Aggregation versus Biological Activity in Gold(I) Complexes. An Unexplored Concept

Andrea Pinto; Catarina Roma-Rodrigues; Jas S. Ward; Rakesh Puttreddy; Kari Rissanen; Pedro V. Baptista; Alexandra R. Fernandes; João Carlos Lima; Laura Rodríguez

doi:10.1021/acs.inorgchem.1c02359

Aggregation versus Biological Activity in Gold(I) Complexes. An Unexplored Concept

Andrea Pinto
, Catarina Roma-Rodrigues
, Jas S. Ward
, Rakesh Puttreddy
, Kari Rissanen
, Pedro V. Baptista
, Alexandra R. Fernandes
, João Carlos Lima
, Laura Rodríguez^*

^*Tämän työn vastaava kirjoittaja

Tutkimustuotos: Artikkeli › Tieteellinen › vertaisarvioitu

12 Sitaatiot (Scopus)

Abstrakti

The aggregation process of a series of mono- and dinuclear gold(I) complexes containing a 4-ethynylaniline ligand and a phosphane at the second coordination position (PR3-Au-CCC6H4-NH2, complexes 1-5, and (diphos)(Au-CCC6H4-NH2)2, complexes 6-8), whose biological activity was previously studied by us, has been carefully analyzed through absorption, emission, and NMR spectroscopy, together with dynamic light scattering and small-angle X-ray scattering. These experiments allow us to retrieve information about how the compounds enter the cells. It was observed that all compounds present aggregation in fresh solutions, before biological treatment, and thus they must be entering the cells as aggregates. Inductively coupled plasma atomic emission spectrometry measurements showed that mononuclear complexes are mainly found in the cytosolic fraction; the dinuclear complexes are mainly found in a subsequent fraction composed of nuclei and cytoskeleton. Additionally, dinuclear complex 8 affects the actin aggregation to a larger extent, suggesting a cooperative effect of dinuclear compounds.

Alkuperäiskieli	Englanti
Sivut	18753–18763
Julkaisu	Inorganic Chemistry
Vuosikerta	60
Numero	24
Varhainen verkossa julkaisun päivämäärä	marrask. 2021
DOI - pysyväislinkit	https://doi.org/10.1021/acs.inorgchem.1c02359
Tila	Julkaistu - jouluk. 2021
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Rahoitus

The authors are grateful to the Spanish Ministerio de Ciencia, Innovación y Universidades (Project PID2019-104121GB-I00). This work was supported by the Associate Laboratory for Green Chemistry, LAQV, which is financed by national funds from FCT/MCTES (Grants UIDB/50006/2020 and UIDP/50006/2020). This work was also financed by national funds from Fundação para a Ciência e a Tecnologia, I.P., in the scope of Projects UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences, UCIBIO, and Project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy, i4HB. SAXS experiments were performed at the NCD-BL11 beamline of the ALBA Synchrotron Light Facility in collaboration with the ALBA staff. The authors also acknowledge COST Actions CA1740─Nano4clinics and CA18202─NECTAR. The Finnish Cultural Foundation (Grant 00201148 to J.S.W.) is also acknowledged for their support.

