A large-scale analysis of genetic variants within putative miRNA binding sites in prostate cancer

Shane Stegeman; Ernest Amankwah; Kerenaftali Klein; Trace A O'Mara; Donghwa Kim; Hui Yi Lin; Jennifer Permuth-Wey; Thomas A. Sellers; Srilakshmi Srinivasan; Rosalind Eeles; Doug Easton; Zsofia Kote-Jarai; Ali Amin Al Olama; Sara Benlloch; Kenneth Muir; Graham G. Giles; Fik Wiklund; Henrik Gronberg; Christopher A. Haiman; Johanna Schleutker; Børge G. Nordestgaard; Ruth C. Travis; David Neal; Paul Pharoah; Kay Tee Khaw; Janet L. Stanford; William J. Blot; Stephen Thibodeau; Christiane Maier; Adam S. Kibel; Cezary Cybulski; Lisa Cannon-Albright; Hermann Brenner; Radka Kaneva; Manuel R. Teixeira; Amanda B. Spurdle; Judith A. Clements; Jong Y. Park; Jyotsna Batra; Margaret Cook; Angela Morgan; Artitaya Lophatananon; Cyril Fisher; Daniel Leongamornlert; Edward J. Saunders; Emma J. Sawyer; Koveela Govindasami; Malgorzata Tymrakiewicz; Michelle Guy; Naomi Livni; Rosemary Wilkinson; Sara Jugurnauth-Little; Steve Hazel; Tokhir Dadaev; John Pedersen; John L. Hopper; Melissa C. Southey; Ami Karlsson; Carin Cavalli-Bjoerkman; Jan Erik Johansson; Jan Adolfson; Markus Aly; Michael Broms; Paer Stattin; Brian E. Henderson; Fredrick Schumacher; Anssi Auvinen; Kimmo Taari; Liisa Maeaettaenen; Paula Kujala; Teemu Murtola; Teuvo L.J. Tammela; Tiina Wahlfors; Andreas Roder; Peter Iversen; Peter Klarskov; Sune F. Nielsen; Maren Weischer; Tim J. Key; Hans Wallinder; Sven Gustafsson; Jenny L. Donovan; Freddie Hamdy; Anne George; Athene Lane; Gemma Marsden; Michael Davis; Paul Brown; Nora Pashayan; Sarah Holt; Lisa B. Signorello; Wei Zheng; Liang Wang; Lori Tillmans; Shaun Riska; Antje Rinckleb; Kathleen Herkommer; Manuel Luedeke; Walther Vogel; Dominika Wokozorczyk; Jan Lubiski; Wojciech Kluzniak; Aida K. Dieffenbach; Christa Stegmaier; Volker Arndt; Babu Zachariah; Hyun Park; Julio Pow-Sang; Maria Rincon; Selina Radlein; Aleksandrina Vlahova; Atanaska Mitkova; Chavdar Slavov; Darina Kachakova; Elenko Popov; Svetlana Christova; Tihomir Dikov; Vanio Mitev; Felicity Lose; Joana Santos; Joao Barros-Silva; Paula Paulo; Pedro Pinto; Rui Henrique; Sofia Maia; Gail Risbridger; Renea Taylor; Wayne Tilley; Lisa Butler; Lisa Horvath; Trina Yeadon; Allison Eckert; Glen Wood; Peter Heathcote; Greg Malone; Kris Kerr; Megan Turner; Angus Collins

doi:10.1158/2159-8290.cd-14-1057

A large-scale analysis of genetic variants within putative miRNA binding sites in prostate cancer

