Saline Accelerates Oxime Reaction with Aldehyde and Keto Substrates at Physiological pH

Shujiang Wang, Ganesh N. Nawale, Sandeep Kadekar, Oommen Podiyan Oommen, Naresh K. Jena, Sudip Chakraborty, Jöns Hilborn, Oommen P. Varghese

    Research output: Contribution to journalArticleScientificpeer-review

    11 Citations (Scopus)
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    Abstract

    We have discovered a simple and versatile reaction condition for oxime mediated bioconjugation reaction that could be adapted for both aldehyde and keto substrates. We found that saline accelerated the oxime kinetics in a concentration-dependent manner under physiological conditions. The reaction mechanism is validated by computational studies, and the versatility of the reaction is demonstrated by cell-surface labeling experiments. Saline offers an efficient and non-toxic catalytic option for performing the bioorthogonal-coupling reaction of biomolecules at the physiological pH. This saline mediated bioconjugation reaction represents the most biofriendly, mild and versatile approach for conjugating sensitive biomolecules and does not require any extensive purification step.
    Original languageEnglish
    Article number2193
    JournalScientific Reports
    Volume8
    Issue number1
    DOIs
    Publication statusPublished - 1 Feb 2018
    Publication typeA1 Journal article-refereed

    Publication forum classification

    • Publication forum level 2

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