FWHM microscopy data

To measure the optical aberrations caused by different silicone films, we used the full width at half maximum (FWHM) method. 0.2 µm Ø fluorescent FluoSpheres™ Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA were adsorebd to the surface of different silicone membranes by physisorption. These point like objects were imaged with Zeiss LSM 780 confocal microscope with C-Apochromat 40x/1.20 W Korr M27 objective. Z-stacks were taken.1024x1024 pixels, pixel size 103.8 nmZ-step 200nmPixel dwell time 3.9393939393939396e-007 sChannel 1 Argon 488 nmChannel 2 DPSS 561 nm