Julkaisufoorumi-taso

Jufo-taso 2

!!ASJC Scopus subject areas

Physical and Theoretical Chemistry
Inorganic Chemistry

Pääsy asiakirjaan

10.1021/acs.inorgchem.1c02359

CCDC 2070640: Experimental Crystal Structure Determination
Pinto, A. (Contributor), Roma-Rodrigues, C. (Contributor), Ward, J. S. (Contributor), Puttreddy, R. (Contributor), Rissanen, K. (Creator), Baptista, P. V. (Contributor), Fernandes, A. R. (Contributor) & Rodríguez, L. (Contributor), Cambridge Crystallographic Data Centre, 16 maalisk. 2021
DOI - pysyväislinkki: 10.5517/ccdc.csd.cc27hnv8
Tietoaineisto: Dataset
CCDC 2070638: Experimental Crystal Structure Determination
Pinto, A. (Contributor), Roma-Rodrigues, C. (Contributor), Ward, J. S. (Contributor), Puttreddy, R. (Contributor), Rissanen, K. (Creator), Baptista, P. V. (Contributor), Fernandes, A. R. (Contributor) & Rodríguez, L. (Contributor), Cambridge Crystallographic Data Centre, 16 maalisk. 2021
DOI - pysyväislinkki: 10.5517/ccdc.csd.cc27hns6
Tietoaineisto: Dataset
CCDC 2070639: Experimental Crystal Structure Determination
Pinto, A. (Contributor), Roma-Rodrigues, C. (Contributor), Ward, J. S. (Contributor), Puttreddy, R. (Contributor), Rissanen, K. (Creator), Baptista, P. V. (Contributor), Fernandes, A. R. (Contributor) & Rodríguez, L. (Contributor), Cambridge Crystallographic Data Centre, 16 maalisk. 2021
DOI - pysyväislinkki: 10.5517/ccdc.csd.cc27hnt7
Tietoaineisto: Dataset

Siteeraa tätä

@article{232596ba841b46288a1ee39968f4f66c,

title = "Aggregation versus Biological Activity in Gold(I) Complexes. An Unexplored Concept",

abstract = "The aggregation process of a series of mono- and dinuclear gold(I) complexes containing a 4-ethynylaniline ligand and a phosphane at the second coordination position (PR3-Au-CCC6H4-NH2, complexes 1-5, and (diphos)(Au-CCC6H4-NH2)2, complexes 6-8), whose biological activity was previously studied by us, has been carefully analyzed through absorption, emission, and NMR spectroscopy, together with dynamic light scattering and small-angle X-ray scattering. These experiments allow us to retrieve information about how the compounds enter the cells. It was observed that all compounds present aggregation in fresh solutions, before biological treatment, and thus they must be entering the cells as aggregates. Inductively coupled plasma atomic emission spectrometry measurements showed that mononuclear complexes are mainly found in the cytosolic fraction; the dinuclear complexes are mainly found in a subsequent fraction composed of nuclei and cytoskeleton. Additionally, dinuclear complex 8 affects the actin aggregation to a larger extent, suggesting a cooperative effect of dinuclear compounds. ",

author = "Andrea Pinto and Catarina Roma-Rodrigues and Ward, \{Jas S.\} and Rakesh Puttreddy and Kari Rissanen and Baptista, \{Pedro V.\} and Fernandes, \{Alexandra R.\} and Lima, \{Jo{\~a}o Carlos\} and Laura Rodr{\'i}guez",

note = "Funding Information: The authors are grateful to the Spanish Ministerio de Ciencia, Innovaci{\'o}n y Universidades (Project PID2019-104121GB-I00). This work was supported by the Associate Laboratory for Green Chemistry, LAQV, which is financed by national funds from FCT/MCTES (Grants UIDB/50006/2020 and UIDP/50006/2020). This work was also financed by national funds from Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia, I.P., in the scope of Projects UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences, UCIBIO, and Project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy, i4HB. SAXS experiments were performed at the NCD-BL11 beamline of the ALBA Synchrotron Light Facility in collaboration with the ALBA staff. The authors also acknowledge COST Actions CA1740─Nano4clinics and CA18202─NECTAR. The Finnish Cultural Foundation (Grant 00201148 to J.S.W.) is also acknowledged for their support. Publisher Copyright: {\textcopyright} 2021 American Chemical Society.",

year = "2021",

month = dec,

doi = "10.1021/acs.inorgchem.1c02359",

language = "English",

volume = "60",

pages = "18753–18763",

journal = "Inorganic Chemistry",

issn = "0020-1669",

publisher = "American Chemical Society",

number = "24",

}

TY - JOUR

T1 - Aggregation versus Biological Activity in Gold(I) Complexes. An Unexplored Concept

AU - Pinto, Andrea

AU - Roma-Rodrigues, Catarina

AU - Ward, Jas S.

