Platform for controlling cellular environment

Antti Mäki, Dhanesh Kattipparambil Rajan, Joose Kreutzer, Anne Skogberg, Mari Pekkanen-Mattila, Jarmo Antero Verho, Tomi Ryynänen, Hannu Välimäki, Antti Ahola, Jari Hyttinen, Katriina Aalto-Setälä, Jukka Lekkala, Pasi Kallio

    Research output: Other conference contributionAbstractScientific


    We present here a platform for controlling cellular microenvironments and functionalities. The aim is to provide physiologically relevant culture conditions and to control and regulate the functions of cells.

    The system includes modules for environment measurement and control [1-3], mechanical cell stimulation [4-5], and cell imaging and analysis [6] together with bicompatible, flexible, and cell-friendly materials as presented in Figure 1. The set-up of the system can be changed to meet the requirements of the physiological study. For example, the system can be used for a long-term stem cell culturing outside an incubator and to study the behavior of cells to different environment conditions.

    We used beating cardiomyocytes to demonstrate the capability of the platform in two experiments, where we studied the relationship between the beating rate and 1) culture temperature [2], 2) hypoxia conditions [3].

    [1] J. Kreutzer et al., “Cell Culture Chamber with Gas Supply for Prolonged Recording of Human Neuronal Cells on Microelectrode Array,” J. Neurosci. Methods, vol. 280, pp. 27–35, 2017.
    [2] A.-J. Mäki et al., “A Portable Microscale Cell Culture System with Indirect Temperature Control,” manuscripts submitted for publication.
    [3] H. Välimäki et al., “Fluorimetric oxygen sensor with an efficient optical read-out for in vitro cell models,” Sensors Actuators B Chem., vol. 249, pp. 738–746, 2017.
    [4] CytoSpectre, a software tool for the analysis of orientation and wavelength distributions from micrographs:
    [5] J. Kreutzer et al., “Pneumatic cell stretching system for cardiac differentiation and culture,” Med. Eng. Phys., vol. 36, no. 4, pp. 496–501, 2014.
    [6] D. Rajan et al., “A portable live-cell imaging system with an invert-upright-convertible architecture and a mini-bioreactor for long-term simultaneous cell imaging, chemical sensing and electrophysiological recording,” IEEE Access, in press.
    Original languageEnglish
    Number of pages1
    Publication statusPublished - 24 May 2018
    Publication typeNot Eligible

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