Serum proteomics of mother-infant dyads carrying HLA-conferred type 1 diabetes risk

Santosh D. Bhosale; Robert Moulder; Tomi Suomi; Terhi Ruohtula; Jarno Honkanen; Suvi M. Virtanen; Jorma Ilonen; Laura L. Elo; Mikael Knip; Riitta Lahesmaa

doi:10.1016/j.isci.2024.110048

Serum proteomics of mother-infant dyads carrying HLA-conferred type 1 diabetes risk

Santosh D. Bhosale
, Robert Moulder
, Tomi Suomi
, Terhi Ruohtula
, Jarno Honkanen
, Suvi M. Virtanen
, Jorma Ilonen
, Laura L. Elo
, Mikael Knip
, Riitta Lahesmaa^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

In-utero and dietary factors make important contributions toward health and development in early childhood. In this respect, serum proteomics of maturing infants can provide insights into studies of childhood diseases, which together with perinatal proteomes could reveal further biological perspectives. Accordingly, to determine differences between feeding groups and changes in infancy, serum proteomics analyses of mother-infant dyads with HLA-conferred susceptibility to type 1 diabetes (n = 22), weaned to either an extensively hydrolyzed or regular cow's milk formula, were made. The LC-MS/MS analyses included samples from the beginning of third trimester, the time of delivery, 3 months postpartum, cord blood, and samples from the infants at 3, 6, 9, and 12 months. Correlations between ranked protein intensities were detected within the dyads, together with perinatal and age-related changes. Comparison with intestinal permeability data revealed a number of significant correlations, which could merit further consideration in this context.

Original language	English
Article number	110048
Journal	iScience
Volume	27
Issue number	6
DOIs	https://doi.org/10.1016/j.isci.2024.110048
Publication status	Published - 21 Jun 2024
Publication type	A1 Journal article-refereed

Funding

The work was financially supported by Juvenile Diabetes Research Foundation Ltd , the Academy of Finland , the Finnish Diabetes Foundation , the Sigrid Jus\u00E9lius Foundation , and InFLAMES Flagship Program. R.L. received funding from the Academy of Finland (grants 292335 , 294337 , 319280 , 31444 , 319280 , 329277 , and 331790 ), Business Finland and by grants from the JDRF, Sigrid Jus\u00E9lius Foundation, Jane and Aatos Erkko Foundation , Finnish Diabetes Foundation, and the Finnish Cancer Foundation . L.E. reports grants from the European Research Council ERC ( 677943 ), Academy of Finland ( 296801 , 310561 , 314443 , 329278 , 335434 , and 335611 ), and Sigrid Jus\u00E9lius Foundation during the conduct of the study. R.L. and M.K. were supported by the Academy of Finland, AoF, Centre of Excellence in Molecular Systems Immunology and Physiology Research (2012\u20132017, grants 250114 and 292482 ). M.K. also received support from the Sigrid Jus\u00E9lius Foundation, Helsinki University Hospital Research Funds and the Liv and H\u00E4lsa Fund. Research at Turku Bioscience Center (L.E. and R.L.) was supported by University of Turku Graduate School (UTUGS), Biocenter Finland , ELIXIR Finland , and the InFLAMES Flagship Program of the Academy of Finland (decision number: 337530). The work was financially supported by Juvenile Diabetes Research Foundation Ltd, the Academy of Finland, the Finnish Diabetes Foundation, the Sigrid Jus\u00E9lius Foundation, and InFLAMES Flagship Program. R.L. received funding from the Academy of Finland (grants 292335, 294337, 319280, 31444, 319280, 329277, and 331790), Business Finland and by grants from the JDRF, Sigrid Jus\u00E9lius Foundation, Jane and Aatos Erkko Foundation, Finnish Diabetes Foundation, and the Finnish Cancer Foundation. L.E. reports grants from the European Research Council ERC (677943), Academy of Finland (296801, 310561, 314443, 329278, 335434, and 335611), and Sigrid Jus\u00E9lius Foundation during the conduct of the study. R.L. and M.K. were supported by the Academy of Finland, AoF, Centre of Excellence in Molecular Systems Immunology and Physiology Research (2012\u20132017, grants 250114 and 292482). M.K. also received support from the Sigrid Jus\u00E9lius Foundation, Helsinki University Hospital Research Funds and the Liv and H\u00E4lsa Fund. Research at Turku Bioscience Center (L.E. and R.L.) was supported by University of Turku Graduate School (UTUGS), Biocenter Finland, ELIXIR Finland, and the InFLAMES Flagship Program of the Academy of Finland (decision number: 337530). The work presented in this paper was conducted at Turku Bioscience Center (University of Turku and \u00C5bo Akademi University). The proteomics analysis were conducted at the Turku Bioscience Proteomics core, which is supported by Biocenter Finland. Conceptualization, M.K. and R.L.; methodology, S.D.B. R.M. and T.S.; investigation, S.D.B. R.M. T.S. T.R. J.H. S.V. and J.I.; writing \u2013 original draft, R.M. and S.D.B.; writing \u2013 review and editing, all authors reviewed and revised the manuscript and approved the submitted version; funding acquisition, M.K. R.L. and L.E.; resources, M.K. and S.V.; supervision, R.L. M.K. and L.E. The authors declare no competing interests.

