Chromatin organization regulates viral egress dynamics

V Aho, Markko Myllys, Visa Ruokolainen, Satu Hakanen, Elina Mäntylä, Jori Virtanen, Veijo Hukkanen, T Kühn, Jussi Timonen, Keijo Mattila, Carolyn A. Larabell, Maija Vihinen-Ranta

    Tutkimustuotos: ArtikkeliTieteellinenvertaisarvioitu

    23 Sitaatiot (Scopus)
    79 Lataukset (Pure)

    Abstrakti

    Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion to analyse the molecular organization of chromatin in herpes simplex virus 1 infection and its effect on the transport of progeny viral capsids to the nuclear envelope. Our data showed that the formation of the viral replication compartment at late infection resulted in the enrichment of heterochromatin in the nuclear periphery accompanied by the compaction of chromatin. Random walk modelling of herpes simplex virus 1-sized particles in a three-dimensional soft x-ray tomography reconstruction of an infected cell nucleus demonstrated that the peripheral, compacted chromatin restricts viral capsid diffusion, but due to interchromatin channels capsids are able to reach the nuclear envelope, the site of their nuclear egress.

    AlkuperäiskieliEnglanti
    Artikkeli3692
    JulkaisuScientific Reports
    Vuosikerta7
    Numero1
    DOI - pysyväislinkit
    TilaJulkaistu - 2017
    OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

    Tutkimusalat

    • Herpesvirus
    • chromatin dynamics
    • nuclear egress
    • nuclear export

    Julkaisufoorumi-taso

    • Jufo-taso 2

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