Superquenching of SYBRGreen dye fluorescence in complex with DNA by gold nanoparticles

E. S. Lisitsyna; O. N. Lygo; N. A. Durandin; O. V. Dement'eva; V. M. Rudoi; V. A. Kuzmin

doi:10.1134/S0018143912060057

Superquenching of SYBRGreen dye fluorescence in complex with DNA by gold nanoparticles

E. S. Lisitsyna^*
, O. N. Lygo
, N. A. Durandin
, O. V. Dement'eva
, V. M. Rudoi
, V. A. Kuzmin

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

6 Citations (Scopus)

Abstract

The influence of gold nanoparticles (diameter of about 2.5 nm) on the complex between the SYBRGreen dye and double-stranded DNA in solutions has been investigated by fluorescence spectroscopy. Strong quenching of dye fluorescence by nanosized gold particles ("superquenching"), characterized by a high Stern-Volmer constant of K_SV = 3.3 × 10⁷ L/mol, has been found. The superquenching effect in the test system is explained in terms of contribution of several processes: electron transfer, formation of aggregates of gold nanoparticles involving dye dications, and enhancement of intersystem crossing by a heavy atom (gold atoms of nanoparticles).

Original language	English
Pages (from-to)	363-367
Number of pages	5
Journal	High Energy Chemistry
Volume	46
Issue number	6
DOIs	https://doi.org/10.1134/S0018143912060057
Publication status	Published - Nov 2012
Publication type	A1 Journal article-refereed

ASJC Scopus subject areas

Physical and Theoretical Chemistry

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title = "Superquenching of SYBRGreen dye fluorescence in complex with DNA by gold nanoparticles",

abstract = "The influence of gold nanoparticles (diameter of about 2.5 nm) on the complex between the SYBRGreen dye and double-stranded DNA in solutions has been investigated by fluorescence spectroscopy. Strong quenching of dye fluorescence by nanosized gold particles ({"}superquenching{"}), characterized by a high Stern-Volmer constant of KSV = 3.3 × 107 L/mol, has been found. The superquenching effect in the test system is explained in terms of contribution of several processes: electron transfer, formation of aggregates of gold nanoparticles involving dye dications, and enhancement of intersystem crossing by a heavy atom (gold atoms of nanoparticles).",

author = "Lisitsyna, \{E. S.\} and Lygo, \{O. N.\} and Durandin, \{N. A.\} and Dement'eva, \{O. V.\} and Rudoi, \{V. M.\} and Kuzmin, \{V. A.\}",

year = "2012",

month = nov,

doi = "10.1134/S0018143912060057",

language = "English",

volume = "46",

pages = "363--367",

journal = "High Energy Chemistry",

issn = "0018-1439",

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TY - JOUR

T1 - Superquenching of SYBRGreen dye fluorescence in complex with DNA by gold nanoparticles

AU - Lisitsyna, E. S.

AU - Lygo, O. N.

AU - Durandin, N. A.

AU - Dement'eva, O. V.

AU - Rudoi, V. M.

AU - Kuzmin, V. A.

PY - 2012/11

Y1 - 2012/11

N2 - The influence of gold nanoparticles (diameter of about 2.5 nm) on the complex between the SYBRGreen dye and double-stranded DNA in solutions has been investigated by fluorescence spectroscopy. Strong quenching of dye fluorescence by nanosized gold particles ("superquenching"), characterized by a high Stern-Volmer constant of KSV = 3.3 × 107 L/mol, has been found. The superquenching effect in the test system is explained in terms of contribution of several processes: electron transfer, formation of aggregates of gold nanoparticles involving dye dications, and enhancement of intersystem crossing by a heavy atom (gold atoms of nanoparticles).

AB - The influence of gold nanoparticles (diameter of about 2.5 nm) on the complex between the SYBRGreen dye and double-stranded DNA in solutions has been investigated by fluorescence spectroscopy. Strong quenching of dye fluorescence by nanosized gold particles ("superquenching"), characterized by a high Stern-Volmer constant of KSV = 3.3 × 107 L/mol, has been found. The superquenching effect in the test system is explained in terms of contribution of several processes: electron transfer, formation of aggregates of gold nanoparticles involving dye dications, and enhancement of intersystem crossing by a heavy atom (gold atoms of nanoparticles).

UR - https://www.scopus.com/pages/publications/84870905943

U2 - 10.1134/S0018143912060057

DO - 10.1134/S0018143912060057

M3 - Article

AN - SCOPUS:84870905943

SN - 0018-1439

VL - 46

SP - 363

EP - 367

JO - High Energy Chemistry

JF - High Energy Chemistry

IS - 6

ER -

Superquenching of SYBRGreen dye fluorescence in complex with DNA by gold nanoparticles

Abstract

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