 1

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering
Industrial and Manufacturing Engineering

Access to Document

10.1021/acs.iecr.3c04188Licence: CC BY

Online-monitoring-of-volatile-products-of-bioprocess-using-differential-ion-mobility-a-proof-ofFinal published version, 2.59 MBLicence: CC BY

https://urn.fi/URN:NBN:fi:tuni-202406207339Licence: CC BY

Synthetic biology
Santala, V. (Contact) & Santala, S. (Contact)
Materials Science and Environmental Engineering
Facility/equipment: Research infrastructure

Cite this

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title = "Online Monitoring of Volatile Products of Bioprocess Using Differential Ion Mobility─A Proof of Concept with 1-Undecene",

abstract = "Advancements in synthetic biology have driven significant progress in chemical production using engineered microbes, yet challenges persist in characterizing strains and identifying optimal process conditions. This study introduces differential mobility spectrometry (DMS), a technique that ionizes and separates gaseous molecules in high and low electric fields, as a novel approach to monitor bioprocesses. We applied DMS to a bioprocess unit producing alpha-olefin 1-undecene, an industrially relevant platform chemical, through genetically engineered Acinetobacter baylyi ADP1 cells. DMS effectively captured the dynamic nature of 1-undecene production, demonstrating its potential as a nontargeted method for real-time bioprocess studies and optimizations.",

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doi = "10.1021/acs.iecr.3c04188",

language = "English",

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AU - Salmela, Milla

AU - Mäkelä, Meri

AU - Liu, Changshuo

AU - Karjalainen, Markus

AU - Kontunen, Anton

AU - Santala, Suvi

AU - Roine, Antti

AU - Santala, Ville

PY - 2024/4/24

Y1 - 2024/4/24

N2 - Advancements in synthetic biology have driven significant progress in chemical production using engineered microbes, yet challenges persist in characterizing strains and identifying optimal process conditions. This study introduces differential mobility spectrometry (DMS), a technique that ionizes and separates gaseous molecules in high and low electric fields, as a novel approach to monitor bioprocesses. We applied DMS to a bioprocess unit producing alpha-olefin 1-undecene, an industrially relevant platform chemical, through genetically engineered Acinetobacter baylyi ADP1 cells. DMS effectively captured the dynamic nature of 1-undecene production, demonstrating its potential as a nontargeted method for real-time bioprocess studies and optimizations.

AB - Advancements in synthetic biology have driven significant progress in chemical production using engineered microbes, yet challenges persist in characterizing strains and identifying optimal process conditions. This study introduces differential mobility spectrometry (DMS), a technique that ionizes and separates gaseous molecules in high and low electric fields, as a novel approach to monitor bioprocesses. We applied DMS to a bioprocess unit producing alpha-olefin 1-undecene, an industrially relevant platform chemical, through genetically engineered Acinetobacter baylyi ADP1 cells. DMS effectively captured the dynamic nature of 1-undecene production, demonstrating its potential as a nontargeted method for real-time bioprocess studies and optimizations.

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ER -

Online Monitoring of Volatile Products of Bioprocess Using Differential Ion Mobility─A Proof of Concept with 1-Undecene

Abstract

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Synthetic biology

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