Rapid detection of antimicrobial resistance markers with Allplex™ Entero-DR assay directly from positive blood culture bottles

Paavo Hannus; Kati Räisänen; Jari J. Martelin

doi:10.1007/s10096-020-04082-5

Rapid detection of antimicrobial resistance markers with Allplex™ Entero-DR assay directly from positive blood culture bottles

Paavo Hannus, Kati Räisänen, Jari J. Martelin

Department of Clinical Chemistry

Research output: Contribution to journal › Article › Scientific › peer-review

1 Citation (Scopus)

Abstract

A method for rapid detection of one extended-spectrum β-lactamase (ESBL) and five carbapenemase-encoding genes as well as vancomycin resistance markers directly from blood cultures using the Allplex™ Entero-DR assay (Seegene, Seoul, South Korea) is presented. Altogether 28 previously well-characterized resistant Gram-negative bacilli and Enterococcus spp., and 142 clinical blood cultures containing Gram-negative bacilli or Gram-positive cocci were analyzed. The method had 100% sensitivity and specificity for detecting bla_OXA-48-like,bla_KPC, bla_VIM, bla_IMP, bla_NDM, bla_CTX-M, vanA, and vanB. The lowest detectable amount of viable cells in blood culture samples were 5.39·10⁴ CFU/mL, 6.66·10⁴ CFU/mL, 5.13·10³ CFU/mL, 6.09·10⁴ CFU/mL, 6.66·10⁴ CFU/mL, 6.66·10⁴ CFU/mL, 3.12·10⁴ CFU/mL, and 5.34·10⁴ CFU/mL for the bla_KPC, bla_OXA-48-like, bla_VIM, bla_IMP, bla_NDM, bla_CTX-M, vanA, and vanB, respectively. The results were available within 90 min from signal positive blood cultures, as no separate DNA extraction steps were needed, and the assay showed no interference from blood or culture media used allowing reliable and simplified detection of the resistance markers.

Original language	English
Pages (from-to)	801-806
Number of pages	6
Journal	European Journal of Clinical Microbiology and Infectious Diseases
Volume	40
Issue number	4
DOIs	https://doi.org/10.1007/s10096-020-04082-5
Publication status	Published - Apr 2021
Publication type	A1 Journal article-refereed

Keywords

Bacteraemia
Blood culture
Carbapenemases
CPE
Enterobacterales
Enterococcus spp
ESBL
Sepsis
Vancomycin resistance

Publication forum classification

Publication forum level 1

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/s10096-020-04082-5

Cite this

@article{9abb2f4f9f0b45d2993f20234b12fa70,

title = "Rapid detection of antimicrobial resistance markers with Allplex{\texttrademark} Entero-DR assay directly from positive blood culture bottles",

abstract = "A method for rapid detection of one extended-spectrum β-lactamase (ESBL) and five carbapenemase-encoding genes as well as vancomycin resistance markers directly from blood cultures using the Allplex{\texttrademark} Entero-DR assay (Seegene, Seoul, South Korea) is presented. Altogether 28 previously well-characterized resistant Gram-negative bacilli and Enterococcus spp., and 142 clinical blood cultures containing Gram-negative bacilli or Gram-positive cocci were analyzed. The method had 100% sensitivity and specificity for detecting blaOXA-48-like,blaKPC, blaVIM, blaIMP, blaNDM, blaCTX-M, vanA, and vanB. The lowest detectable amount of viable cells in blood culture samples were 5.39·104 CFU/mL, 6.66·104 CFU/mL, 5.13·103 CFU/mL, 6.09·104 CFU/mL, 6.66·104 CFU/mL, 6.66·104 CFU/mL, 3.12·104 CFU/mL, and 5.34·104 CFU/mL for the blaKPC, blaOXA-48-like, blaVIM, blaIMP, blaNDM, blaCTX-M, vanA, and vanB, respectively. The results were available within 90 min from signal positive blood cultures, as no separate DNA extraction steps were needed, and the assay showed no interference from blood or culture media used allowing reliable and simplified detection of the resistance markers.",

keywords = "Bacteraemia, Blood culture, Carbapenemases, CPE, Enterobacterales, Enterococcus spp, ESBL, Sepsis, Vancomycin resistance",

author = "Paavo Hannus and Kati R{\"a}is{\"a}nen and Martelin, {Jari J.}",

note = "Funding Information: Allplex Entero-DR test kits were kindly provided by Triolab Ltd (Turku, Finland) for this study. Publisher Copyright: {\textcopyright} 2020, Springer-Verlag GmbH Germany, part of Springer Nature.",

