Structured PDMS chambers for enhanced human neuronal cell activity on MEA platforms

Joose Kreutzer, Laura Ylä-Outinen, Paula Kärnä, Tiina Kaarela, Jarno Mikkonen, Heli Skottman, Susanna Narkilahti, Pasi Kallio

    Research output: Contribution to journalArticleScientificpeer-review

    22 Citations (Scopus)
    127 Downloads (Pure)

    Abstract

    Structured poly(dimethylsiloxane) (PDMS) chambers were designed and fabricated to enhance the signaling of human embryonic stem cell (hESC) - derived neuronal networks on microelectrode array (MEA) platforms. The structured PDMS chambers enable cell seeding on restricted areas and thus, reduce the amount of needed coating materials and cells. In addition, the neuronal cells formed spontaneously active networks faster in the structured PDMS chambers than in control chambers. In the PDMS chambers, the neuronal networks were more active and able to develop their signaling into organized signal trains faster than control cultures. The PDMS chamber design enables much more repeatable analysis and rapid growth of functional neuronal network in vitro. Moreover, due to its easy and cheap fabrication process, new configurations can be easily fabricated based on investigator requirements.
    Original languageEnglish
    Pages (from-to)1-10
    Number of pages10
    JournalJournal of Bionic Engineering
    Volume9
    Issue number1
    DOIs
    Publication statusPublished - 2012
    Publication typeA1 Journal article-refereed

    Keywords

    • cell culturing
    • electrical activity
    • human embryonic stem cells
    • microelectrode array
    • poly(dimethylsiloxane)

    Publication forum classification

    • Publication forum level 1

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