Ultrasensitive and rapid diagnostic tool for detection of Acanthamoeba castellanii

Susanna Haapanen; Maarit S. Patrikainen; Seppo Parkkila

doi:10.1016/j.diagmicrobio.2023.116014

Ultrasensitive and rapid diagnostic tool for detection of Acanthamoeba castellanii

Susanna Haapanen^*
, Maarit S. Patrikainen
, Seppo Parkkila

^*Corresponding author for this work

Department of Clinical Microbiology

Research output: Contribution to journal › Article › Scientific › peer-review

3 Citations (Scopus)

43 Downloads (Pure)

Abstract

Acanthamoeba keratitis is a devastating infectious disease of the cornea caused by an opportunistic amoeba, Acanthamoeba castellanii. It is poorly recognized, and diagnostic delays can lead to irreversible damage to the vision. The gold standard for diagnosis has been a sample culture that lasts approximately 2 weeks. Nevertheless, the essence of time has led to the need for an accurate and fast technique to detect A. castellanii from a sample. We developed both traditional and quantitative real-time-PCR-based methods to detect A. castellanii in less than 3 hours and with the sensitivity of one amoeba. Diagnostic laboratories can select the best-suited method for their purposes from 2 comparable methods. The correct treatment can be initiated from the emergency room when the diagnosis has been made quickly within a few hours, hence saving the patient from long-term complications.

Original language	English
Article number	116014
Journal	Diagnostic Microbiology and Infectious Disease
Volume	107
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2023.116014
Publication status	Published - 2023
Publication type	A1 Journal article-refereed

Funding

The authors acknowledge the Biocenter Finland (BF) and Tampere Genomics Facility for the sequencing service. We also thank Marianne Kuuslahti for her expertise in setting up and maintaining amoeba culture. Figure 1 was created with BioRender.com. The work was supported by grants from the Finnish Medical Foundation (SH, grant number 5299) and the Academy of Finland (SP, grant number 348972). SP, MSP and SH designed the experiments; SH conducted the PCR laboratory work; SH and MSP conducted the qRT‒PCR laboratory work; SH prepared the first manuscript draft; all authors wrote and finalized the article. The work was supported by grants from the Finnish Medical Foundation (SH, grant number 5299 ) and the Academy of Finland (SP, grant number 348972 ).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Acanthamoeba keratitis
Diagnostic method
Polymerase chain reaction

Publication forum classification

Publication forum level 1

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

Access to Document

10.1016/j.diagmicrobio.2023.116014Licence: CC BY

1-s2.0-S0732889323001244-mainFinal published version, 651 KBLicence: CC BY

https://urn.fi/URN:NBN:fi:tuni-202308187667Licence: CC BY

Cite this

@article{e42692bf24904a0d82f7d6e92b7d5dae,

title = "Ultrasensitive and rapid diagnostic tool for detection of Acanthamoeba castellanii",

abstract = "Acanthamoeba keratitis is a devastating infectious disease of the cornea caused by an opportunistic amoeba, Acanthamoeba castellanii. It is poorly recognized, and diagnostic delays can lead to irreversible damage to the vision. The gold standard for diagnosis has been a sample culture that lasts approximately 2 weeks. Nevertheless, the essence of time has led to the need for an accurate and fast technique to detect A. castellanii from a sample. We developed both traditional and quantitative real-time-PCR-based methods to detect A. castellanii in less than 3 hours and with the sensitivity of one amoeba. Diagnostic laboratories can select the best-suited method for their purposes from 2 comparable methods. The correct treatment can be initiated from the emergency room when the diagnosis has been made quickly within a few hours, hence saving the patient from long-term complications.",

keywords = "Acanthamoeba keratitis, Diagnostic method, Polymerase chain reaction",