year = "2021",

month = apr,

doi = "10.1007/s10096-020-04082-5",

language = "English",

volume = "40",

pages = "801--806",

number = "4",

}

Rapid detection of antimicrobial resistance markers with Allplex™ Entero-DR assay directly from positive blood culture bottles. / Hannus, Paavo; Räisänen, Kati; Martelin, Jari J.
In: European Journal of Clinical Microbiology and Infectious Diseases, Vol. 40, No. 4, 04.2021, p. 801-806.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Rapid detection of antimicrobial resistance markers with Allplex™ Entero-DR assay directly from positive blood culture bottles

AU - Hannus, Paavo

AU - Räisänen, Kati

AU - Martelin, Jari J.

N1 - Funding Information: Allplex Entero-DR test kits were kindly provided by Triolab Ltd (Turku, Finland) for this study. Publisher Copyright: © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2021/4

Y1 - 2021/4

N2 - A method for rapid detection of one extended-spectrum β-lactamase (ESBL) and five carbapenemase-encoding genes as well as vancomycin resistance markers directly from blood cultures using the Allplex™ Entero-DR assay (Seegene, Seoul, South Korea) is presented. Altogether 28 previously well-characterized resistant Gram-negative bacilli and Enterococcus spp., and 142 clinical blood cultures containing Gram-negative bacilli or Gram-positive cocci were analyzed. The method had 100% sensitivity and specificity for detecting blaOXA-48-like,blaKPC, blaVIM, blaIMP, blaNDM, blaCTX-M, vanA, and vanB. The lowest detectable amount of viable cells in blood culture samples were 5.39·104 CFU/mL, 6.66·104 CFU/mL, 5.13·103 CFU/mL, 6.09·104 CFU/mL, 6.66·104 CFU/mL, 6.66·104 CFU/mL, 3.12·104 CFU/mL, and 5.34·104 CFU/mL for the blaKPC, blaOXA-48-like, blaVIM, blaIMP, blaNDM, blaCTX-M, vanA, and vanB, respectively. The results were available within 90 min from signal positive blood cultures, as no separate DNA extraction steps were needed, and the assay showed no interference from blood or culture media used allowing reliable and simplified detection of the resistance markers.

AB - A method for rapid detection of one extended-spectrum β-lactamase (ESBL) and five carbapenemase-encoding genes as well as vancomycin resistance markers directly from blood cultures using the Allplex™ Entero-DR assay (Seegene, Seoul, South Korea) is presented. Altogether 28 previously well-characterized resistant Gram-negative bacilli and Enterococcus spp., and 142 clinical blood cultures containing Gram-negative bacilli or Gram-positive cocci were analyzed. The method had 100% sensitivity and specificity for detecting blaOXA-48-like,blaKPC, blaVIM, blaIMP, blaNDM, blaCTX-M, vanA, and vanB. The lowest detectable amount of viable cells in blood culture samples were 5.39·104 CFU/mL, 6.66·104 CFU/mL, 5.13·103 CFU/mL, 6.09·104 CFU/mL, 6.66·104 CFU/mL, 6.66·104 CFU/mL, 3.12·104 CFU/mL, and 5.34·104 CFU/mL for the blaKPC, blaOXA-48-like, blaVIM, blaIMP, blaNDM, blaCTX-M, vanA, and vanB, respectively. The results were available within 90 min from signal positive blood cultures, as no separate DNA extraction steps were needed, and the assay showed no interference from blood or culture media used allowing reliable and simplified detection of the resistance markers.

KW - Bacteraemia

KW - Blood culture

KW - Carbapenemases

KW - CPE

KW - Enterobacterales

KW - Enterococcus spp

KW - ESBL

KW - Sepsis

KW - Vancomycin resistance

U2 - 10.1007/s10096-020-04082-5

DO - 10.1007/s10096-020-04082-5

M3 - Article

C2 - 33099709

AN - SCOPUS:85093942053

SN - 0934-9723

VL - 40

SP - 801

EP - 806

JO - European Journal of Clinical Microbiology and Infectious Diseases

JF - European Journal of Clinical Microbiology and Infectious Diseases

IS - 4

ER -

Rapid detection of antimicrobial resistance markers with Allplex™ Entero-DR assay directly from positive blood culture bottles

Abstract

Keywords

Publication forum classification

ASJC Scopus subject areas

UN SDGs

Access to Document

Fingerprint

Cite this