TY - JOUR
T1 - Unveiling extracellular matrix assembly
T2 - Insights and approaches through bioorthogonal chemistry
AU - Tavakoli, Shima
AU - Evans, Austin
AU - Oommen, Oommen P.
AU - Creemers, Laura
AU - Nandi, Jharna Barman
AU - Hilborn, Jöns
AU - Varghese, Oommen P.
N1 - Funding Information:
Financial assistance for this review was provided by the Swedish Strategic Research ‘StemTherapy’ (Dnr 2009–1035 ), the Swedish Research Council (Dnr 2020–04025 ), European Union's EUREKA/Eurostars-2 (nr. 2020–03601 ), and European Union's Horizon 2020 Marie Sklodowska-Curie H2020-MSCA-ITN-ETN-2020 (nr. 955335 ) and the Dutch Arthritis Foundation ( LLP12 ).
Funding Information:
Financial assistance for this review was provided by the Swedish Strategic Research ‘StemTherapy’ (Dnr 2009–1035), the Swedish Research Council (Dnr 2020–04025), European Union's EUREKA/Eurostars-2 (nr. 2020–03601), and European Union's Horizon 2020 Marie Sklodowska-Curie H2020-MSCA-ITN-ETN-2020 (nr. 955335) and the Dutch Arthritis Foundation (LLP12).
Publisher Copyright:
© 2023
PY - 2023/10
Y1 - 2023/10
N2 - Visualizing cells, tissues, and their components specifically without interference with cellular functions, such as biochemical reactions, and cellular viability remains important for biomedical researchers worldwide. For an improved understanding of disease progression, tissue formation during development, and tissue regeneration, labeling extracellular matrix (ECM) components secreted by cells persists is required. Bioorthogonal chemistry approaches offer solutions to visualizing and labeling ECM constituents without interfering with other chemical or biological events. Although biorthogonal chemistry has been studied extensively for several applications, this review summarizes the recent advancements in using biorthogonal chemistry specifically for metabolic labeling and visualization of ECM proteins and glycosaminoglycans that are secreted by cells and living tissues. Challenges, limitations, and future directions surrounding biorthogonal chemistry involved in the labeling of ECM components are discussed. Finally, potential solutions for improvements to biorthogonal chemical approaches are suggested. This would provide theoretical guidance for labeling and visualization of de novo proteins and polysaccharides present in ECM that are cell-secreted for example during tissue remodeling or in vitro differentiation of stem cells.
AB - Visualizing cells, tissues, and their components specifically without interference with cellular functions, such as biochemical reactions, and cellular viability remains important for biomedical researchers worldwide. For an improved understanding of disease progression, tissue formation during development, and tissue regeneration, labeling extracellular matrix (ECM) components secreted by cells persists is required. Bioorthogonal chemistry approaches offer solutions to visualizing and labeling ECM constituents without interfering with other chemical or biological events. Although biorthogonal chemistry has been studied extensively for several applications, this review summarizes the recent advancements in using biorthogonal chemistry specifically for metabolic labeling and visualization of ECM proteins and glycosaminoglycans that are secreted by cells and living tissues. Challenges, limitations, and future directions surrounding biorthogonal chemistry involved in the labeling of ECM components are discussed. Finally, potential solutions for improvements to biorthogonal chemical approaches are suggested. This would provide theoretical guidance for labeling and visualization of de novo proteins and polysaccharides present in ECM that are cell-secreted for example during tissue remodeling or in vitro differentiation of stem cells.
KW - Bioorthogonal chemistry
KW - Extracellular matrix
KW - Metabolic labeling
KW - Visualizing cells
U2 - 10.1016/j.mtbio.2023.100768
DO - 10.1016/j.mtbio.2023.100768
M3 - Review Article
AN - SCOPUS:85167437644
SN - 2590-0064
VL - 22
JO - Materials Today Bio
JF - Materials Today Bio
M1 - 100768
ER -