author = "Susanna Haapanen and Patrikainen, \{Maarit S.\} and Seppo Parkkila",

note = "Funding Information: The authors acknowledge the Biocenter Finland (BF) and Tampere Genomics Facility for the sequencing service. We also thank Marianne Kuuslahti for her expertise in setting up and maintaining amoeba culture. Figure 1 was created with BioRender.com. The work was supported by grants from the Finnish Medical Foundation (SH, grant number 5299) and the Academy of Finland (SP, grant number 348972). SP, MSP and SH designed the experiments; SH conducted the PCR laboratory work; SH and MSP conducted the qRT‒PCR laboratory work; SH prepared the first manuscript draft; all authors wrote and finalized the article. Funding Information: The work was supported by grants from the Finnish Medical Foundation (SH, grant number 5299 ) and the Academy of Finland (SP, grant number 348972 ). Publisher Copyright: {\textcopyright} 2023 The Author(s)",

year = "2023",

doi = "10.1016/j.diagmicrobio.2023.116014",

language = "English",

volume = "107",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Ultrasensitive and rapid diagnostic tool for detection of Acanthamoeba castellanii

AU - Haapanen, Susanna

AU - Patrikainen, Maarit S.

AU - Parkkila, Seppo

N1 - Funding Information: The authors acknowledge the Biocenter Finland (BF) and Tampere Genomics Facility for the sequencing service. We also thank Marianne Kuuslahti for her expertise in setting up and maintaining amoeba culture. Figure 1 was created with BioRender.com. The work was supported by grants from the Finnish Medical Foundation (SH, grant number 5299) and the Academy of Finland (SP, grant number 348972). SP, MSP and SH designed the experiments; SH conducted the PCR laboratory work; SH and MSP conducted the qRT‒PCR laboratory work; SH prepared the first manuscript draft; all authors wrote and finalized the article. Funding Information: The work was supported by grants from the Finnish Medical Foundation (SH, grant number 5299 ) and the Academy of Finland (SP, grant number 348972 ). Publisher Copyright: © 2023 The Author(s)

PY - 2023

Y1 - 2023

N2 - Acanthamoeba keratitis is a devastating infectious disease of the cornea caused by an opportunistic amoeba, Acanthamoeba castellanii. It is poorly recognized, and diagnostic delays can lead to irreversible damage to the vision. The gold standard for diagnosis has been a sample culture that lasts approximately 2 weeks. Nevertheless, the essence of time has led to the need for an accurate and fast technique to detect A. castellanii from a sample. We developed both traditional and quantitative real-time-PCR-based methods to detect A. castellanii in less than 3 hours and with the sensitivity of one amoeba. Diagnostic laboratories can select the best-suited method for their purposes from 2 comparable methods. The correct treatment can be initiated from the emergency room when the diagnosis has been made quickly within a few hours, hence saving the patient from long-term complications.

AB - Acanthamoeba keratitis is a devastating infectious disease of the cornea caused by an opportunistic amoeba, Acanthamoeba castellanii. It is poorly recognized, and diagnostic delays can lead to irreversible damage to the vision. The gold standard for diagnosis has been a sample culture that lasts approximately 2 weeks. Nevertheless, the essence of time has led to the need for an accurate and fast technique to detect A. castellanii from a sample. We developed both traditional and quantitative real-time-PCR-based methods to detect A. castellanii in less than 3 hours and with the sensitivity of one amoeba. Diagnostic laboratories can select the best-suited method for their purposes from 2 comparable methods. The correct treatment can be initiated from the emergency room when the diagnosis has been made quickly within a few hours, hence saving the patient from long-term complications.

KW - Acanthamoeba keratitis

KW - Diagnostic method

KW - Polymerase chain reaction

U2 - 10.1016/j.diagmicrobio.2023.116014

DO - 10.1016/j.diagmicrobio.2023.116014

M3 - Article

C2 - 37506594

AN - SCOPUS:85166015469

SN - 0732-8893

VL - 107

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 2

M1 - 116014

ER -

Ultrasensitive and rapid diagnostic tool for detection of Acanthamoeba castellanii

Abstract

Funding

UN SDGs

Keywords

Publication forum classification

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this