Shane Stegeman, Ernest Amankwah, Kerenaftali Klein, Trace A O'Mara, Donghwa Kim, Hui Yi Lin, Jennifer Permuth-Wey, Thomas A. Sellers, Srilakshmi Srinivasan, Rosalind Eeles, Doug Easton, Zsofia Kote-Jarai, Ali Amin Al Olama, Sara Benlloch, Kenneth Muir, Graham G. Giles, Fik Wiklund, Henrik Gronberg, Christopher A. Haiman, Johanna SchleutkerBørge G. Nordestgaard, Ruth C. Travis, David Neal, Paul Pharoah, Kay Tee Khaw, Janet L. Stanford, William J. Blot, Stephen Thibodeau, Christiane Maier, Adam S. Kibel, Cezary Cybulski, Lisa Cannon-Albright, Hermann Brenner, Radka Kaneva, Manuel R. Teixeira, Amanda B. Spurdle, Judith A. Clements, Jong Y. Park, Jyotsna Batra, Margaret Cook, Angela Morgan, Artitaya Lophatananon, Cyril Fisher, Daniel Leongamornlert, Edward J. Saunders, Emma J. Sawyer, Koveela Govindasami, Malgorzata Tymrakiewicz, Michelle Guy, Naomi Livni, Rosemary Wilkinson, Sara Jugurnauth-Little, Steve Hazel, Tokhir Dadaev, John Pedersen, John L. Hopper, Melissa C. Southey, Ami Karlsson, Carin Cavalli-Bjoerkman, Jan Erik Johansson, Jan Adolfson, Markus Aly, Michael Broms, Paer Stattin, Brian E. Henderson, Fredrick Schumacher, Anssi Auvinen, Kimmo Taari, Liisa Maeaettaenen, Paula Kujala, Teemu Murtola, Teuvo L.J. Tammela, Tiina Wahlfors, Andreas Roder, Peter Iversen, Peter Klarskov, Sune F. Nielsen, Maren Weischer, Tim J. Key, Hans Wallinder, Sven Gustafsson, Jenny L. Donovan, Freddie Hamdy, Anne George, Athene Lane, Gemma Marsden, Michael Davis, Paul Brown, Nora Pashayan, Sarah Holt, Lisa B. Signorello, Wei Zheng, Liang Wang, Lori Tillmans, Shaun Riska, Antje Rinckleb, Kathleen Herkommer, Manuel Luedeke, Walther Vogel, Dominika Wokozorczyk, Jan Lubiski, Wojciech Kluzniak, Aida K. Dieffenbach, Christa Stegmaier, Volker Arndt, Babu Zachariah, Hyun Park, Julio Pow-Sang, Maria Rincon, Selina Radlein, Aleksandrina Vlahova, Atanaska Mitkova, Chavdar Slavov, Darina Kachakova, Elenko Popov, Svetlana Christova, Tihomir Dikov, Vanio Mitev, Felicity Lose, Joana Santos, Joao Barros-Silva, Paula Paulo, Pedro Pinto, Rui Henrique, Sofia Maia, Gail Risbridger, Renea Taylor, Wayne Tilley, Lisa Butler, Lisa Horvath, Trina Yeadon, Allison Eckert, Glen Wood, Peter Heathcote, Greg Malone, Kris Kerr, Megan Turner, Angus Collins

Kliinisen kemian yksikkö

Tutkimustuotos: Artikkeli › Tieteellinen › vertaisarvioitu

54 Sitaatiot (Scopus)

Abstrakti

<p>Prostate cancer is the second most common malignancy among men worldwide. Genome-wide association studies have identified 100 risk variants for prostate cancer, which can explain approximately 33% of the familial risk of the disease. We hypothesized that a comprehensive analysis of genetic variations found within the 3′ untranslated region of genes predicted to affect miRNA binding (miRSNP) can identify additional prostate cancer risk variants. We investigated the association between 2,169 miRSNPs and prostate cancer risk in a large-scale analysis of 22,301 cases and 22,320 controls of European ancestry from 23 participating studies. Twenty-two miRSNPs were associated (P <2.3 × 10<sup>-5</sup>) with risk of prostate cancer, 10 of which were within 7 genes previously not mapped by GWAS studies. Further, using miRNA mimics and reporter gene assays, we showed that miR-3162-5p has specific affinity for the KLK3 rs1058205 miRSNP T-allele, whereas miR-370 has greater affinity for the VAMP8 rs1010 miRSNP A-allele, validating their functional role. SIGNIFICANCE: Findings from this large association study suggest that a focus on miRSNPs, including functional evaluation, can identify candidate risk loci below currently accepted statistical levels of genome-wide significance. Studies of miRNAs and their interactions with SNPs could provide further insights into the mechanisms of prostate cancer risk.</p>

Alkuperäiskieli	Englanti
Sivut	368-379
Sivumäärä	12
Julkaisu	CANCER DISCOVERY
Vuosikerta	5
Numero	4
DOI - pysyväislinkit	https://doi.org/10.1158/2159-8290.cd-14-1057
Tila	Julkaistu - 2015
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Julkaisufoorumi-taso

