@inproceedings{5a40df42e1ea4af7a2259898d4bff4ec,
title = "Reproducible preparation method of hydrogels for cell culture applications – Case study with spermidine crosslinked gellan gum",
abstract = "Hydrogels are promising materials to culture cells in 3D environment. Their mechanical properties are decisive, as cells understand the stiffness of their surroundings. Herein, a method is presented to produce ionically crosslinked hydrogel matrices. A reproducible method is needed, because conventional methods cause inconsistent properties. The investigated material is gellan gum, crosslinked with the bioamine spermidine. Samples were prepared with the more conventional {\textquoteleft}pipetting{\textquoteright} technique and with self-developed {\textquoteleft}uniform mixing{\textquoteright} technique. The two preparation techniques are described in detail and the obtained hydrogels are compared. The mechanical properties are analyzed with compression testing. The obtained results show that samples by the so-called {\textquoteleft}uniform mixing{\textquoteright} method have more uniform dimensions and higher compression modulus. A preliminary stability test in cell culture medium was also carried out.",
keywords = "3D cell culture, Compression modulus, Gellan gum, Hydrogel, Spermidine, 3D cell culture, Compression modulus, Gellan gum, Hydrogel, Spermidine",
author = "C. Gering and Koivisto, {J. T.} and Parraga, {J. E.} and M. Kellom{\"a}ki",
note = "jufoid=58152; Joint Conference of the European Medical and Biological Engineering Conference (EMBEC) and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC) ; Conference date: 01-01-2018",
year = "2018",
doi = "10.1007/978-981-10-5122-7_203",
language = "English",
isbn = "978-981-10-5121-0",
series = "IFMBE Proceedings",
publisher = "Springer Verlag",
pages = "811--814",
editor = "Hannu Eskola",
booktitle = "EMBEC and NBC 2017 - Joint Conference of the European Medical and Biological Engineering Conference EMBEC 2017 and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2017",
address = "Germany",
}