Thermophilic versus mesophilic dark fermentation in xylose-fed fluidised bed reactors: Biohydrogen production and active microbial community

Paolo Dessì, Estefania Porca, Nicholas R. Waters, Aino-Maija Lakaniemi, Gavin Collins, Piet N.L. Lens

    Research output: Contribution to journalArticleScientificpeer-review

    14 Citations (Scopus)
    65 Downloads (Pure)

    Abstract

    Dark fermentative biohydrogen production in a thermophilic, xylose-fed (50 mM) fluidised bed reactor (FBR) was evaluated in the temperature range 55-70 °C with 5-degree increments and compared with a mesophilic FBR operated constantly at 37 °C. A significantly higher (p = 0.05) H2 yield was obtained in the thermophilic FBR, which stabilised at about 1.2 mol H2 mol-1 xylose (36% of the theoretical maximum) at 55 and 70 °C, and at 0.8 mol H2 mol-1 xylose at 60 and 65 °C, compared to the mesophilic FBR (0.5 mol H2 mol-1 xylose). High-throughput sequencing of the reverse-transcribed 16S rRNA, done for the first time on biohydrogen producing reactors, indicated that Thermoanaerobacterium was the prevalent active microorganism in the thermophilic FBR, regardless of the operating temperature. The active microbial community in the mesophilic FBR was mainly composed of Clostridium and Ruminiclostridium at 37 °C. Thermophilic dark fermentation was shown to be suitable for treatment of high temperature, xylose-containing wastewaters, as it resulted in a higher energy output compared to the mesophilic counterpart.

    Original languageEnglish
    Pages (from-to)5473-5485
    JournalInternational Journal of Hydrogen Energy
    Volume43
    Issue number11
    Early online date2018
    DOIs
    Publication statusPublished - 2018
    Publication typeA1 Journal article-refereed

    Keywords

    • Active community
    • Biohydrogen
    • FBR
    • MiSeq
    • Thermoanaerobacterium
    • Thermophilic

    Publication forum classification

    • Publication forum level 1

    ASJC Scopus subject areas

    • Renewable Energy, Sustainability and the Environment
    • Fuel Technology
    • Condensed Matter Physics
    • Energy Engineering and Power Technology

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