Jufo-taso 1

YK:n kestävän kehityksen tavoitteet

Tämä tuotos edistää seuraavia kestävän kehityksen tavoitteita:

Pääsy asiakirjaan

10.1158/2159-8290.cd-14-1057

Siteeraa tätä

Stegeman, S., Amankwah, E., Klein, K., O'Mara, T. A., Kim, D., Lin, H. Y., Permuth-Wey, J., Sellers, T. A., Srinivasan, S., Eeles, R., Easton, D., Kote-Jarai, Z., Al Olama, A. A., Benlloch, S., Muir, K., Giles, G. G., Wiklund, F., Gronberg, H., Haiman, C. A., ... Collins, A. (2015). A large-scale analysis of genetic variants within putative miRNA binding sites in prostate cancer. CANCER DISCOVERY, 5(4), 368-379. https://doi.org/10.1158/2159-8290.cd-14-1057

@article{20c9d90543cd44109b48644be275df19,

title = "A large-scale analysis of genetic variants within putative miRNA binding sites in prostate cancer",

abstract = "Prostate cancer is the second most common malignancy among men worldwide. Genome-wide association studies have identified 100 risk variants for prostate cancer, which can explain approximately 33% of the familial risk of the disease. We hypothesized that a comprehensive analysis of genetic variations found within the 3′ untranslated region of genes predicted to affect miRNA binding (miRSNP) can identify additional prostate cancer risk variants. We investigated the association between 2,169 miRSNPs and prostate cancer risk in a large-scale analysis of 22,301 cases and 22,320 controls of European ancestry from 23 participating studies. Twenty-two miRSNPs were associated (P <2.3 × 10-5) with risk of prostate cancer, 10 of which were within 7 genes previously not mapped by GWAS studies. Further, using miRNA mimics and reporter gene assays, we showed that miR-3162-5p has specific affinity for the KLK3 rs1058205 miRSNP T-allele, whereas miR-370 has greater affinity for the VAMP8 rs1010 miRSNP A-allele, validating their functional role. SIGNIFICANCE: Findings from this large association study suggest that a focus on miRSNPs, including functional evaluation, can identify candidate risk loci below currently accepted statistical levels of genome-wide significance. Studies of miRNAs and their interactions with SNPs could provide further insights into the mechanisms of prostate cancer risk.",

author = "Shane Stegeman and Ernest Amankwah and Kerenaftali Klein and O'Mara, {Trace A} and Donghwa Kim and Lin, {Hui Yi} and Jennifer Permuth-Wey and Sellers, {Thomas A.} and Srilakshmi Srinivasan and Rosalind Eeles and Doug Easton and Zsofia Kote-Jarai and {Al Olama}, {Ali Amin} and Sara Benlloch and Kenneth Muir and Giles, {Graham G.} and Fik Wiklund and Henrik Gronberg and Haiman, {Christopher A.} and Johanna Schleutker and Nordestgaard, {B{\o}rge G.} and Travis, {Ruth C.} and David Neal and Paul Pharoah and Khaw, {Kay Tee} and Stanford, {Janet L.} and Blot, {William J.} and Stephen Thibodeau and Christiane Maier and Kibel, {Adam S.} and Cezary Cybulski and Lisa Cannon-Albright and Hermann Brenner and Radka Kaneva and Teixeira, {Manuel R.} and Spurdle, {Amanda B.} and Clements, {Judith A.} and Park, {Jong Y.} and Jyotsna Batra and Margaret Cook and Angela Morgan and Artitaya Lophatananon and Cyril Fisher and Daniel Leongamornlert and Saunders, {Edward J.} and Sawyer, {Emma J.} and Koveela Govindasami and Malgorzata Tymrakiewicz and Michelle Guy and Naomi Livni and Rosemary Wilkinson and Sara Jugurnauth-Little and Steve Hazel and Tokhir Dadaev and John Pedersen and Hopper, {John L.} and Southey, {Melissa C.} and Ami Karlsson and Carin Cavalli-Bjoerkman and Johansson, {Jan Erik} and Jan Adolfson and Markus Aly and Michael Broms and Paer Stattin and Henderson, {Brian E.} and Fredrick Schumacher and Anssi Auvinen and Kimmo Taari and Liisa Maeaettaenen and Paula Kujala and Teemu Murtola and Tammela, {Teuvo L.J.} and Tiina Wahlfors and Andreas Roder and Peter Iversen and Peter Klarskov and Nielsen, {Sune F.} and Maren Weischer and Key, {Tim J.} and Hans Wallinder and Sven Gustafsson and Donovan, {Jenny L.} and Freddie Hamdy and Anne George and Athene Lane and Gemma Marsden and Michael Davis and Paul Brown and Nora Pashayan and Sarah Holt and Signorello, {Lisa B.} and Wei Zheng and Liang Wang and Lori Tillmans and Shaun Riska and Antje Rinckleb and Kathleen Herkommer and Manuel Luedeke and Walther Vogel and Dominika Wokozorczyk and Jan Lubiski and Wojciech Kluzniak and Dieffenbach, {Aida K.} and Christa Stegmaier and Volker Arndt and Babu Zachariah and Hyun Park and Julio Pow-Sang and Maria Rincon and Selina Radlein and Aleksandrina Vlahova and Atanaska Mitkova and Chavdar Slavov and Darina Kachakova and Elenko Popov and Svetlana Christova and Tihomir Dikov and Vanio Mitev and Felicity Lose and Joana Santos and Joao Barros-Silva and Paula Paulo and Pedro Pinto and Rui Henrique and Sofia Maia and Gail Risbridger and Renea Taylor and Wayne Tilley and Lisa Butler and Lisa Horvath and Trina Yeadon and Allison Eckert and Glen Wood and Peter Heathcote and Greg Malone and Kris Kerr and Megan Turner and Angus Collins",