AU - Puttreddy, Rakesh

AU - Rissanen, Kari

AU - Baptista, Pedro V.

AU - Fernandes, Alexandra R.

AU - Lima, João Carlos

AU - Rodríguez, Laura

N1 - Funding Information: The authors are grateful to the Spanish Ministerio de Ciencia, Innovación y Universidades (Project PID2019-104121GB-I00). This work was supported by the Associate Laboratory for Green Chemistry, LAQV, which is financed by national funds from FCT/MCTES (Grants UIDB/50006/2020 and UIDP/50006/2020). This work was also financed by national funds from Fundação para a Ciência e a Tecnologia, I.P., in the scope of Projects UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences, UCIBIO, and Project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy, i4HB. SAXS experiments were performed at the NCD-BL11 beamline of the ALBA Synchrotron Light Facility in collaboration with the ALBA staff. The authors also acknowledge COST Actions CA1740─Nano4clinics and CA18202─NECTAR. The Finnish Cultural Foundation (Grant 00201148 to J.S.W.) is also acknowledged for their support. Publisher Copyright: © 2021 American Chemical Society.

PY - 2021/12

Y1 - 2021/12

N2 - The aggregation process of a series of mono- and dinuclear gold(I) complexes containing a 4-ethynylaniline ligand and a phosphane at the second coordination position (PR3-Au-CCC6H4-NH2, complexes 1-5, and (diphos)(Au-CCC6H4-NH2)2, complexes 6-8), whose biological activity was previously studied by us, has been carefully analyzed through absorption, emission, and NMR spectroscopy, together with dynamic light scattering and small-angle X-ray scattering. These experiments allow us to retrieve information about how the compounds enter the cells. It was observed that all compounds present aggregation in fresh solutions, before biological treatment, and thus they must be entering the cells as aggregates. Inductively coupled plasma atomic emission spectrometry measurements showed that mononuclear complexes are mainly found in the cytosolic fraction; the dinuclear complexes are mainly found in a subsequent fraction composed of nuclei and cytoskeleton. Additionally, dinuclear complex 8 affects the actin aggregation to a larger extent, suggesting a cooperative effect of dinuclear compounds.

AB - The aggregation process of a series of mono- and dinuclear gold(I) complexes containing a 4-ethynylaniline ligand and a phosphane at the second coordination position (PR3-Au-CCC6H4-NH2, complexes 1-5, and (diphos)(Au-CCC6H4-NH2)2, complexes 6-8), whose biological activity was previously studied by us, has been carefully analyzed through absorption, emission, and NMR spectroscopy, together with dynamic light scattering and small-angle X-ray scattering. These experiments allow us to retrieve information about how the compounds enter the cells. It was observed that all compounds present aggregation in fresh solutions, before biological treatment, and thus they must be entering the cells as aggregates. Inductively coupled plasma atomic emission spectrometry measurements showed that mononuclear complexes are mainly found in the cytosolic fraction; the dinuclear complexes are mainly found in a subsequent fraction composed of nuclei and cytoskeleton. Additionally, dinuclear complex 8 affects the actin aggregation to a larger extent, suggesting a cooperative effect of dinuclear compounds.

U2 - 10.1021/acs.inorgchem.1c02359

DO - 10.1021/acs.inorgchem.1c02359

M3 - Article

AN - SCOPUS:85118956456

SN - 0020-1669

VL - 60

SP - 18753

EP - 18763

JO - Inorganic Chemistry

JF - Inorganic Chemistry

IS - 24

ER -

Aggregation versus Biological Activity in Gold(I) Complexes. An Unexplored Concept

Abstrakti

Rahoitus

Julkaisufoorumi-taso

!!ASJC Scopus subject areas

Pääsy asiakirjaan

Sormenjälki

Tietoaineistot

CCDC 2070640: Experimental Crystal Structure Determination

CCDC 2070638: Experimental Crystal Structure Determination

CCDC 2070639: Experimental Crystal Structure Determination

Siteeraa tätä