Analysis of Ca2+ and cAMP signaling regulated by GPR17 at single cell level.

G Protein‐Coupled Receptors signal transduction pathways plays crucial role in the many neuronal diseases including Alzheimer’s disease, Parkinson’s disease and Multiple Sclerosis. Recently, GPR17 found to be a unique target for human
neurodegenerative disease. This receptor is typically present in neurons and acts as a classical ligand‐activated GPCR, which responds to uracil nucleotides and cysteinyl‐leukotrienes. GPR17 acts as a mediator in neurotic progression as well
as involved in cell death mechanism in diseased state. Thus, GPR17 is considered as a predetermined therapeutic target. Existence of divergent functions of GPR17 been reported. It was previously reported that activation of GPR17 leads to the adenylyl cyclase inhibition which also results in the increased intracellular calcium concentration. However, detailed molecular mechanism of GPR17 not well‐studied. The central focus of this study is to analyse Ca2+ and cyclic AMP level regulated by GPR17 at single cell level using biosensors and computational biology approaches. For this we optimized the transfection method and single cell imaging protocol which allows us to detect minimal changes in single cell. In the present report we will discuss our preliminary finding on how GPR17 signalling affects Ca2+ and cAMP at single cell level.