year = "2015",

doi = "10.1158/2159-8290.cd-14-1057",

language = "English",

volume = "5",

pages = "368--379",

journal = "CANCER DISCOVERY",

issn = "2159-8274",

publisher = "American Association for Cancer Research Inc.",

number = "4",

}

Stegeman, S, Amankwah, E, Klein, K, O'Mara, TA, Kim, D, Lin, HY, Permuth-Wey, J, Sellers, TA, Srinivasan, S, Eeles, R, Easton, D, Kote-Jarai, Z, Al Olama, AA, Benlloch, S, Muir, K, Giles, GG, Wiklund, F, Gronberg, H, Haiman, CA, Schleutker, J, Nordestgaard, BG, Travis, RC, Neal, D, Pharoah, P, Khaw, KT, Stanford, JL, Blot, WJ, Thibodeau, S, Maier, C, Kibel, AS, Cybulski, C, Cannon-Albright, L, Brenner, H, Kaneva, R, Teixeira, MR, Spurdle, AB, Clements, JA, Park, JY, Batra, J, Cook, M, Morgan, A, Lophatananon, A, Fisher, C, Leongamornlert, D, Saunders, EJ, Sawyer, EJ, Govindasami, K, Tymrakiewicz, M, Guy, M, Livni, N, Wilkinson, R, Jugurnauth-Little, S, Hazel, S, Dadaev, T, Pedersen, J, Hopper, JL, Southey, MC, Karlsson, A, Cavalli-Bjoerkman, C, Johansson, JE, Adolfson, J, Aly, M, Broms, M, Stattin, P, Henderson, BE, Schumacher, F, Auvinen, A, Taari, K, Maeaettaenen, L, Kujala, P, Murtola, T , Tammela, TLJ, Wahlfors, T, Roder, A, Iversen, P, Klarskov, P, Nielsen, SF, Weischer, M, Key, TJ, Wallinder, H, Gustafsson, S, Donovan, JL, Hamdy, F, George, A, Lane, A, Marsden, G, Davis, M, Brown, P, Pashayan, N, Holt, S, Signorello, LB, Zheng, W, Wang, L, Tillmans, L, Riska, S, Rinckleb, A, Herkommer, K, Luedeke, M, Vogel, W, Wokozorczyk, D, Lubiski, J, Kluzniak, W, Dieffenbach, AK, Stegmaier, C, Arndt, V, Zachariah, B, Park, H, Pow-Sang, J, Rincon, M, Radlein, S, Vlahova, A, Mitkova, A, Slavov, C, Kachakova, D, Popov, E, Christova, S, Dikov, T, Mitev, V, Lose, F, Santos, J, Barros-Silva, J, Paulo, P, Pinto, P, Henrique, R, Maia, S, Risbridger, G, Taylor, R, Tilley, W, Butler, L, Horvath, L, Yeadon, T, Eckert, A, Wood, G, Heathcote, P, Malone, G, Kerr, K, Turner, M & Collins, A 2015, 'A large-scale analysis of genetic variants within putative miRNA binding sites in prostate cancer', CANCER DISCOVERY, Vuosikerta 5, Nro 4, Sivut 368-379. https://doi.org/10.1158/2159-8290.cd-14-1057

