Spatial and temporal variation in denitrification and in the denitrifier community in a boreal lake

Antti Rissanen; Marja Tiirola; Anne Ojala

doi:10.3354/ame01506

Spatial and temporal variation in denitrification and in the denitrifier community in a boreal lake

Antti Rissanen
, Marja Tiirola
, Anne Ojala

Research output: Contribution to journal › Article › Scientific › peer-review

36 Citations (Scopus)

Abstract

We investigated the spatial and temporal variation in denitrification rates (isotope-pairing technique, IPT) and in the denitrifier community (examination of gene nirK by denaturinggradient gel electrophoresis [DGGE] of microbial DNA) in the sediments of a boreal, clear-water, eutrophic lake using samples collected from shallow littoral, deep littoral and shallow profundal sediments during early summer, mid-summer, autumn and winter. The measured denitrification rates (44 to 613 mu mol N m(-2) d(-1)) are among the lowest ever reported from lacustrine sediments. Denitrification rates varied both spatially and temporally, being highest in the profundal zone during midsummer and in the littoral zones during winter. Correlation analyses indicated that these variations were due to variations in the concentrations of nitrate and oxygen in the water overlying the sediment. The structure of the denitrifier community was temporally extremely stable and differed only slightly between the sites. Distance-based linear model (DISTLM) analysis indicated that the observed variation was probably due mainly to variations in the content of organic matter, and in the porosity, of the sediment. The structure of the denitrifier community and the denitrifying activities were uncoupled.

Original language	English
Pages (from-to)	27-40
Number of pages	14
Journal	Aquatic Microbial Ecology
Volume	64
Issue number	1
DOIs	https://doi.org/10.3354/ame01506
Publication status	Published - 2011
Publication type	A1 Journal article-refereed

Funding

We acknowledge the Lammi Biological Station, University of Helsinki, where all the analyses as well as denitrification incubations were performed. This study was funded by the Finnish Cultural Foundation, Maa- ja Vesitekniikan tuki ry and Maj and Tor Nessling Foundation. We thank D. Nizzoli and I. Ahlgren for providing nitrate concentration data. We also thank R. Jones, S. Hietanen and H. Jantti for helpful comments on our manuscript. Furthermore, we thank the 3 anonymous reviewers for their valuable and constructive comments.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

Keywords

Denitrification
Lake
Sediment
nirK
Bacterial diversity
Boreal
ANAEROBIC AMMONIUM OXIDATION
LENGTH-POLYMORPHISM ANALYSIS
16S RIBOSOMAL-RNA
CONTINENTAL-MARGIN SEDIMENTS
GULF-OF-MEXICO
BACTERIAL COMMUNITIES
SEASONAL-VARIATION
MARINE-SEDIMENTS
WATER COLUMN
FRESH-WATER

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title = "Spatial and temporal variation in denitrification and in the denitrifier community in a boreal lake",

abstract = "We investigated the spatial and temporal variation in denitrification rates (isotope-pairing technique, IPT) and in the denitrifier community (examination of gene nirK by denaturinggradient gel electrophoresis [DGGE] of microbial DNA) in the sediments of a boreal, clear-water, eutrophic lake using samples collected from shallow littoral, deep littoral and shallow profundal sediments during early summer, mid-summer, autumn and winter. The measured denitrification rates (44 to 613 mu mol N m(-2) d(-1)) are among the lowest ever reported from lacustrine sediments. Denitrification rates varied both spatially and temporally, being highest in the profundal zone during midsummer and in the littoral zones during winter. Correlation analyses indicated that these variations were due to variations in the concentrations of nitrate and oxygen in the water overlying the sediment. The structure of the denitrifier community was temporally extremely stable and differed only slightly between the sites. Distance-based linear model (DISTLM) analysis indicated that the observed variation was probably due mainly to variations in the content of organic matter, and in the porosity, of the sediment. The structure of the denitrifier community and the denitrifying activities were uncoupled.",

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doi = "10.3354/ame01506",

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AU - Rissanen, Antti

AU - Tiirola, Marja

AU - Ojala, Anne

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N2 - We investigated the spatial and temporal variation in denitrification rates (isotope-pairing technique, IPT) and in the denitrifier community (examination of gene nirK by denaturinggradient gel electrophoresis [DGGE] of microbial DNA) in the sediments of a boreal, clear-water, eutrophic lake using samples collected from shallow littoral, deep littoral and shallow profundal sediments during early summer, mid-summer, autumn and winter. The measured denitrification rates (44 to 613 mu mol N m(-2) d(-1)) are among the lowest ever reported from lacustrine sediments. Denitrification rates varied both spatially and temporally, being highest in the profundal zone during midsummer and in the littoral zones during winter. Correlation analyses indicated that these variations were due to variations in the concentrations of nitrate and oxygen in the water overlying the sediment. The structure of the denitrifier community was temporally extremely stable and differed only slightly between the sites. Distance-based linear model (DISTLM) analysis indicated that the observed variation was probably due mainly to variations in the content of organic matter, and in the porosity, of the sediment. The structure of the denitrifier community and the denitrifying activities were uncoupled.

AB - We investigated the spatial and temporal variation in denitrification rates (isotope-pairing technique, IPT) and in the denitrifier community (examination of gene nirK by denaturinggradient gel electrophoresis [DGGE] of microbial DNA) in the sediments of a boreal, clear-water, eutrophic lake using samples collected from shallow littoral, deep littoral and shallow profundal sediments during early summer, mid-summer, autumn and winter. The measured denitrification rates (44 to 613 mu mol N m(-2) d(-1)) are among the lowest ever reported from lacustrine sediments. Denitrification rates varied both spatially and temporally, being highest in the profundal zone during midsummer and in the littoral zones during winter. Correlation analyses indicated that these variations were due to variations in the concentrations of nitrate and oxygen in the water overlying the sediment. The structure of the denitrifier community was temporally extremely stable and differed only slightly between the sites. Distance-based linear model (DISTLM) analysis indicated that the observed variation was probably due mainly to variations in the content of organic matter, and in the porosity, of the sediment. The structure of the denitrifier community and the denitrifying activities were uncoupled.

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KW - LENGTH-POLYMORPHISM ANALYSIS

KW - 16S RIBOSOMAL-RNA

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KW - GULF-OF-MEXICO

KW - BACTERIAL COMMUNITIES

KW - SEASONAL-VARIATION

KW - MARINE-SEDIMENTS

KW - WATER COLUMN

KW - FRESH-WATER

U2 - 10.3354/ame01506

DO - 10.3354/ame01506

M3 - Article

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SP - 27

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JO - Aquatic Microbial Ecology

JF - Aquatic Microbial Ecology

IS - 1

ER -

Spatial and temporal variation in denitrification and in the denitrifier community in a boreal lake

Abstract

Funding

UN SDGs

Keywords

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