Retrieval of the conductivity spectrum of tissues in vitro with novel multimodal tomography

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Objective: Imaging of tissue engineered three-dimensional (3D) specimens is challenging due to their thickness. We propose a novel multimodal imaging technique to obtain multi-physical 3D images and the electrical conductivity spectrum of tissue engineered specimens in vitro. Approach: We combine simultaneous recording of rotational multifrequency electrical impedance tomography (R-mfEIT) with optical projection tomography (OPT). Structural details of the specimen provided by OPT are used here as geometrical priors for R-mfEIT. Main results: This data fusion enables accurate retrieval of the conductivity spectrum of the specimen. We demonstrate experimentally the feasibility of the proposed technique using a potato phantom, adipose and liver tissues, and stem cells in biomaterial spheroids. The results indicate that the proposed technique can distinguish between viable and dead tissues and detect the presence of stem cells. Significance: This technique is expected to become a valuable tool for monitoring tissue engineered specimens' growth and viability in vitro.

Original languageEnglish
Article number205016
JournalPhysics in Medicine and Biology
Volume66
Issue number20
DOIs
Publication statusPublished - 2021
Publication typeA1 Journal article-refereed

Keywords

  • electrical impedance tomography (EIT)
  • hybrid imaging
  • limited angle tomography
  • optical projection tomography (OPT)
  • rotational multifrequency electrical impedance tomography (R-mfEIT)
  • tissue engineering

Publication forum classification

  • Publication forum level 2

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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