Degradation and Stability Observations of Bioactivated Gellan Gum Hydrogels in vitro and in vivo

Description

[Poster presentation]

Introdcution
Hydrogels have gained a considerable reputation as soft tissue mimics. While mechanical, viscoelastic, cytocompatibility and diffusion properties are often mentioned in this regard, also degradation is crucial for tissue engineering purposes1. Degradation is required for the removal of regenerative devices from the body, as well as for the cell-driven remodeling in tissue models. The mechanism of degradation is governed primarily by the chemical structure of the polymer and crosslinking type. Degradability of hydrogels is tested via mass loss or swelling observations in vitro, often with cell-free analogues and a variety of other simplifications to model the target environment. It remains challenging to correlate these cell-free in vitro observations to cell-mediated degradation to physiological resorption in vivo.
Gellan gum (GG) is a bacterial polysaccharide with excellent gelation and mechanical properties, however it lacks bioactivity2. We have investigated four GG-based hydrogels in our recent work3, including ionically crosslinked purified GG (NaGG) and avidin-modified NaGG (NaGG-avd), showcasing the modular incorporation of fibronectin via avidin-biotin binding4. The other two hydrogel formulations are formed by covalent crosslinking of oxidized GG and carbohydrazide (gelaCDH) or adipic hydrazide (gelaADH) modified gelatin, utilizing gelatin’s native bioactivity2. We investigated the viscoelastic properties, the cell response of a endothelial – adipose stem cell co-culture, as well as the tissue response to subcutaneous implants of these hydrogels in vivo3. Here, we will focus on the degradation observations made throughout the different in vitro and in vivo environments.

Aikajakso	4 syysk. 2022 → 8 syysk. 2022
Tapahtuman otsikko	32nd Annual Conference of the European Society for Biomaterials
Tapahtuman tyyppi	Conference
Sijainti	Bordeaux, RanskaNäytä kartalla
Tunnustuksen arvo	International