Synchrony between Genetic Repressilators in Sister Cells in Different Temperatures

Jerome G. Chandraseelan, Samuel M.D. Oliveira, Antti Häkkinen, Sofia Startceva, Andre S. Ribeiro

    Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

    Abstract

    We used live E. coli containing synthetic genetic oscillators to study how the degree of synchrony between the genetic circuits of sister cells changes with temperature. We found that both the mean and the variability of the degree of synchrony between the fluorescence signals from sister cells are affected by temperature. Also, while most pairs of sister cells were found to be highly synchronous in each condition, the number of asynchronous pairs increased with increasing temperature, which was found to be due to disruptions in the oscillations. Finally we provide evidence that these disruptions tend to affect multiple generations as opposed to individual cells. These findings provide insight in how to design more robust synthetic circuits and in how cell division can affect their dynamics.
    Original languageEnglish
    Title of host publicationICCMSB 2015: International Conference on Computational and Molecular Systems Biology
    Pages725-728
    Number of pages4
    Publication statusPublished - 2015
    Publication typeA4 Article in conference proceedings
    EventInternational Conference on Computational and Molecular Systems Biology - , United Kingdom
    Duration: 1 Jan 2015 → …

    Publication series

    NameProceedings of the World Academy of Science, Engineering and Technology
    ISSN (Print)2010-376X
    ISSN (Electronic)2010-3778

    Conference

    ConferenceInternational Conference on Computational and Molecular Systems Biology
    Country/TerritoryUnited Kingdom
    Period1/01/15 → …

    Keywords

    • repressilator, robustness, synchrony, synthetic biology

    Publication forum classification

    • No publication forum level

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