Regulation of endogenous human NPFF2 receptor by neuropeptide FF in SK-N-MC neuroblastoma cell line

Minna-Liisa Änkö, Pertti Panula

Research output: Contribution to journalArticleScientificpeer-review

21 Citations (Scopus)


Neuropeptide FF has many functions both in the CNS and periphery. Two G protein-coupled receptors (NPFF1 and NPFF2 receptors) have been identified for neuropeptide FF. The expression analysis of the peptide and receptors, together with pharmacological and physiological data, imply that NPFF2 receptor would be the primary receptor for neuropeptide FF. Here, we report for the first time a cell line endogenously expressing hNPFF2 receptor. These SK-N-MC neuroblastoma cells also express neuropeptide FF. We used the cells to investigate the hNPFF2 receptor function. The pertussis toxin-sensitive inhibition of adenylate cyclase activity upon receptor activation indicated coupling to Gi/o proteins. Upon agonist exposure, the receptors were internalized and the mitogen-activated protein kinase cascade was activated. Upon neuropeptide FF treatment, the actin cytoskeleton was reorganized in the cells. The expression of hNPFF2 receptor mRNA was up-regulated by neuropeptide FF. Concomitant with the receptor mRNA, the receptor protein expression was increased. The homologous regulation of hNPFF2 receptor correlates with our previous results in vivo showing that during inflammation, the up-regulation of neuropeptide FF mRNA precedes that of NPFF2 receptor. The regulation of hNPFF2 receptor by NPFF could also be important in the periphery where neuropeptide FF has been suggested to function as a hormone.

Original languageEnglish
Pages (from-to)573-584
Number of pages12
JournalJournal of Neurochemistry
Issue number2
Publication statusPublished - Jan 2006
Externally publishedYes
Publication typeA1 Journal article-refereed


  • Actin stress fibre
  • GPCR
  • Homologous gene regulation
  • Neuropeptide FF
  • NPFF2 receptor
  • SK-N-MC

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


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