X-ray microtomography of collagen and polylactide samples in liquids

    Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

    1 Citation (Scopus)

    Abstract

    Methods to image and assess the microstructure of polymer based biomaterials in liquid phase, for example cell culture medium, are well warranted. X-ray microtomography could provide a mean to visualize and analyze such structures. However, the density of such polymers is close to that of water and hence the inherent X-ray contrast is poor. The material can provide good contrast when dry, however, if the materials contain cells and are dried, the cell morphology may be distorted. Moreover the entire structure of these water containing materials are deformed in the drying process. In this paper we tested phosphotungstic acid (PTA) staining to improve the contrast. We imaged collagen and PLA samples, as well as collagen-PLA composites with μCT in air, water and alcohol. The methods were compared visually and with contrast to noise ratio calculated from the images. Our results demonstrate that with alcohol the PLA can be imaged also in liquid phase. PTA staining seems to be a good method to increase the contrast for collagen in μCT imaging.

    Original languageEnglish
    Title of host publicationXIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016
    Subtitle of host publicationMEDICON 2016, March 31st–April 2nd 2016, Paphos, Cyprus
    PublisherSpringer Verlag
    Pages420-424
    Number of pages5
    ISBN (Print)9783319327013
    DOIs
    Publication statusPublished - 2016
    Publication typeA4 Article in a conference publication
    EventMediterranean Conference on Medical and Biological Engineering and Computing -
    Duration: 1 Jan 2000 → …

    Publication series

    NameIFMBE Proceedings
    Volume57
    ISSN (Print)1680-0737

    Conference

    ConferenceMediterranean Conference on Medical and Biological Engineering and Computing
    Period1/01/00 → …

    Keywords

    • Collagen
    • Polylactide
    • Staining
    • μCT imaging

    Publication forum classification

    • Publication forum level 1

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Bioengineering

    Fingerprint Dive into the research topics of 'X-ray microtomography of collagen and polylactide samples in liquids'. Together they form a unique fingerprint.

    Cite this