TY - JOUR
T1 - Surface modification of silicate, borosilicate, and phosphate bioactive glasses to improve/control protein adsorption
T2 - PART II
AU - Gobbo, Virginia Alessandra
AU - Turkki, Paula
AU - Santos Dias Palma, Cristina
AU - Parihar, Vijay Singh
AU - Vernè, Enrica
AU - Spriano, Silvia
AU - Sanches Ribeiro, Andre
AU - Hytönen, Vesa P.
AU - Massera, Jonathan
N1 - Funding Information:
This project was funded by the European Union's Horizon 2020 Research and Innovation Program under the Marie Skłodowska-Curie grant agreement [No 860462 (PREMUROSA)]. The authors also acknowledge the Academy of Finland, Sigrid Juselius Foundation, and Cancer Foundation of Finland for their financial support. Biocenter Finland (BF) and the Tampere Imaging Facility (TIF) are acknowledged for their infrastructure support.
Funding Information:
This project was funded by the European Union's Horizon 2020 Research and Innovation Program under the Marie Skłodowska-Curie grant agreement [No 860462 (PREMUROSA)]. The authors also acknowledge the Academy of Finland , Sigrid Juselius Foundation , and Cancer Foundation of Finland for their financial support. Biocenter Finland (BF) and the Tampere Imaging Facility (TIF) are acknowledged for their infrastructure support.
Publisher Copyright:
© 2022 The Authors
PY - 2023
Y1 - 2023
N2 - Bioactive glasses (BGs) are characterized by high biocompatibility and bioactivity and are particularly promising for bone tissue regeneration. Once implanted, the BGs interact with the environment and adsorb chemical moieties and biomolecules. Proteins in body fluids are critical for the success of implants, because the adsorption of specific proteins can either promote or inhibit the adhesion of surrounding tissue or other factors such as bacteria. Controlling protein adsorption by tailoring the surface properties of implanted biomaterials is fundamental. This can determine the fate of the implant. In the current study, four BG compositions (two silicates, one borosilicate, and one phosphate glass) and three model proteins (fibronectin, chimeric avidin, and streptavidin) were considered. Each BG was surface pretreated, and the adsorption of fluorescently labeled fibronectin, chimeric avidin, or streptavidin was monitored. Untreated surfaces were used as controls. The amount and spatial distribution of each protein were estimated by confocal microscopy in fluorescence modality, followed by protein clustering analysis. Although streptavidin was not adsorbed efficiently on any of the considered substrates, BGs were successfully coated with fibronectin and chimeric avidin. Both proteins showed different affinities and surface distributions as functions of the implemented pretreatment on each substrate.
AB - Bioactive glasses (BGs) are characterized by high biocompatibility and bioactivity and are particularly promising for bone tissue regeneration. Once implanted, the BGs interact with the environment and adsorb chemical moieties and biomolecules. Proteins in body fluids are critical for the success of implants, because the adsorption of specific proteins can either promote or inhibit the adhesion of surrounding tissue or other factors such as bacteria. Controlling protein adsorption by tailoring the surface properties of implanted biomaterials is fundamental. This can determine the fate of the implant. In the current study, four BG compositions (two silicates, one borosilicate, and one phosphate glass) and three model proteins (fibronectin, chimeric avidin, and streptavidin) were considered. Each BG was surface pretreated, and the adsorption of fluorescently labeled fibronectin, chimeric avidin, or streptavidin was monitored. Untreated surfaces were used as controls. The amount and spatial distribution of each protein were estimated by confocal microscopy in fluorescence modality, followed by protein clustering analysis. Although streptavidin was not adsorbed efficiently on any of the considered substrates, BGs were successfully coated with fibronectin and chimeric avidin. Both proteins showed different affinities and surface distributions as functions of the implemented pretreatment on each substrate.
U2 - 10.1016/j.ceramint.2022.12.157
DO - 10.1016/j.ceramint.2022.12.157
M3 - Article
AN - SCOPUS:85144794611
SN - 0272-8842
VL - 49
SP - 12856
EP - 12865
JO - Ceramics International
JF - Ceramics International
IS - 8
ER -