Optical non-contact pH measurement in cell culture with sterilizable, modular parts

Dhanesh Kattipparambil Rajan, Mimmi Patrikoski, Jarmo Verho, Jyrki Sivula, Heimo Ihalainen, Susanna Miettinen, Jukka Lekkala, R Kattipparambil

    Research output: Contribution to journalArticleScientificpeer-review

    14 Citations (Scopus)
    3 Downloads (Pure)

    Abstract

    A non-contact real time pH measurement using fully modular optical parts is described for phenol-red medium cell cultures. The modular parts can be sterilized, and once the measurement is started at the beginning of culture, no recalibration or maintenance is needed till the end of the culture. Measurements can be carried out without any special manual attention. The modular assembly of LED and sensor cassettes is unique, robust, reusable and reproducible. pH is measured in an intact closed flow system, without wasting any culture medium. A special pump encapsulation enables the system to be effortlessly functional in extremely humid incubator environments. This avoids lengthy sample tubings in and out of the incubator, associated large temperature changes and CO2 buffering issues. A new correction model to compensate errors caused e.g. by biolayers in spectrometric pH measurement is put-forward, which improves the accuracy of pH estimation significantly. The method provides resolution down to 0.1 pH unit in physiological pH range with mean absolute error 0.02.

    Original languageEnglish
    Pages (from-to)755-761
    Number of pages6
    JournalTalanta
    Volume161
    DOIs
    Publication statusPublished - 2016
    Publication typeA1 Journal article-refereed

    Keywords

    • Cell culture non-contact pH measurement
    • Cell culture optical pH measurement
    • Cell culture pH measurement
    • Long term pH measurement
    • Phenol red medium pH measurement

    Publication forum classification

    • Publication forum level 1

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