The precision of the symmetry in Z-ring placement in Escherichia coli is hampered at critical temperatures

Ramakanth Neeli-Venkata, Samuel M.D. Oliveira, Leonardo Martins, Sofia Startceva, Mohamed Bahrudeen, Jose M. Fonseca, Marco Minoia, Andre S. Ribeiro

    Research output: Contribution to journalArticleScientificpeer-review

    1 Citation (Scopus)
    3 Downloads (Pure)

    Abstract

    Cell division in Escherichia coli is morphologically symmetric due to, among other things, the ability of these cells to place the Z-ring at midcell. Studies have reported that, at sub-optimal temperatures, this symmetry decreases at the single-cell level, but the causes remain unclear. Using fluorescence microscopy, we observe FtsZ-GFP and DAPI-stained nucleoids to assess the robustness of the symmetry of Z-ring formation and positioning in individual cells under sub-optimal and critical temperatures. We find the Z-ring formation and positioning to be robust at sub-optimal temperatures, as the Z-ring's mean width, density and displacement from midcell maintain similar levels of correlation to one another as at optimal temperatures. However, at critical temperatures, the Z-ring displacement from midcell is greatly increased. We present evidence showing that this is due to enhanced distance between the replicated nucleoids and, thus, reduced Z-ring density, which explains the weaker precision in setting a morphologically symmetric division site. This also occurs in rich media and is cumulative, i.e. combining richer media and critically high temperatures enhances the asymmetries in division, which is evidence that the causes are biophysical. To further support this, we show that the effects are reversible, i.e. shifting cells from optimal to critical, and then to optimal again, reduces and then enhances the symmetry in Z-ring positioning, respectively, as the width and density of the Z-ring return to normal values. Overall, our findings show that the Z-ring positioning in E. coli is a robust biophysical process under sub-optimal temperatures, and that critical temperatures cause significant asymmetries in division.

    Original languageEnglish
    Article number056002
    JournalPhysical Biology
    Volume15
    Issue number5
    DOIs
    Publication statusPublished - 18 May 2018
    Publication typeA1 Journal article-refereed

    Keywords

    • asymmetry in cell division
    • critical temperatures
    • Escherichia coli
    • single-cell microscopy
    • Z-ring formation and positioning

    Publication forum classification

    • Publication forum level 1

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Molecular Biology
    • Cell Biology

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