Switchavidin: Reversible biotin-avidin-biotin bridges with high affinity and specificity

Barbara Taskinen; Dominik Zauner; Soili I. Lehtonen; Masi Koskinen; Chloe Thomson; Niklas Kähkönen; Sampo Kukkurainen; Juha A E Määttä; Teemu O. Ihalainen; Markku S. Kulomaa; Hermann J. Gruber; Vesa P. Hytönen

doi:10.1021/bc500462w

Switchavidin: Reversible biotin-avidin-biotin bridges with high affinity and specificity

Barbara Taskinen
, Dominik Zauner
, Soili I. Lehtonen
, Masi Koskinen
, Chloe Thomson
, Niklas Kähkönen
, Sampo Kukkurainen
, Juha A E Määttä
, Teemu O. Ihalainen
, Markku S. Kulomaa
, Hermann J. Gruber
, Vesa P. Hytönen

Department of Clinical Chemistry

Research output: Contribution to journal › Article › Scientific › peer-review

34 Citations (Scopus)

Abstract

Switchavidin is a chicken avidin mutant displaying reversible binding to biotin, an improved binding affinity toward conjugated biotin, and low nonspecific binding due to reduced surface charge. These properties make switchavidin an optimal tool in biosensor applications for the reversible immobilization of biotinylated proteins on biotinylated sensor surfaces. Furthermore, switchavidin opens novel possibilities for patterning, purification, and labeling. (Graph Presented).

Original language	English
Pages (from-to)	2233-2243
Number of pages	11
Journal	Bioconjugate Chemistry
Volume	25
Issue number	12
DOIs	https://doi.org/10.1021/bc500462w
Publication status	Published - 2014
Publication type	A1 Journal article-refereed

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10.1021/bc500462w

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@article{1b558b06df86421490f4d89645daa5cc,

title = "Switchavidin: Reversible biotin-avidin-biotin bridges with high affinity and specificity",

abstract = "Switchavidin is a chicken avidin mutant displaying reversible binding to biotin, an improved binding affinity toward conjugated biotin, and low nonspecific binding due to reduced surface charge. These properties make switchavidin an optimal tool in biosensor applications for the reversible immobilization of biotinylated proteins on biotinylated sensor surfaces. Furthermore, switchavidin opens novel possibilities for patterning, purification, and labeling. (Graph Presented).",

author = "Barbara Taskinen and Dominik Zauner and Lehtonen, \{Soili I.\} and Masi Koskinen and Chloe Thomson and Niklas K{\"a}hk{\"o}nen and Sampo Kukkurainen and M{\"a}{\"a}tt{\"a}, \{Juha A E\} and Ihalainen, \{Teemu O.\} and Kulomaa, \{Markku S.\} and Gruber, \{Hermann J.\} and Hyt{\"o}nen, \{Vesa P.\}",

year = "2014",

doi = "10.1021/bc500462w",

language = "English",

volume = "25",

pages = "2233--2243",

journal = "Bioconjugate Chemistry",

issn = "1043-1802",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Switchavidin: Reversible biotin-avidin-biotin bridges with high affinity and specificity

AU - Taskinen, Barbara

AU - Zauner, Dominik

AU - Lehtonen, Soili I.

AU - Koskinen, Masi

AU - Thomson, Chloe

AU - Kähkönen, Niklas

AU - Kukkurainen, Sampo

AU - Määttä, Juha A E

AU - Ihalainen, Teemu O.

AU - Kulomaa, Markku S.

AU - Gruber, Hermann J.

AU - Hytönen, Vesa P.

PY - 2014

Y1 - 2014

N2 - Switchavidin is a chicken avidin mutant displaying reversible binding to biotin, an improved binding affinity toward conjugated biotin, and low nonspecific binding due to reduced surface charge. These properties make switchavidin an optimal tool in biosensor applications for the reversible immobilization of biotinylated proteins on biotinylated sensor surfaces. Furthermore, switchavidin opens novel possibilities for patterning, purification, and labeling. (Graph Presented).

AB - Switchavidin is a chicken avidin mutant displaying reversible binding to biotin, an improved binding affinity toward conjugated biotin, and low nonspecific binding due to reduced surface charge. These properties make switchavidin an optimal tool in biosensor applications for the reversible immobilization of biotinylated proteins on biotinylated sensor surfaces. Furthermore, switchavidin opens novel possibilities for patterning, purification, and labeling. (Graph Presented).

U2 - 10.1021/bc500462w

DO - 10.1021/bc500462w

M3 - Article

AN - SCOPUS:84918539954

SN - 1043-1802

VL - 25

SP - 2233

EP - 2243

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 12

ER -

Switchavidin: Reversible biotin-avidin-biotin bridges with high affinity and specificity

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