Concomitant silanization and controlled fibronectin adsorption on S53P4 bioactive glass enhances human adipose stem cells spreading and differentiation

Virginia Alessandra Gobbo; Amel Houaoui; Kimiya Tajik; Vesa P. Hytönen; Susanna Miettinen; Jonathan Massera

doi:10.1016/j.apsadv.2024.100635

Concomitant silanization and controlled fibronectin adsorption on S53P4 bioactive glass enhances human adipose stem cells spreading and differentiation

Virginia Alessandra Gobbo^*
, Amel Houaoui
, Kimiya Tajik
, Vesa P. Hytönen
, Susanna Miettinen
, Jonathan Massera

^*Tämän työn vastaava kirjoittaja

Tutkimustuotos: Artikkeli › Tieteellinen › vertaisarvioitu

4 Sitaatiot (Scopus)

29 Lataukset (Pure)

Abstrakti

Orthopedic disorders are increasing in our society due to population aging. Numerous biomaterials have been developed to support bone regeneration, however showing a strong discrepancy between in vitro and in vivo results. This has been attributed to a lack of knowledge about protein adsorption, an early step occurring after biomaterial implantation. Bioactive glass S53P4 is clinically accepted for orthopedic applications pertaining to its osteoconductive and osteogenic behavior. However, its interactions with proteins are still unclear. To better understand the impact of surface chemistry on the glass-protein interactions, bare and silanized S53P4 were placed in contact with fibronectin (fn), in static and dynamic conditions. The surfaces were characterized by zeta potential, confocal microscopy and FTIR-ATR spectroscopy.The impact of fn on the cell response was assessed by live-dead, proliferation and morphology tests, using human adipose stem cells (hASCs). Both S53P4 and silanized-S53P4 showed good cell viability. Fn was found to affect cell alignment on both bare and silanized substrates. The impact of the surface treatments on osteogenesis was evaluated studying the expression of relevant osteogenic markers (hDLX5, hRUNX2A, hOSTERIX), which was particularly promoted by the concomitant action of silanization and fn coating.

Alkuperäiskieli	Englanti
Artikkeli	100635
Sivumäärä	21
Julkaisu	Applied Surface Science Advances
Vuosikerta	23
DOI - pysyväislinkit	https://doi.org/10.1016/j.apsadv.2024.100635
Tila	Julkaistu - syysk. 2024
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Rahoitus

This project was funded by the European Union's Horizon 2020 Research and Innovation Program under the Marie Sk\u0142odowska-Curie grant agreement [No 860462 (PREMUROSA)] and by Tampere University Doctoral School Programme. The authors acknowledge Niklas K\u00E4hk\u00F6nen for his assistance in protein extraction, Anna-Maija Honkala for her support in performing the gene expression tests and PhD candidate Birhanu Belay for the support in developing the methodology to study cell orientation. PhD Rolle Rahikainen is acknowledged for his support in image processing and PhD Vijay Singh Parihar for his support in the electrophoretic analysis. The authors acknowledge Prof. Emmanuel Pauthe for a fruitful discussion concerning protein conformation. Tampere Imaging Facility (TIF) and Tampere Protein Services core are acknowledged for their infrastructure support, both supported by Biocenter Finland. VPH acknowledges Academy of Finland, Sigrid Juselius Foundation and Cancer Foundation Finland for financial support.

Rahoittajat	Rahoittajan numero
Tampere Imaging Facility
Strategic Research Council at the Research Council of Finland
Syöpäsäätiö
Biocenter Finland
Sigrid Juséliuksen Säätiö
Horizon 2020
H2020 Marie Skłodowska-Curie Actions	860462

Julkaisufoorumi-taso

Jufo-taso 1

!!ASJC Scopus subject areas

Surfaces and Interfaces
Surfaces, Coatings and Films

Pääsy asiakirjaan

10.1016/j.apsadv.2024.100635Lisenssi: CC BY

1-s2.0-S2666523924000631-mainFinal published version, 12 MBLisenssi: CC BY

https://urn.fi/URN:NBN:fi:tuni-202409178752Lisenssi: CC BY

Siteeraa tätä

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title = "Concomitant silanization and controlled fibronectin adsorption on S53P4 bioactive glass enhances human adipose stem cells spreading and differentiation",

