Ammonia tolerant inocula provide a good base for anaerobic digestion of microalgae in third generation biogas process

Ahmed Mahdy; Ioannis A. Fotidis; Enrico Mancini; Mercedes Ballesteros; Cristina González-Fernández; Irini Angelidaki

doi:10.1016/j.biortech.2016.11.086

Ammonia tolerant inocula provide a good base for anaerobic digestion of microalgae in third generation biogas process

Ahmed Mahdy
, Ioannis A. Fotidis^*
, Enrico Mancini
, Mercedes Ballesteros
, Cristina González-Fernández
, Irini Angelidaki

^*Tämän työn vastaava kirjoittaja

Tutkimustuotos: Artikkeli › Tieteellinen › vertaisarvioitu

76 Sitaatiot (Scopus)

Abstrakti

This study investigated the ability of an ammonia-acclimatized inoculum to digest efficiently protein-rich microalgae for continuous 3rd generation biogas production. Moreover, we investigated whether increased C/N ratio could alleviate ammonia toxicity. The biochemical methane potential (BMP) of five different algae (Chlorella vulgaris)/manure (cattle) mixtures showed that the mixture of 80/20 (on VS basis) resulted in the highest BMP value (431 mL CH₄ g VS⁻¹), while the BMP of microalgae alone (100/0) was 415 mL CH₄ g VS⁻¹. Subsequently, anaerobic digestion of those two substrates was tested in continuous stirred tank reactors (CSTR). Despite of the high ammonium levels (3.7–4.2 g NH₄⁺-N L⁻¹), CSTR reactors using ammonia tolerant inoculum resulted in relatively high methane yields (i.e. 77.5% and 84% of the maximum expected, respectively). These results demonstrated that ammonia tolerant inocula could be a promising approach to successfully digest protein-rich microalgae and achieve a 3rd generation biogas production.

Alkuperäiskieli	Englanti
Sivut	272-278
Sivumäärä	7
Julkaisu	Bioresource Technology
Vuosikerta	225
DOI - pysyväislinkit	https://doi.org/10.1016/j.biortech.2016.11.086
Tila	Julkaistu - helmik. 2017
Julkaistu ulkoisesti	Kyllä
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Rahoitus

This work was supported by Energinet.dk under the project framework ForskEL “MicrobStopNH 3 -Innovative bioaugmentation strategies to tackle ammonia inhibition in anaerobic digestion process” (program no. 2015-12327) and the Spanish Ministry of Economy and Competitiveness for financial support to projects of (WW-ALGAS, ENE2013-45416-R and RYC-2014-16823). We would also like to acknowledge the Community of Madrid for the support offered in the framework of the project INSPIRA-1 (S2013/ABI-2783). The authors would like to extend their gratitude to Novozymes-Denmark.

YK:n kestävän kehityksen tavoitteet

Tämä tuotos edistää seuraavia kestävän kehityksen tavoitteita:

SDG 7 – Edullinen ja puhdas energia

!!ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

Pääsy asiakirjaan

10.1016/j.biortech.2016.11.086

Siteeraa tätä

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title = "Ammonia tolerant inocula provide a good base for anaerobic digestion of microalgae in third generation biogas process",

abstract = "This study investigated the ability of an ammonia-acclimatized inoculum to digest efficiently protein-rich microalgae for continuous 3rd generation biogas production. Moreover, we investigated whether increased C/N ratio could alleviate ammonia toxicity. The biochemical methane potential (BMP) of five different algae (Chlorella vulgaris)/manure (cattle) mixtures showed that the mixture of 80/20 (on VS basis) resulted in the highest BMP value (431 mL CH4 g VS−1), while the BMP of microalgae alone (100/0) was 415 mL CH4 g VS−1. Subsequently, anaerobic digestion of those two substrates was tested in continuous stirred tank reactors (CSTR). Despite of the high ammonium levels (3.7–4.2 g NH4+-N L−1), CSTR reactors using ammonia tolerant inoculum resulted in relatively high methane yields (i.e. 77.5\% and 84\% of the maximum expected, respectively). These results demonstrated that ammonia tolerant inocula could be a promising approach to successfully digest protein-rich microalgae and achieve a 3rd generation biogas production.",

keywords = "Ammonia inhibition, Anaerobic digestion, BMP, Co-digestion, Microalgae",

author = "Ahmed Mahdy and Fotidis, \{Ioannis A.\} and Enrico Mancini and Mercedes Ballesteros and Cristina Gonz{\'a}lez-Fern{\'a}ndez and Irini Angelidaki",

