We provide a dataset of microelectrode array recordings (MEA) from human pluripotent stem cell (hPSC)-derived and rat embryonic cortical neurons during in vitro maturation and pharmacological treatment at their mature stage. Together with the recorded raw MEA data, we share the analysis code that produces the key scientific findings published previously with this dataset (Hyvärinen et al., 2019), including PCA analysis to reveal the distinction between rat and human cell activity features. The data enables evaluation and comparison of hPSC-derived and rat cortical cultures in terms of functional development and response to pharmacological treatment. The 12500 Hz sampled data is recorded with Axion 12-well plates (for developmental tracking) and 48-well plates (for pharmacological experiments). Raw data constitute time series of extracellularly recorded voltage values in Volts. The duration of the recordings varies from 10 to 30 minutes depending on the experimental set-up. Each raw MEA data file contains organised meta-data. This dataset is valuable for experimental and computational neuroscientists and any signal analyst with interest for neuronal signalling. The accurate detailed description of data is delivered in the corresponding publication (Kapucu et al., submitted).
|7 maalisk. 2021
- 1182 Biokemia, solu- ja molekyylibiologia