TY - JOUR
T1 - RNA-protein interactions in vivo
T2 - Global gets specific
AU - Änkö, Minna-Liisa
AU - Neugebauer, Karla M.
N1 - Funding Information:
We thank Michael Hiller and members of our laboratory for helpful discussions and apologize to those authors whose work we could not cite owing to space limitations. We are grateful for support from the Max Planck Society (to K.M.N.), the Deutsche Forschungsgemeinschaft (NE909/3-1 to K.M.N.), the Helsingin Sanomain Foundation, the Sigrid Juselius Foundation and the Jane and Aatos Erkko Foundation (to M-L.Ä.).
PY - 2012/7
Y1 - 2012/7
N2 - RNA-binding proteins (RBPs) impact every process in the cell; they act as splicing and polyadenylation factors, transport and localization factors, stabilizers and destabilizers, modifiers, and chaperones. RNA-binding capacity can be attributed to numerous protein domains that bind a limited repertoire of short RNA sequences. How is specificity achieved in cells? Here we focus on recent advances in determining the RNA-binding properties of proteins in vivo and compare these to in vitro determinations, highlighting insights into how endogenous RNA molecules are recognized and regulated. We also discuss the crucial contribution of structural determinations for understanding RNA-binding specificity and mechanisms.
AB - RNA-binding proteins (RBPs) impact every process in the cell; they act as splicing and polyadenylation factors, transport and localization factors, stabilizers and destabilizers, modifiers, and chaperones. RNA-binding capacity can be attributed to numerous protein domains that bind a limited repertoire of short RNA sequences. How is specificity achieved in cells? Here we focus on recent advances in determining the RNA-binding properties of proteins in vivo and compare these to in vitro determinations, highlighting insights into how endogenous RNA molecules are recognized and regulated. We also discuss the crucial contribution of structural determinations for understanding RNA-binding specificity and mechanisms.
U2 - 10.1016/j.tibs.2012.02.005
DO - 10.1016/j.tibs.2012.02.005
M3 - Review Article
C2 - 22425269
AN - SCOPUS:84862883971
SN - 0968-0004
VL - 37
SP - 255
EP - 262
JO - TRENDS IN BIOCHEMICAL SCIENCES
JF - TRENDS IN BIOCHEMICAL SCIENCES
IS - 7
ER -