TY - JOUR

T1 - A large-scale analysis of genetic variants within putative miRNA binding sites in prostate cancer

AU - Stegeman, Shane

AU - Amankwah, Ernest

AU - Klein, Kerenaftali

AU - O'Mara, Trace A

AU - Kim, Donghwa

AU - Lin, Hui Yi

AU - Permuth-Wey, Jennifer

AU - Sellers, Thomas A.

AU - Srinivasan, Srilakshmi

AU - Eeles, Rosalind

AU - Easton, Doug

AU - Kote-Jarai, Zsofia

AU - Al Olama, Ali Amin

AU - Benlloch, Sara

AU - Muir, Kenneth

AU - Giles, Graham G.

AU - Wiklund, Fik

AU - Gronberg, Henrik

AU - Haiman, Christopher A.

AU - Schleutker, Johanna

AU - Nordestgaard, Børge G.

AU - Travis, Ruth C.

AU - Neal, David

AU - Pharoah, Paul

AU - Khaw, Kay Tee

AU - Stanford, Janet L.

AU - Blot, William J.

AU - Thibodeau, Stephen

AU - Maier, Christiane

AU - Kibel, Adam S.

AU - Cybulski, Cezary

AU - Cannon-Albright, Lisa

AU - Brenner, Hermann

AU - Kaneva, Radka

AU - Teixeira, Manuel R.

AU - Spurdle, Amanda B.

AU - Clements, Judith A.

AU - Park, Jong Y.

AU - Batra, Jyotsna

AU - Cook, Margaret

AU - Morgan, Angela

AU - Lophatananon, Artitaya

AU - Fisher, Cyril

AU - Leongamornlert, Daniel

AU - Saunders, Edward J.

AU - Sawyer, Emma J.

AU - Govindasami, Koveela

AU - Tymrakiewicz, Malgorzata

AU - Guy, Michelle

AU - Livni, Naomi

AU - Wilkinson, Rosemary

AU - Jugurnauth-Little, Sara

AU - Hazel, Steve

AU - Dadaev, Tokhir

AU - Pedersen, John

AU - Hopper, John L.

AU - Southey, Melissa C.

AU - Karlsson, Ami

AU - Cavalli-Bjoerkman, Carin

AU - Johansson, Jan Erik

AU - Adolfson, Jan

AU - Aly, Markus

AU - Broms, Michael

AU - Stattin, Paer

AU - Henderson, Brian E.

AU - Schumacher, Fredrick

AU - Auvinen, Anssi

AU - Taari, Kimmo

AU - Maeaettaenen, Liisa

AU - Kujala, Paula

AU - Murtola, Teemu

AU - Tammela, Teuvo L.J.

AU - Wahlfors, Tiina

AU - Roder, Andreas

AU - Iversen, Peter

AU - Klarskov, Peter

AU - Nielsen, Sune F.

AU - Weischer, Maren

AU - Key, Tim J.

AU - Wallinder, Hans

AU - Gustafsson, Sven

AU - Donovan, Jenny L.

AU - Hamdy, Freddie

AU - George, Anne

AU - Lane, Athene

AU - Marsden, Gemma

AU - Davis, Michael

AU - Brown, Paul

AU - Pashayan, Nora

AU - Holt, Sarah

AU - Signorello, Lisa B.

AU - Zheng, Wei

AU - Wang, Liang

AU - Tillmans, Lori

AU - Riska, Shaun

AU - Rinckleb, Antje

AU - Herkommer, Kathleen

AU - Luedeke, Manuel

AU - Vogel, Walther

AU - Wokozorczyk, Dominika

AU - Lubiski, Jan

AU - Kluzniak, Wojciech

AU - Dieffenbach, Aida K.

