Rapid enumeration of virus-like particles in drinking water samples using SYBR green I-staining

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Abstract

We studied the suitability of SYBR green I-staining for determining total counts of virus-like particles and bacteria in drinking water. Low background fluorescence and lack of unspecific staining made drinking water samples an excellent matrix for SYBR green I-staining. Direct microscopic count method is a rapid and economical tool for assessing the total number of virus-like particles in aquatic samples, compared to culture-dependent or molecular biology methods. We applied this method to show the efficiency of a large-scale drinking water purification process in the removal of virus-like particles and bacteria from lake water. © 2004 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)2614-2618
Number of pages5
JournalWater Research
Volume38
Issue number10
DOIs
Publication statusPublished - May 2004
Externally publishedYes
Publication typeA1 Journal article-refereed

Keywords

  • Bacteria
  • Bacteriophage
  • Drinking water
  • SYBR green I
  • Viruses

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