note = "Funding Information: This work was supported by Energinet.dk under the project framework ForskEL “MicrobStopNH 3 -Innovative bioaugmentation strategies to tackle ammonia inhibition in anaerobic digestion process” (program no. 2015-12327) and the Spanish Ministry of Economy and Competitiveness for financial support to projects of (WW-ALGAS, ENE2013-45416-R and RYC-2014-16823). We would also like to acknowledge the Community of Madrid for the support offered in the framework of the project INSPIRA-1 (S2013/ABI-2783). The authors would like to extend their gratitude to Novozymes-Denmark. Publisher Copyright: {\textcopyright} 2016 Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2017",

month = feb,

doi = "10.1016/j.biortech.2016.11.086",

language = "English",

volume = "225",

pages = "272--278",

journal = "Bioresource Technology",

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TY - JOUR

T1 - Ammonia tolerant inocula provide a good base for anaerobic digestion of microalgae in third generation biogas process

AU - Mahdy, Ahmed

AU - Fotidis, Ioannis A.

AU - Mancini, Enrico

AU - Ballesteros, Mercedes

AU - González-Fernández, Cristina

AU - Angelidaki, Irini

N1 - Funding Information: This work was supported by Energinet.dk under the project framework ForskEL “MicrobStopNH 3 -Innovative bioaugmentation strategies to tackle ammonia inhibition in anaerobic digestion process” (program no. 2015-12327) and the Spanish Ministry of Economy and Competitiveness for financial support to projects of (WW-ALGAS, ENE2013-45416-R and RYC-2014-16823). We would also like to acknowledge the Community of Madrid for the support offered in the framework of the project INSPIRA-1 (S2013/ABI-2783). The authors would like to extend their gratitude to Novozymes-Denmark. Publisher Copyright: © 2016 Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2017/2

Y1 - 2017/2

N2 - This study investigated the ability of an ammonia-acclimatized inoculum to digest efficiently protein-rich microalgae for continuous 3rd generation biogas production. Moreover, we investigated whether increased C/N ratio could alleviate ammonia toxicity. The biochemical methane potential (BMP) of five different algae (Chlorella vulgaris)/manure (cattle) mixtures showed that the mixture of 80/20 (on VS basis) resulted in the highest BMP value (431 mL CH4 g VS−1), while the BMP of microalgae alone (100/0) was 415 mL CH4 g VS−1. Subsequently, anaerobic digestion of those two substrates was tested in continuous stirred tank reactors (CSTR). Despite of the high ammonium levels (3.7–4.2 g NH4+-N L−1), CSTR reactors using ammonia tolerant inoculum resulted in relatively high methane yields (i.e. 77.5% and 84% of the maximum expected, respectively). These results demonstrated that ammonia tolerant inocula could be a promising approach to successfully digest protein-rich microalgae and achieve a 3rd generation biogas production.

AB - This study investigated the ability of an ammonia-acclimatized inoculum to digest efficiently protein-rich microalgae for continuous 3rd generation biogas production. Moreover, we investigated whether increased C/N ratio could alleviate ammonia toxicity. The biochemical methane potential (BMP) of five different algae (Chlorella vulgaris)/manure (cattle) mixtures showed that the mixture of 80/20 (on VS basis) resulted in the highest BMP value (431 mL CH4 g VS−1), while the BMP of microalgae alone (100/0) was 415 mL CH4 g VS−1. Subsequently, anaerobic digestion of those two substrates was tested in continuous stirred tank reactors (CSTR). Despite of the high ammonium levels (3.7–4.2 g NH4+-N L−1), CSTR reactors using ammonia tolerant inoculum resulted in relatively high methane yields (i.e. 77.5% and 84% of the maximum expected, respectively). These results demonstrated that ammonia tolerant inocula could be a promising approach to successfully digest protein-rich microalgae and achieve a 3rd generation biogas production.

KW - Ammonia inhibition

KW - Anaerobic digestion

KW - BMP

KW - Co-digestion

KW - Microalgae

U2 - 10.1016/j.biortech.2016.11.086

DO - 10.1016/j.biortech.2016.11.086

M3 - Article

C2 - 27898317

AN - SCOPUS:84998911398

SN - 0960-8524

VL - 225

SP - 272

EP - 278

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Ammonia tolerant inocula provide a good base for anaerobic digestion of microalgae in third generation biogas process

Abstrakti

Rahoitus

YK:n kestävän kehityksen tavoitteet

!!ASJC Scopus subject areas

Pääsy asiakirjaan

Sormenjälki

Siteeraa tätä