Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy

Joose Kreutzer; Marlitt Viehrig; Antti-Juhana Maki; Pasi Kallio; Rolle Rahikainen; Vesa Hytönen

doi:10.1109/marss.2017.8001929

Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy

Joose Kreutzer
, Marlitt Viehrig
, Antti-Juhana Maki
, Pasi Kallio
, Rolle Rahikainen
, Vesa Hytönen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Scientific › peer-review

3 Citations (Scopus)

53 Downloads (Pure)

Abstract

In this study, we demonstrate the functionality and usability of a compact, pneumatically actuated, elastomeric stimulation device for mechanobiological studies. The soft mechatronic device enables high-resolution live-cell confocal fluorescent imaging during equiaxial stretching. Several single cells can be tracked and imaged repeatedly after stretching periods. For demonstration, we provide image based analysis of dynamic change of the cell body and the nucleus area and actin fiber orientation during mechanical stimulation of mouse embryonic fibroblast (MEF) cells. Additionally, we present the characteristics of the device utilizing computational simulations and experimental validation using a particle tracking method for strain field analysis.

Original language	English
Title of host publication	International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings
Editors	S Haliyo, S Martel, S. Fatikow
Publisher	IEEE
ISBN (Electronic)	978-1-5386-0346-8
DOIs	https://doi.org/10.1109/marss.2017.8001929
Publication status	Published - 2017
Publication type	A4 Article in conference proceedings
Event	International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS) - Duration: 1 Jan 2017 → …

Conference

Conference	International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS)
Period	1/01/17 → …

Keywords

High-resolution imaging
Mechanical stimulation
Mouse embryonic fibroblasts
PDMS
Particle tracking
Pneumatic actuation
Strain field analysis

Publication forum classification

Publication forum level 1

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10.1109/marss.2017.8001929

MARSS2017 - KreutzerAccepted author manuscript, 1.94 MB

http://urn.fi/urn:nbn:fi:tty-201712202442

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Kreutzer, J., Viehrig, M., Maki, A.-J., Kallio, P., Rahikainen, R., & Hytönen, V. (2017). Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy. In S. Haliyo, S. Martel, & S. Fatikow (Eds.), International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings IEEE. https://doi.org/10.1109/marss.2017.8001929

@inproceedings{265b6293de11403cb5e9f5a099250e40,

title = "Pneumatically actuated elastomeric device for simultaneous mechanobiological studies \& live-cell fluorescent microscopy",

abstract = "In this study, we demonstrate the functionality and usability of a compact, pneumatically actuated, elastomeric stimulation device for mechanobiological studies. The soft mechatronic device enables high-resolution live-cell confocal fluorescent imaging during equiaxial stretching. Several single cells can be tracked and imaged repeatedly after stretching periods. For demonstration, we provide image based analysis of dynamic change of the cell body and the nucleus area and actin fiber orientation during mechanical stimulation of mouse embryonic fibroblast (MEF) cells. Additionally, we present the characteristics of the device utilizing computational simulations and experimental validation using a particle tracking method for strain field analysis.",

keywords = "High-resolution imaging, Mechanical stimulation, Mouse embryonic fibroblasts, PDMS, Particle tracking, Pneumatic actuation, Strain field analysis, High-resolution imaging, Mechanical stimulation, Mouse embryonic fibroblasts, PDMS, Particle tracking, Pneumatic actuation, Strain field analysis",

author = "Joose Kreutzer and Marlitt Viehrig and Antti-Juhana Maki and Pasi Kallio and Rolle Rahikainen and Vesa Hyt{\"o}nen",

note = "INT=tut-bmt,{"}Viehrig, Marlitt{"}; International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS) ; Conference date: 01-01-2017",

year = "2017",

doi = "10.1109/marss.2017.8001929",

language = "English",

editor = "S Haliyo and S Martel and S. Fatikow",

booktitle = "International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings",

publisher = "IEEE",

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Kreutzer, J, Viehrig, M, Maki, A-J, Kallio, P , Rahikainen, R & Hytönen, V 2017, Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy. in S Haliyo, S Martel & S Fatikow (eds), International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings. IEEE, International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS), 1/01/17. https://doi.org/10.1109/marss.2017.8001929

Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy. / Kreutzer, Joose; Viehrig, Marlitt; Maki, Antti-Juhana et al.
International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings. ed. / S Haliyo; S Martel; S. Fatikow. IEEE, 2017.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Scientific › peer-review

TY - GEN

T1 - Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy

AU - Kreutzer, Joose

AU - Viehrig, Marlitt

AU - Maki, Antti-Juhana

AU - Kallio, Pasi

AU - Rahikainen, Rolle

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N2 - In this study, we demonstrate the functionality and usability of a compact, pneumatically actuated, elastomeric stimulation device for mechanobiological studies. The soft mechatronic device enables high-resolution live-cell confocal fluorescent imaging during equiaxial stretching. Several single cells can be tracked and imaged repeatedly after stretching periods. For demonstration, we provide image based analysis of dynamic change of the cell body and the nucleus area and actin fiber orientation during mechanical stimulation of mouse embryonic fibroblast (MEF) cells. Additionally, we present the characteristics of the device utilizing computational simulations and experimental validation using a particle tracking method for strain field analysis.

AB - In this study, we demonstrate the functionality and usability of a compact, pneumatically actuated, elastomeric stimulation device for mechanobiological studies. The soft mechatronic device enables high-resolution live-cell confocal fluorescent imaging during equiaxial stretching. Several single cells can be tracked and imaged repeatedly after stretching periods. For demonstration, we provide image based analysis of dynamic change of the cell body and the nucleus area and actin fiber orientation during mechanical stimulation of mouse embryonic fibroblast (MEF) cells. Additionally, we present the characteristics of the device utilizing computational simulations and experimental validation using a particle tracking method for strain field analysis.

KW - High-resolution imaging

KW - Mechanical stimulation

KW - Mouse embryonic fibroblasts

KW - PDMS

KW - Particle tracking

KW - Pneumatic actuation

KW - Strain field analysis

KW - High-resolution imaging

KW - Mechanical stimulation

KW - Mouse embryonic fibroblasts

KW - PDMS

KW - Particle tracking

KW - Pneumatic actuation

KW - Strain field analysis

U2 - 10.1109/marss.2017.8001929

DO - 10.1109/marss.2017.8001929

M3 - Conference contribution

AN - SCOPUS:85030222654

BT - International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings

A2 - Haliyo, S

A2 - Martel, S

A2 - Fatikow, S.

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ER -

Kreutzer J, Viehrig M, Maki AJ, Kallio P , Rahikainen R , Hytönen V. Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy. In Haliyo S, Martel S, Fatikow S, editors, International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings. IEEE. 2017 doi: 10.1109/marss.2017.8001929

Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy

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