@article{a18d9d6278c44c2584e56cece81d28ae,
title = "Arginine Methyltransferase PRMT7 Deregulates Expression of RUNX1 Target Genes in T-Cell Acute Lymphoblastic Leukemia",
abstract = "T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy with no well-established prognostic biomarkers. We examined the expression of protein arginine methyltransferases across hematological malignancies and discovered high levels of PRMT7 mRNA in T-ALL, particularly in the mature subtypes of T-ALL. The genetic deletion of PRMT7 by CRISPR-Cas9 reduced the colony formation of T-ALL cells and changed arginine monomethylation patterns in protein complexes associated with the RNA and DNA processing and the T-ALL pathogenesis. Among them was RUNX1, whose target gene expression was consequently deregulated. These results suggest that PRMT7 plays an active role in the pathogenesis of T-ALL.",
keywords = "arginine methylation, leukemia, PRMT7, RUNX1, T-ALL",
author = "Laura Oksa and Artturi M{\"a}kinen and Atte Nikkil{\"a} and Noora Hyv{\"a}rinen and Saara Laukkanen and Anne Rokka and Pekka Haapaniemi and Masafumi Seki and Junko Takita and Otto Kauko and Merja Hein{\"a}niemi and Olli Lohi",
note = "Funding Information: Funding: This work was supported by grants from the Academy of Finland (O.L. and M.H. 321553, O.L., 310106 and 341540), Cancer Foundation Finland (O.L., M.H), Jane and Aatos Erkko Foundation (O.L., M.H.), Sigrid Juselius Foundation (O.L., M.H.), Finnish Hematology Association (L.O.), and the Competitive State Research Financing of the Expert Responsibility Area of Tampere University Hospital (O.L. 9V033 and 9X027). Funding Information: Acknowledgments: The authors acknowledge the Tampere facility of Flow Cytometry, Tampere facility of iPS Cells, Tampere Imaging Facility and the Biocenter Finland (BF) for their services. Jan Cools and Charles de Bock are acknowledged for providing the Jurkat Cas9 cells and px321-GFP - plasmids for CRISPR studies. Mass spectrometry analysis was performed at the Turku Proteomics Facility, University of Turku and {\AA}bo Akademi University. The facility is supported by Biocenter Finland. Part of the work was carried out with the support of UEF Bioinformatics Center, University of Eastern Finland, Finland. We wish to thank Jorma Kulmala, Eini Eskola, and Gitte Gaethofs for assistance in laboratory work. Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2022",
doi = "10.3390/cancers14092169",
language = "English",
volume = "14",
number = "9",
}