Funders	Funder number
Juvenile Diabetes Research Foundation International
Jane ja Aatos Erkon Säätiö
Liv and Hälsa Fund
Finnish Diabetes Foundation
Business Finland
ELIXIR Finland
Syöpäjärjestöt
University of Turku and Åbo Akademi University
Juvenile Diabetes Research Foundation United States of America
Helsingin ja Uudenmaan sairaanhoitopiiri
Biocenter Finland
Sigrid Juséliuksen Säätiö
Turun yliopiston tutkijakoulu
Turku Bioscience Center
H2020 European Research Council	677943, 250114, 296801, 329278, 335434, 292482, 310561, 335611, 314443
Strategic Research Council at the Research Council of Finland	331790, 31444, 337530, 294337, 292335, 329277, 319280

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

biochemistry
human metabolism
human physiology
immunology
omics
proteomics

Publication forum classification

Publication forum level 1

ASJC Scopus subject areas

General

Access to Document

10.1016/j.isci.2024.110048Licence: CC BY-NC

1-s2.0-S2589004224012732-mainFinal published version, 3 MBLicence: CC BY-NC

https://urn.fi/URN:NBN:fi:tuni-202406117102Licence: CC BY-NC

Cite this

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title = "Serum proteomics of mother-infant dyads carrying HLA-conferred type 1 diabetes risk",

abstract = "In-utero and dietary factors make important contributions toward health and development in early childhood. In this respect, serum proteomics of maturing infants can provide insights into studies of childhood diseases, which together with perinatal proteomes could reveal further biological perspectives. Accordingly, to determine differences between feeding groups and changes in infancy, serum proteomics analyses of mother-infant dyads with HLA-conferred susceptibility to type 1 diabetes (n = 22), weaned to either an extensively hydrolyzed or regular cow's milk formula, were made. The LC-MS/MS analyses included samples from the beginning of third trimester, the time of delivery, 3 months postpartum, cord blood, and samples from the infants at 3, 6, 9, and 12 months. Correlations between ranked protein intensities were detected within the dyads, together with perinatal and age-related changes. Comparison with intestinal permeability data revealed a number of significant correlations, which could merit further consideration in this context.",

keywords = "biochemistry, human metabolism, human physiology, immunology, omics, proteomics",

author = "Bhosale, \{Santosh D.\} and Robert Moulder and Tomi Suomi and Terhi Ruohtula and Jarno Honkanen and Virtanen, \{Suvi M.\} and Jorma Ilonen and Elo, \{Laura L.\} and Mikael Knip and Riitta Lahesmaa",

note = "Publisher Copyright: {\textcopyright} 2024 The Author(s)",

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doi = "10.1016/j.isci.2024.110048",

language = "English",

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T1 - Serum proteomics of mother-infant dyads carrying HLA-conferred type 1 diabetes risk

AU - Bhosale, Santosh D.

AU - Moulder, Robert

AU - Suomi, Tomi

AU - Ruohtula, Terhi

AU - Honkanen, Jarno

AU - Virtanen, Suvi M.

AU - Ilonen, Jorma

AU - Elo, Laura L.

AU - Knip, Mikael

AU - Lahesmaa, Riitta

PY - 2024/6/21

Y1 - 2024/6/21

N2 - In-utero and dietary factors make important contributions toward health and development in early childhood. In this respect, serum proteomics of maturing infants can provide insights into studies of childhood diseases, which together with perinatal proteomes could reveal further biological perspectives. Accordingly, to determine differences between feeding groups and changes in infancy, serum proteomics analyses of mother-infant dyads with HLA-conferred susceptibility to type 1 diabetes (n = 22), weaned to either an extensively hydrolyzed or regular cow's milk formula, were made. The LC-MS/MS analyses included samples from the beginning of third trimester, the time of delivery, 3 months postpartum, cord blood, and samples from the infants at 3, 6, 9, and 12 months. Correlations between ranked protein intensities were detected within the dyads, together with perinatal and age-related changes. Comparison with intestinal permeability data revealed a number of significant correlations, which could merit further consideration in this context.

AB - In-utero and dietary factors make important contributions toward health and development in early childhood. In this respect, serum proteomics of maturing infants can provide insights into studies of childhood diseases, which together with perinatal proteomes could reveal further biological perspectives. Accordingly, to determine differences between feeding groups and changes in infancy, serum proteomics analyses of mother-infant dyads with HLA-conferred susceptibility to type 1 diabetes (n = 22), weaned to either an extensively hydrolyzed or regular cow's milk formula, were made. The LC-MS/MS analyses included samples from the beginning of third trimester, the time of delivery, 3 months postpartum, cord blood, and samples from the infants at 3, 6, 9, and 12 months. Correlations between ranked protein intensities were detected within the dyads, together with perinatal and age-related changes. Comparison with intestinal permeability data revealed a number of significant correlations, which could merit further consideration in this context.

KW - biochemistry

KW - human metabolism

KW - human physiology

KW - immunology

KW - omics

KW - proteomics

U2 - 10.1016/j.isci.2024.110048

DO - 10.1016/j.isci.2024.110048

M3 - Article

AN - SCOPUS:85194503199

SN - 2589-0042

VL - 27

JO - iScience

JF - iScience

IS - 6

M1 - 110048

ER -

Serum proteomics of mother-infant dyads carrying HLA-conferred type 1 diabetes risk

Abstract

Funding

UN SDGs

Keywords

Publication forum classification

ASJC Scopus subject areas

Access to Document

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Cite this