AU - Stegmaier, Christa

AU - Arndt, Volker

AU - Zachariah, Babu

AU - Park, Hyun

AU - Pow-Sang, Julio

AU - Rincon, Maria

AU - Radlein, Selina

AU - Vlahova, Aleksandrina

AU - Mitkova, Atanaska

AU - Slavov, Chavdar

AU - Kachakova, Darina

AU - Popov, Elenko

AU - Christova, Svetlana

AU - Dikov, Tihomir

AU - Mitev, Vanio

AU - Lose, Felicity

AU - Santos, Joana

AU - Barros-Silva, Joao

AU - Paulo, Paula

AU - Pinto, Pedro

AU - Henrique, Rui

AU - Maia, Sofia

AU - Risbridger, Gail

AU - Taylor, Renea

AU - Tilley, Wayne

AU - Butler, Lisa

AU - Horvath, Lisa

AU - Yeadon, Trina

AU - Eckert, Allison

AU - Wood, Glen

AU - Heathcote, Peter

AU - Malone, Greg

AU - Kerr, Kris

AU - Turner, Megan

AU - Collins, Angus

PY - 2015

Y1 - 2015

N2 - Prostate cancer is the second most common malignancy among men worldwide. Genome-wide association studies have identified 100 risk variants for prostate cancer, which can explain approximately 33% of the familial risk of the disease. We hypothesized that a comprehensive analysis of genetic variations found within the 3′ untranslated region of genes predicted to affect miRNA binding (miRSNP) can identify additional prostate cancer risk variants. We investigated the association between 2,169 miRSNPs and prostate cancer risk in a large-scale analysis of 22,301 cases and 22,320 controls of European ancestry from 23 participating studies. Twenty-two miRSNPs were associated (P <2.3 × 10-5) with risk of prostate cancer, 10 of which were within 7 genes previously not mapped by GWAS studies. Further, using miRNA mimics and reporter gene assays, we showed that miR-3162-5p has specific affinity for the KLK3 rs1058205 miRSNP T-allele, whereas miR-370 has greater affinity for the VAMP8 rs1010 miRSNP A-allele, validating their functional role. SIGNIFICANCE: Findings from this large association study suggest that a focus on miRSNPs, including functional evaluation, can identify candidate risk loci below currently accepted statistical levels of genome-wide significance. Studies of miRNAs and their interactions with SNPs could provide further insights into the mechanisms of prostate cancer risk.

AB - Prostate cancer is the second most common malignancy among men worldwide. Genome-wide association studies have identified 100 risk variants for prostate cancer, which can explain approximately 33% of the familial risk of the disease. We hypothesized that a comprehensive analysis of genetic variations found within the 3′ untranslated region of genes predicted to affect miRNA binding (miRSNP) can identify additional prostate cancer risk variants. We investigated the association between 2,169 miRSNPs and prostate cancer risk in a large-scale analysis of 22,301 cases and 22,320 controls of European ancestry from 23 participating studies. Twenty-two miRSNPs were associated (P <2.3 × 10-5) with risk of prostate cancer, 10 of which were within 7 genes previously not mapped by GWAS studies. Further, using miRNA mimics and reporter gene assays, we showed that miR-3162-5p has specific affinity for the KLK3 rs1058205 miRSNP T-allele, whereas miR-370 has greater affinity for the VAMP8 rs1010 miRSNP A-allele, validating their functional role. SIGNIFICANCE: Findings from this large association study suggest that a focus on miRSNPs, including functional evaluation, can identify candidate risk loci below currently accepted statistical levels of genome-wide significance. Studies of miRNAs and their interactions with SNPs could provide further insights into the mechanisms of prostate cancer risk.

U2 - 10.1158/2159-8290.cd-14-1057

DO - 10.1158/2159-8290.cd-14-1057

M3 - Article

AN - SCOPUS:85000352081

SN - 2159-8274

VL - 5

SP - 368

EP - 379

JO - CANCER DISCOVERY

JF - CANCER DISCOVERY

IS - 4

ER -

A large-scale analysis of genetic variants within putative miRNA binding sites in prostate cancer

Abstrakti

Julkaisufoorumi-taso

YK:n kestävän kehityksen tavoitteet

Pääsy asiakirjaan

Sormenjälki

Siteeraa tätä