TY - JOUR
T1 - Structured PDMS chambers for enhanced human neuronal cell activity on MEA platforms
AU - Kreutzer, Joose
AU - Ylä-Outinen, Laura
AU - Kärnä, Paula
AU - Kaarela, Tiina
AU - Mikkonen, Jarno
AU - Skottman, Heli
AU - Narkilahti, Susanna
AU - Kallio, Pasi
N1 - Contribution: organisation=ase aci,FACT1=1<br/>Publisher name: Elsevier
PY - 2012
Y1 - 2012
N2 - Structured poly(dimethylsiloxane) (PDMS) chambers were designed and fabricated to enhance the signaling of human embryonic stem cell (hESC) - derived neuronal networks on microelectrode array (MEA) platforms. The structured PDMS chambers enable cell seeding on restricted areas and thus, reduce the amount of needed coating materials and cells. In addition, the neuronal cells formed spontaneously active networks faster in the structured PDMS chambers than in control chambers. In the PDMS chambers, the neuronal networks were more active and able to develop their signaling into organized signal trains faster than control cultures. The PDMS chamber design enables much more repeatable analysis and rapid growth of functional neuronal network in vitro. Moreover, due to its easy and cheap fabrication process, new configurations can be easily fabricated based on investigator requirements.
AB - Structured poly(dimethylsiloxane) (PDMS) chambers were designed and fabricated to enhance the signaling of human embryonic stem cell (hESC) - derived neuronal networks on microelectrode array (MEA) platforms. The structured PDMS chambers enable cell seeding on restricted areas and thus, reduce the amount of needed coating materials and cells. In addition, the neuronal cells formed spontaneously active networks faster in the structured PDMS chambers than in control chambers. In the PDMS chambers, the neuronal networks were more active and able to develop their signaling into organized signal trains faster than control cultures. The PDMS chamber design enables much more repeatable analysis and rapid growth of functional neuronal network in vitro. Moreover, due to its easy and cheap fabrication process, new configurations can be easily fabricated based on investigator requirements.
KW - cell culturing
KW - electrical activity
KW - human embryonic stem cells
KW - microelectrode array
KW - poly(dimethylsiloxane)
KW - cell culturing
KW - electrical activity
KW - human embryonic stem cells
KW - microelectrode array
KW - poly(dimethylsiloxane)
U2 - 10.1016/s1672-6529(11)60091-7
DO - 10.1016/s1672-6529(11)60091-7
M3 - Article
SN - 1672-6529
VL - 9
SP - 1
EP - 10
JO - Journal of Bionic Engineering
JF - Journal of Bionic Engineering
IS - 1
ER -