abstract = "Orthopedic disorders are increasing in our society due to population aging. Numerous biomaterials have been developed to support bone regeneration, however showing a strong discrepancy between in vitro and in vivo results. This has been attributed to a lack of knowledge about protein adsorption, an early step occurring after biomaterial implantation. Bioactive glass S53P4 is clinically accepted for orthopedic applications pertaining to its osteoconductive and osteogenic behavior. However, its interactions with proteins are still unclear. To better understand the impact of surface chemistry on the glass-protein interactions, bare and silanized S53P4 were placed in contact with fibronectin (fn), in static and dynamic conditions. The surfaces were characterized by zeta potential, confocal microscopy and FTIR-ATR spectroscopy.The impact of fn on the cell response was assessed by live-dead, proliferation and morphology tests, using human adipose stem cells (hASCs). Both S53P4 and silanized-S53P4 showed good cell viability. Fn was found to affect cell alignment on both bare and silanized substrates. The impact of the surface treatments on osteogenesis was evaluated studying the expression of relevant osteogenic markers (hDLX5, hRUNX2A, hOSTERIX), which was particularly promoted by the concomitant action of silanization and fn coating.",

keywords = "Bioactive glass, Biological response, Protein adsorption, Protein conformation, Surface modification",

author = "Gobbo, \{Virginia Alessandra\} and Amel Houaoui and Kimiya Tajik and Hyt{\"o}nen, \{Vesa P.\} and Susanna Miettinen and Jonathan Massera",

note = "Publisher Copyright: {\textcopyright} 2024",

year = "2024",

month = sep,

doi = "10.1016/j.apsadv.2024.100635",

language = "English",

volume = "23",

journal = "Applied Surface Science Advances",

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TY - JOUR

T1 - Concomitant silanization and controlled fibronectin adsorption on S53P4 bioactive glass enhances human adipose stem cells spreading and differentiation

AU - Gobbo, Virginia Alessandra

AU - Houaoui, Amel

AU - Tajik, Kimiya

AU - Hytönen, Vesa P.

AU - Miettinen, Susanna

AU - Massera, Jonathan

PY - 2024/9

Y1 - 2024/9

N2 - Orthopedic disorders are increasing in our society due to population aging. Numerous biomaterials have been developed to support bone regeneration, however showing a strong discrepancy between in vitro and in vivo results. This has been attributed to a lack of knowledge about protein adsorption, an early step occurring after biomaterial implantation. Bioactive glass S53P4 is clinically accepted for orthopedic applications pertaining to its osteoconductive and osteogenic behavior. However, its interactions with proteins are still unclear. To better understand the impact of surface chemistry on the glass-protein interactions, bare and silanized S53P4 were placed in contact with fibronectin (fn), in static and dynamic conditions. The surfaces were characterized by zeta potential, confocal microscopy and FTIR-ATR spectroscopy.The impact of fn on the cell response was assessed by live-dead, proliferation and morphology tests, using human adipose stem cells (hASCs). Both S53P4 and silanized-S53P4 showed good cell viability. Fn was found to affect cell alignment on both bare and silanized substrates. The impact of the surface treatments on osteogenesis was evaluated studying the expression of relevant osteogenic markers (hDLX5, hRUNX2A, hOSTERIX), which was particularly promoted by the concomitant action of silanization and fn coating.

AB - Orthopedic disorders are increasing in our society due to population aging. Numerous biomaterials have been developed to support bone regeneration, however showing a strong discrepancy between in vitro and in vivo results. This has been attributed to a lack of knowledge about protein adsorption, an early step occurring after biomaterial implantation. Bioactive glass S53P4 is clinically accepted for orthopedic applications pertaining to its osteoconductive and osteogenic behavior. However, its interactions with proteins are still unclear. To better understand the impact of surface chemistry on the glass-protein interactions, bare and silanized S53P4 were placed in contact with fibronectin (fn), in static and dynamic conditions. The surfaces were characterized by zeta potential, confocal microscopy and FTIR-ATR spectroscopy.The impact of fn on the cell response was assessed by live-dead, proliferation and morphology tests, using human adipose stem cells (hASCs). Both S53P4 and silanized-S53P4 showed good cell viability. Fn was found to affect cell alignment on both bare and silanized substrates. The impact of the surface treatments on osteogenesis was evaluated studying the expression of relevant osteogenic markers (hDLX5, hRUNX2A, hOSTERIX), which was particularly promoted by the concomitant action of silanization and fn coating.

KW - Bioactive glass

KW - Biological response

KW - Protein adsorption

KW - Protein conformation

KW - Surface modification

U2 - 10.1016/j.apsadv.2024.100635

DO - 10.1016/j.apsadv.2024.100635

M3 - Article

AN - SCOPUS:85203005608

SN - 2666-5239

VL - 23

JO - Applied Surface Science Advances

JF - Applied Surface Science Advances

M1 - 100635

ER -

Concomitant silanization and controlled fibronectin adsorption on S53P4 bioactive glass enhances human adipose stem cells spreading and differentiation

Abstrakti

Rahoitus

Julkaisufoorumi-taso

!!ASJC Scopus subject areas

Pääsy asiakirjaan

Sormenjälki

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