TY - JOUR
T1 - Role of HLA-DQ typing and antitissue transglutaminase antibody titres in diagnosing coeliac disease among Sudanese children with type 1 diabetes mellitus
AU - Ibaid, Ibtihag
AU - Hussien, Mohammed
AU - Kaukinen, Katri
AU - Sabir, Omayma
AU - Elmekki, Miskelyemen
AU - Musa, Azza
AU - Abdelhadi, Nasreldein
AU - El Hussein, Abdel Rahim
AU - Saavalainen, Päivi
N1 - Funding Information:
Funding This study was supported by the Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan (Grant Number 2007/152).
Funding Information:
7Department of Statistics and Documentation, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan 8Faculty of Pharmacy, The National Ribat University, Khartoum, Sudan, Khartoum, Sudan 9Deparment of Virology, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan 10Translational Immunology Research Program, Department of Clinical and Medical Genetics, University of Helsinki, Helsinki, Finland Contributors II and MH carried out the methodology and drafted the manuscript. OS provided the samples and data collection. AM conducted the statistical analysis. NA, KK, AREH and PS contributed to the conception and design of the study and revised the manuscript. II acting as guarator. All authors have read and approved the final version of this manuscript.
Publisher Copyright:
© Author(s) (or their employer(s)) 2022.
PY - 2022/1/24
Y1 - 2022/1/24
N2 - Objective The aim of the study was to determine the prevalence of coeliac disease (CD) and to recognise Human leukocyte antigen (HLA)-associated hereditary susceptibility to Sudanese CD patients with type 1 diabetes mellitus (DM1). Design Antitissue transglutaminase IgA (anti-TG IgA) was measured in the serum of 373 children affected with DM1 aged 1-19-year old and in 100 serum samples from non-diabetic control children. Histological examination was performed in 19 children seropositive for anti-TG IgA (17 DMI and 2 controls). Additionally, PCR-based analysis of Major histocompatibility complex, class II, DQ beta 1 (HLA-DQB1) genotyping was implemented in three study population groups as follows: group 1 (n=25) (+ve DM1 and +ve CD), group 2 (n=63) (-ve DM1 and +ve CD) and control group 3 (n=2) (+ve CD). Results Twenty-six Sudanese children with DM1 out of 373 (6.97%) were seropositive for anti-TG IgA. Duodenal biopsy revealed Marsh 2 and 3 in 13 out of 17 (76.47%) seropositive anti-TG IgA patients with DM1. Significant association (p<0.05) was detected between the level of anti-TG IgA autoantibodies (IU/mL) and Marsh stage. HLA DQ2 and DQ8 were found in 88% (22/25) and 8% (2/25) of examined patients with CD with DM1, respectively. Conclusions Anti-TG IgA titre of greater than 10 times upper limit of normal (≥10× ULN) can be useful for detecting CD in children with type 1 diabetes without duodenal biopsy. HLA testing in children with DM1 appears to provide little added benefit given the high prevalence (96%) of HLA DQ2/DQ8 in children with DM1.
AB - Objective The aim of the study was to determine the prevalence of coeliac disease (CD) and to recognise Human leukocyte antigen (HLA)-associated hereditary susceptibility to Sudanese CD patients with type 1 diabetes mellitus (DM1). Design Antitissue transglutaminase IgA (anti-TG IgA) was measured in the serum of 373 children affected with DM1 aged 1-19-year old and in 100 serum samples from non-diabetic control children. Histological examination was performed in 19 children seropositive for anti-TG IgA (17 DMI and 2 controls). Additionally, PCR-based analysis of Major histocompatibility complex, class II, DQ beta 1 (HLA-DQB1) genotyping was implemented in three study population groups as follows: group 1 (n=25) (+ve DM1 and +ve CD), group 2 (n=63) (-ve DM1 and +ve CD) and control group 3 (n=2) (+ve CD). Results Twenty-six Sudanese children with DM1 out of 373 (6.97%) were seropositive for anti-TG IgA. Duodenal biopsy revealed Marsh 2 and 3 in 13 out of 17 (76.47%) seropositive anti-TG IgA patients with DM1. Significant association (p<0.05) was detected between the level of anti-TG IgA autoantibodies (IU/mL) and Marsh stage. HLA DQ2 and DQ8 were found in 88% (22/25) and 8% (2/25) of examined patients with CD with DM1, respectively. Conclusions Anti-TG IgA titre of greater than 10 times upper limit of normal (≥10× ULN) can be useful for detecting CD in children with type 1 diabetes without duodenal biopsy. HLA testing in children with DM1 appears to provide little added benefit given the high prevalence (96%) of HLA DQ2/DQ8 in children with DM1.
KW - celiac disease
KW - nutrition
KW - paediatric gastroenterology
U2 - 10.1136/bmjgast-2021-000735
DO - 10.1136/bmjgast-2021-000735
M3 - Article
AN - SCOPUS:85124072747
SN - 2054-4774
VL - 9
JO - BMJ Open Gastroenterology
JF - BMJ Open Gastroenterology
IS - 1
M1 - e000735
ER -
