Use of factorial experimental design to study the effects of iron and sulfur on growth of Scenedesmus acuminatus with different nitrogen sources

Ran Tao, Robert Bair, Aino Maija Lakaniemi, Eric D. van Hullebusch, Jukka A. Rintala

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)
13 Downloads (Pure)

Abstract

The aim of this study was to determine the combined effects of iron and sulfur on microalgal biomass concentration and removal efficiency of nitrogenous compounds using factorial design. Scenedesmus acuminatus (currently accepted name Tetradesmus lagerheimii) was separately cultivated in batch photobioreactors using modified N-8 media with two nitrogen sources, nitrate, and ammonium. To study the interaction effect between iron and sulfur and to reduce the total number of experimentally studied combinations, a factorial design was used. Three iron (0.1, 1, and 1.9 mg L−1) and three sulfur concentrations (3.7, 20, and 35.8 mg L−1) were employed to the modified N-8 media in this study. The results show that the final microalgal biomass concentration and nitrogen removal efficiency were more sensitive to the changes in iron and sulfur concentrations in the media with nitrate than with ammonium possibly because of the different assimilation mechanisms used by microalgae for these two nitrogen sources. The created models demonstrated that iron had a statistically significant effect on the microalgal biomass concentration and nitrate removal efficiency while sulfur did not. In addition, the interaction effect between iron and sulfur was not significant on microalgal biomass concentration and nitrogen removal. In synthetic medium with nitrate as nitrogen source, the highest microalgal biomass concentration was obtained with 1.0 mg L−1 iron and 35.8 mg L−1 sulfur.

Original languageEnglish
Pages (from-to)221–231
Number of pages11
JournalJournal of Applied Phycology
Volume32
Issue number1
Early online date2019
DOIs
Publication statusPublished - 2020
Publication typeA1 Journal article-refereed

Keywords

  • Chlorophyceae
  • Factorial experimental design
  • Iron
  • Microalgal growth
  • Nitrogen removal
  • Sulfur

Publication forum classification

  • Publication forum level 1

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Fingerprint

Dive into the research topics of 'Use of factorial experimental design to study the effects of iron and sulfur on growth of Scenedesmus acuminatus with different nitrogen sources'. Together they form a unique fingerprint.

Cite this